Skip to main content

Rabbit Recombinant Monoclonal EXOSC3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (AB251025), expandable thumbnail
  • Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (AB251025), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (AB251025), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (AB251025), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (AB251025), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFIHC-P
Human
Tested
Tested
Expected
Tested
Mouse
Expected
Tested
Tested
Tested
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC3 as peripheral part of the Exo-9 complex stabilizes the hexameric ring of RNase PH-domain subunits through contacts with EXOSC9 and EXOSC5.

Alternative names

RRP40, CGI-102, EXOSC3, Exosome complex component RRP40, Exosome component 3, Ribosomal RNA-processing protein 40, p10

Recommended products

Rabbit Recombinant Monoclonal EXOSC3 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14841
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251025 is the carrier-free version of Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EXOSC3 also known as exosome component 3 is a core part of the RNA exosome complex. This protein weighs approximately 33 kDa and is located in both the nucleus and cytoplasm of eukaryotic cells. EXOSC3 along with other exosome components is highly expressed in tissues with rapid cell division and RNA turnover such as the bone marrow and spleen. These expression patterns are consistent with its function in RNA processing and degradation.

Biological function summary

EXOSC3 plays a critical role in determining RNA stability as part of the exosome complex. This multi-subunit complex degrades various RNA species including mRNA rRNA and snRNA thereby maintaining cellular RNA quality and quantity. EXOSC3 in conjunction with other exosome components executes an important function in RNA surveillance and quality control. Through exosomal complexes EXOSC3 influences many processes that are necessary for cell growth and homeostasis.

Pathways

The exosome component EXOSC3 shows involvement in the RNA processing pathway and the nuclear mRNA catabolic pathway. This protein collaborates with other exosome components like EXOSC10 to perform precise RNA surveillance. The complex carefully regulates RNA processing events within these pathways to ensure cellular homeostasis and proper gene expression regulation.

Associated diseases and disorders

Mutations or malfunctions in EXOSC3 link to specific conditions notably pontocerebellar hypoplasia type 1B and spinal muscular atrophy. In particular through its role in RNA metabolism alterations in EXOSC3's function can lead to motor neuron dysfunction. EXOSC3's correlation with EXOSC8 another exosome component can exacerbate symptoms associated with these neural disorders shedding light on the importance of precise RNA processing in neurological health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.

    Lane 1: Primary: Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, 1/1000 dilution Sample: Lysate of 293T cells immunoprecipated with Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689 at 1/50 Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.

    Lane 2: Primary: Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, 1/1000 dilution. Sample: PBS. Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, 1/1500 dilution.

    All lanes: Immunoprecipitation - Anti-EXOSC3 antibody [EPR14841] - C-terminal (Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689) at 1/1000 dilution

    Lane 1: Lysate of 293T cells immunoprecipated with Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689 at 1/50

    Lane 2: PBS

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

    Predicted band size: 30 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689) at 1/1000 dilution

    Lane 1: Mouse kidney tissue lysate at 10 µg

    Lane 2: Mouse spleen tissue lysate at 10 µg

    Lane 3: Rat kidney tissue lysate at 10 µg

    Lane 4: Rat spleen tissue lysate at 10 µg

    Lane 5: RAW264.7 cell line lysate at 10 µg

    Lane 6: NIH 3T3 cell line lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa

    Observed band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde fixed RAW264.7 cells labeling EXOSC3 with Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689 at 1/250, followed by Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and counterstained with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded mouse kidney tissue labeling EXOSC3 with Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded human ovarian carcinoma tissue labeling EXOSC3 with Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689 at 1/100, followed by prediluted, HRP conjugated secondary antibody and counterstained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689) at 1/1000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025), expandable thumbnail

    Western blot - Anti-EXOSC3 antibody [EPR14841] - BSA and Azide free (ab251025)

    This data was developed using Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-EXOSC3 antibody [EPR14841] - C-terminal (Anti-EXOSC3 antibody [EPR14841] - C-terminal ab190689) at 1/1000 dilution

    All lanes: 293T cell line lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

    Predicted band size: 30 kDa

    Observed band size: 30 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com