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AB156686

Anti-EXOSC9 antibody

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(1 Publication)

Rabbit Polyclonal EXOSC9 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human EXOSC9 aa 350 to C-terminus.

View Alternative Names

PMSCL1, EXOSC9, Exosome complex component RRP45, Autoantigen PM/Scl 1, Exosome component 9, P75 polymyositis-scleroderma overlap syndrome-associated autoantigen, Polymyositis/scleroderma autoantigen 1, Polymyositis/scleroderma autoantigen 75 kDa, PM/Scl-75

3 Images
Western blot - Anti-EXOSC9 antibody (AB156686)
  • WB

Unknown

Western blot - Anti-EXOSC9 antibody (AB156686)

All lanes:

Western blot - Anti-EXOSC9 antibody (ab156686) at 0.1 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 49 kDa

true

Exposure time: 10s

Western blot - Anti-EXOSC9 antibody (AB156686)
  • WB

Unknown

Western blot - Anti-EXOSC9 antibody (AB156686)

All lanes:

Western blot - Anti-EXOSC9 antibody (ab156686) at 0.4 µg/mL

Lane 1:

Mouse NIH3T3 whole cell lysate at 50 µg

Lane 2:

Mouse NIH3T3 whole cell lysate at 15 µg

Predicted band size: 49 kDa

true

Exposure time: 3s

Immunoprecipitation - Anti-EXOSC9 antibody (AB156686)
  • IP

Unknown

Immunoprecipitation - Anti-EXOSC9 antibody (AB156686)

Detection of EXOSC9 by Western Blot of Immunoprecipitate.
Immunoprecipitation of 293T whole cell lysate using ab156686 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane) followed by detection 9 with ab156686 at 0.4 μg/ml. The lysate in lane 2 is precipitated with an IgG control antibody. Detection : Chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-EXOSC9 antibody (ab156686)

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human EXOSC9 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q06265

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-10.00000 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "<p></p>" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab156686 was affinity purified using an epitope specific to EXOSC9 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EXOSC9 also known as Exosome Component 9 or 3'-5' exoribonuclease CSL4 plays an important mechanical role in RNA processing and degradation. This protein is a component of the exosome complex which is responsible for the controlled breakdown of RNA molecules. The mass of EXOSC9 is approximately 45 kDa. It is expressed in a variety of tissues including brain liver and heart highlighting its importance in diverse cellular activities.
Biological function summary

EXOSC9 functions as a critical element in RNA surveillance and decay pathways within cells. It is a core component of the RNA exosome complex which consists of multiple proteins working together to process and degrade various types of RNA such as rRNA mRNA and small RNAs. The exosome complex plays a significant role in maintaining RNA stability and quality control within eukaryotic cells ensuring the accurate regulation of gene expression.

Pathways

EXOSC9 participates in essential processes involving RNA processing and decay. It is a part of the RNA decay pathway which is vital for the degradation of defective RNA molecules therefore preventing potential cellular dysfunction. EXOSC9 also interacts with proteins like EXOSC10 and EXOSC2 working collectively within the exosome complex to achieve its functionalities in these pathways. This interaction underlines its participation in the larger context of gene expression regulation.

Improper functioning of EXOSC9 has been linked to neurodegenerative conditions such as pontocerebellar hypoplasia. Such conditions result from defects in RNA processing and degradation leading to cellular dysregulation. EXOSC9 is also connected to specific cardiovascular disorders due to its involvement in RNA metabolism and stability. Additionally its interaction with other exosome components like EXOSC8 and EXOSC3 further connects it to broader pathways involved in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes. EXOSC9 binds to ARE-containing RNAs.
See full target information EXOSC9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of human genetics 102:858-873 PubMed29727687

2018

Variants in EXOSC9 Disrupt the RNA Exosome and Result in Cerebellar Atrophy with Spinal Motor Neuronopathy.

Applications

WB

Species

Human

David T Burns,Sandra Donkervoort,Juliane S Müller,Ellen Knierim,Diana Bharucha-Goebel,Eissa Ali Faqeih,Stephanie K Bell,Abdullah Y AlFaifi,Dorota Monies,Francisca Millan,Kyle Retterer,Sarah Dyack,Sara MacKay,Susanne Morales-Gonzalez,Michele Giunta,Benjamin Munro,Gavin Hudson,Mena Scavina,Laura Baker,Tara C Massini,Monkol Lek,Ying Hu,Daniel Ezzo,Fowzan S AlKuraya,Peter B Kang,Helen Griffin,A Reghan Foley,Markus Schuelke,Rita Horvath,Carsten G Bönnemann
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
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AB54283

Anti-EXOSC9 antibody [2337C3a]

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exosc9-antibody-2337c3a-ab54283

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