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Rabbit Recombinant Monoclonal EXOSC3 antibody. N-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961), expandable thumbnail
  • Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961), expandable thumbnail
  • Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (AB184961), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Rat
Predicted
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/50 - 1/70

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/10000 - 1/50000

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.

Alternative names

Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10, CGI-102, EXOSC3, RRP40

Recommended products

Rabbit Recombinant Monoclonal EXOSC3 antibody. N-terminal. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples.

Alternative names

Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10, CGI-102, EXOSC3, RRP40

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR14840(B)

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.

Biological function summary

EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.

Pathways

The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.

Associated diseases and disorders

Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunofluorescent analysis of 4% paraformaldehyde fixed 293T cells labeling EXOSC3 with ab184961 at 1/100 dilution (red). Counterstained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixedHeLa cells labeling EXOSC3 with ab184961 at 1/100 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunoprecipitation analysis of 293T lysates (Lane 1) labeling EXOSC3 with ab184961 at a 1/50 dilution and a negative control PBS (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at a 1500 dilution.

    All lanes: Immunoprecipitation - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Predicted band size: 30 kDa

  • Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    All lanes: Western blot - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961) at 1/10000 dilution

    Lane 1: 293T cell lysates at 20 µg

    Lane 2: HeLa cell lysates at 20 µg

    Lane 3: Jurkat cell lysates at 20 µg

    Lane 4: Raji cell lysates at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 30 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14840(B)] - N-terminal (ab184961)

    Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded Human colon tissue labeling EXOSC3 with ab184961 at 1/50 dilution. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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