AB184961

Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal EXOSC3 antibody. N-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

RRP40, CGI-102, EXOSC3, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human colon tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling EXOSC3 with ab184961 at 1/100 dilution (red). Counterstained with Dapi (blue).

Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixedHeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.

All lanes:

Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

All lanes:

Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution

Lane 1:

293T cell lysates at 20 µg

Lane 2:

HeLa cell lysates at 20 µg

Lane 3:

Jurkat cell lysates at 20 µg

Lane 4:

Raji cell lysates at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 30 kDa

false

Carrier free

Anti-EXOSC3 antibody [EPR14840(B)] - BSA and Azide free
578 PE

PE Anti-EXOSC3 antibody [EPR14840(B)] (N-terminal)
660 APC

APC Anti-EXOSC3 antibody [EPR14840(B)] (N-terminal)
HRP

HRP Anti-EXOSC3 antibody [EPR14840(B)] (N-terminal)
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-EXOSC3 antibody [EPR14840(B)] N-terminal
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14840(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IP, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.

Biological function summary

EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.

Pathways

The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.

Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.

See full target information EXOSC3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Acta pharmacologica Sinica 46:777-793 PubMed39496863

2024

Intestinal human carboxylesterase 2 (CES2) expression rescues drug metabolism and most metabolic syndrome phenotypes in global Ces2 cluster knockout mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yao-Geng Wang,Chang-Pei Gan,Joke Beukers-Korver,Hilde Rosing,Wen-Long Li,Els Wagenaar,Maria C Lebre,Ji-Ying Song,Colin Pritchard,Rahmen Bin Ali,Ivo Huijbers,Jos H Beijnen,Alfred H Schinkel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise