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Mouse Monoclonal EXOSC9 antibody. Suitable for WB and reacts with Recombinant fragment samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOSC9.

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Images

Western blot - Anti-EXOSC9 antibody [2337C3a] (AB54283), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Disodium hydrogenorthophosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human EXOSC9. The exact immunogen used to generate this antibody is proprietary information. Database link Q06265

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Recombinant fragment
Tested

Tested
Tested

Species
Recombinant fragment
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.

Alternative names

PMSCL1, EXOSC9, Exosome complex component RRP45, Autoantigen PM/Scl 1, Exosome component 9, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Polymyositis/scleroderma autoantigen 1, Polymyositis/scleroderma autoantigen 75 kDa, PM/Scl-75

Recommended products

Mouse Monoclonal EXOSC9 antibody. Suitable for WB and reacts with Recombinant fragment samples. Immunogen corresponding to Recombinant Fragment Protein within Human EXOSC9.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human EXOSC9. The exact immunogen used to generate this antibody is proprietary information. Database link Q06265
Clone number
2337C3a
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

ab54283 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

EXOSC9 also known as Exosome Component 9 or 3'-5' exoribonuclease CSL4 plays an important mechanical role in RNA processing and degradation. This protein is a component of the exosome complex which is responsible for the controlled breakdown of RNA molecules. The mass of EXOSC9 is approximately 45 kDa. It is expressed in a variety of tissues including brain liver and heart highlighting its importance in diverse cellular activities.

Biological function summary

EXOSC9 functions as a critical element in RNA surveillance and decay pathways within cells. It is a core component of the RNA exosome complex which consists of multiple proteins working together to process and degrade various types of RNA such as rRNA mRNA and small RNAs. The exosome complex plays a significant role in maintaining RNA stability and quality control within eukaryotic cells ensuring the accurate regulation of gene expression.

Pathways

EXOSC9 participates in essential processes involving RNA processing and decay. It is a part of the RNA decay pathway which is vital for the degradation of defective RNA molecules therefore preventing potential cellular dysfunction. EXOSC9 also interacts with proteins like EXOSC10 and EXOSC2 working collectively within the exosome complex to achieve its functionalities in these pathways. This interaction underlines its participation in the larger context of gene expression regulation.

Associated diseases and disorders

Improper functioning of EXOSC9 has been linked to neurodegenerative conditions such as pontocerebellar hypoplasia. Such conditions result from defects in RNA processing and degradation leading to cellular dysregulation. EXOSC9 is also connected to specific cardiovascular disorders due to its involvement in RNA metabolism and stability. Additionally its interaction with other exosome components like EXOSC8 and EXOSC3 further connects it to broader pathways involved in these diseases.

Product promise

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1 product image

  • Western blot - Anti-EXOSC9 antibody [2337C3a] (ab54283), expandable thumbnail

    Western blot - Anti-EXOSC9 antibody [2337C3a] (ab54283)

    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

    All lanes: Western blot - Anti-EXOSC9 antibody [2337C3a] (ab54283)

    All lanes: Recombinant immunising protein

    Predicted band size: 49 kDa

    Observed band size: 42 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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