Rabbit Polyclonal EXOSC5/CML28 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOSC5 aa 1-200.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human EXOSC5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQT4
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes (PubMed:11782436, PubMed:21269460). In vitro, EXOSC5 does not bind or digest single-stranded RNA and binds to double-stranded DNA without detectable DNase activity (PubMed:20660080).
Alternative names
CML28, RRP46, EXOSC5, Exosome complex component RRP46, Chronic myelogenous leukemia tumor antigen 28, Exosome component 5, Ribosomal RNA-processing protein 46, p12B
Recommended products
Rabbit Polyclonal EXOSC5/CML28 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOSC5 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human EXOSC5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NQT4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EXOSC5 also known as CML28 is a core component of the exosome complex an important multiprotein machinery involved in RNA processing and degradation. EXOSC5 has a molecular mass of approximately 34 kDa and is expressed in a wide range of tissues. It functions as a scaffold within the exosome complex interacting with other exosome subunits to maintain the stability and structure of the complex.
- Biological function summary
EXOSC5 is important for the normal function of the RNA exosome complex which plays a central role in 3'-to-5' RNA processing and degradation. The exosome complex is essential for removing defective RNA molecules and managing RNA stability. EXOSC5 acts as a bridge between the core exosome and additional cofactors that enhance the exosome's specific functions. This regulation ensures appropriate cellular RNA levels impacting gene expression and integrity.
- Pathways
EXOSC5 is involved in the RNA surveillance pathway which monitors and degrades aberrant RNA transcripts in the cell. The pathway ensures that only properly processed RNAs are translated into proteins. Within this framework EXOSC5 interacts with RRP44 a catalytic component of the exosome bolstering its activity in degrading faulty RNAs. The balance of RNA processing and degradation that EXOSC5 supports is vital in maintaining cellular homeostasis.
- Associated diseases and disorders
Abnormalities in EXOSC5 have been linked to autoimmune diseases and cancers. Autoimmune responses may target EXOSC5 leading to disrupted RNA processing. Moreover alterations in EXOSC5 expression or function can contribute to oncogenesis by impacting the cell cycle and apoptosis. In cancers interactions between EXOSC5 and the protein p53 suggest a connection in cell survival pathways highlighting EXOSC5's potential as a therapeutic target in cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunocytochemistry/ Immunofluorescence - Anti-EXOSC5/CML28 antibody (ab154718)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling EXOSC5/CML28 with ab154718 at 1/500 dilution (Green). Alpha-tubulin filaments were labeled red.
Western blot - Anti-EXOSC5/CML28 antibody (ab154718)
All lanes: Western blot - Anti-EXOSC5/CML28 antibody (ab154718) at 1/1000 dilution
Lane 1: HeLa whole cell lysate at 30 µg
Lane 2: HeLa nuclear cell lysate at 30 µg
Predicted band size: 25 kDa
Immunocytochemistry/ Immunofluorescence - Anti-EXOSC5/CML28 antibody (ab154718)
Immunocytochemistry/ Immunofluorescence of HeLa cells staining EXOSC5/CML28 with ab154718 at 1/500. (green)
Phalloidin, a cytoskeleton marker, diluted at 1:100. (red)
Hoechst 33342 staining. (blue)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EXOSC5/CML28 antibody (ab154718)
Immunohistochemical analysis of paraffin-embedded BT483 xenograft labeling EXOSC5/CML28 with ab154718 at 1/500 dilution.
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