AB248416

Anti-Exportin 4/XPO4 antibody [EPR4442(2)] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Exportin 4/XPO4 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA1721, XPO4, Exportin-4, Exp4

1 Images

Unknown

Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] - BSA and Azide free (AB248416)

This data was developed using ab133237, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] (<a href='/en-us/products/primary-antibodies/exportin-4-xpo4-antibody-epr44422-ab133237'>ab133237</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

SW480 cell lysates at 10 µg

Lane 4:

C6 cell lysates at 10 µg

Lane 5:

Raw264.7 cell lysates at 10 µg

Lane 6:

PC12 cell lysates at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 130 kDa

false

Unconjugated

Anti-Exportin 4/XPO4 antibody [EPR4442(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4442(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab248416 is the carrier-free version of ab133237.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exportin 4 also known as XPO4 is a nuclear export receptor part of the karyopherin beta family. It has a molecular weight of approximately 130 kDa. This protein functions by mediating the transport of several cargo molecules from the nucleus to the cytoplasm engaging in the nucleocytoplasmic transport mechanism. Exportin 4 is expressed in various tissues but it shows a higher expression in tissues with active cell division such as the thymus. It specifically recognizes and binds to its cargo proteins in the presence of RanGTP facilitating their export through the nuclear pore complex.

Biological function summary

Exportin 4 plays an important role in the regulation of protein synthesis by exporting molecules involved in translation. It acts as part of a complex with RanGTP a small GTPase that provides the energy required for nuclear transport. This transport process is essential for maintaining cellular function and homeostasis by regulating the localization of proteins that are necessary for cell growth and division. The specificity of the cargo depends on its nuclear export signals which Exportin 4 recognizes with high affinity.

Pathways

Exportin 4 is integrated into the Ran pathway which is vital for nuclear import and export processes. In this pathway Exportin 4 transports proteins necessary for cell cycle progression and gene expression. A related protein in this pathway is RanBP1 which assists in the dissociation of the export complex in the cytoplasm. Exportin 4 also interacts with other proteins like CRM1 to coordinate the export of specific RNA molecules impacting the balance of nuclear and cytoplasmic activities in the cell.

Alterations in Exportin 4 function can influence cancer progression and neurodegenerative diseases. Aberrant export activity can lead to mislocalization of tumor suppressor proteins contributing to oncogenesis. In neurodegenerative conditions such as Alzheimer’s disease Exportin 4 dysregulation affects neuronal function partly due to its interaction with Tau protein which can aggregate abnormally in neurons. Understanding the dynamics of Exportin 4 can therefore provide insights into therapeutic targets for these diseases.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Mediates the nuclear export of proteins (cargos), such as EIF5A, SMAD3 and isoform M2 of PKM (PKM2) (PubMed : 10944119, PubMed : 16449645, PubMed : 26787900). In the nucleus binds cooperatively to its cargo and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins (PubMed : 10944119, PubMed : 16449645). Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor (PubMed : 10944119, PubMed : 16449645). XPO4 then return to the nuclear compartment and mediate another round of transport (PubMed : 10944119, PubMed : 16449645). The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus (PubMed : 10944119, PubMed : 16449645). Catalyzes the nuclear export of hypusinated EIF5A; a small cytoplasmic protein that enters nucleus and accumulates within nucleolus if not exported back by XPO4 (PubMed : 10944119). Specifically mediates nuclear export of isoform M2 of PKM (PKM2) following PKM2 deacetylation by SIRT6 (PubMed : 26787900). Also mediates the nuclear import of SOX transcription factors SRY and SOX2 (By similarity).

See full target information XPO4

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Exportin 4/XPO4 antibody [EPR4442(2)] - BSA and Azide free

Western blot - Anti-Exportin 4/XPO4 antibody [EPR4442(2)] - BSA and Azide free (AB248416)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise