AB133237

Anti-Exportin 4/XPO4 antibody [EPR4442(2)]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal Exportin 4/XPO4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

KIAA1721, XPO4, Exportin-4, Exp4

1 Images

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Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (AB133237)

All lanes:

Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (ab133237) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

SW480 cell lysates at 10 µg

Lane 4:

C6 cell lysates at 10 µg

Lane 5:

Raw264.7 cell lysates at 10 µg

Lane 6:

PC12 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 130 kDa

false

Carrier free

Anti-Exportin 4/XPO4 antibody [EPR4442(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4442(2)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exportin 4 also known as XPO4 is a nuclear export receptor part of the karyopherin beta family. It has a molecular weight of approximately 130 kDa. This protein functions by mediating the transport of several cargo molecules from the nucleus to the cytoplasm engaging in the nucleocytoplasmic transport mechanism. Exportin 4 is expressed in various tissues but it shows a higher expression in tissues with active cell division such as the thymus. It specifically recognizes and binds to its cargo proteins in the presence of RanGTP facilitating their export through the nuclear pore complex.

Biological function summary

Exportin 4 plays an important role in the regulation of protein synthesis by exporting molecules involved in translation. It acts as part of a complex with RanGTP a small GTPase that provides the energy required for nuclear transport. This transport process is essential for maintaining cellular function and homeostasis by regulating the localization of proteins that are necessary for cell growth and division. The specificity of the cargo depends on its nuclear export signals which Exportin 4 recognizes with high affinity.

Pathways

Exportin 4 is integrated into the Ran pathway which is vital for nuclear import and export processes. In this pathway Exportin 4 transports proteins necessary for cell cycle progression and gene expression. A related protein in this pathway is RanBP1 which assists in the dissociation of the export complex in the cytoplasm. Exportin 4 also interacts with other proteins like CRM1 to coordinate the export of specific RNA molecules impacting the balance of nuclear and cytoplasmic activities in the cell.

Alterations in Exportin 4 function can influence cancer progression and neurodegenerative diseases. Aberrant export activity can lead to mislocalization of tumor suppressor proteins contributing to oncogenesis. In neurodegenerative conditions such as Alzheimer’s disease Exportin 4 dysregulation affects neuronal function partly due to its interaction with Tau protein which can aggregate abnormally in neurons. Understanding the dynamics of Exportin 4 can therefore provide insights into therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates the nuclear export of proteins (cargos), such as EIF5A, SMAD3 and isoform M2 of PKM (PKM2) (PubMed : 10944119, PubMed : 16449645, PubMed : 26787900). In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins (PubMed : 10944119, PubMed : 16449645). Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor (PubMed : 10944119, PubMed : 16449645). XPO4 then return to the nuclear compartment and mediate another round of transport (PubMed : 10944119, PubMed : 16449645). The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 10944119, PubMed : 16449645). Catalyzes the nuclear export of hypusinated EIF5A; a small cytoplasmic protein that enters nucleus and accumulates within nucleolus if not exported back by XPO4 (PubMed : 10944119). Specifically mediates nuclear export of isoform M2 of PKM (PKM2) following PKM2 deacetylation by SIRT6 (PubMed : 26787900). Also mediates the nuclear import of SOX transcription factors SRY and SOX2 (By similarity).

See full target information XPO4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 11:5755 PubMed33188200

2020

A translational program that suppresses metabolism to shield the genome.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan C Balukoff,J J David Ho,Phaedra R Theodoridis,Miling Wang,Michael Bokros,Lis M Llanio,Jonathan R Krieger,Jonathan H Schatz,Stephen Lee

Journal of cellular biochemistry 121:545-556 PubMed31310365

2019

An evaluation of different Cripto-1 antibodies and their variable results.

Applications

Unspecified application

Species

Unspecified reactive species

Johann Mar Gudbergsson,Meg Duroux

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Exportin 4/XPO4 antibody [EPR4442(2)]

Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (AB133237)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise