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AB131281

Anti-Exportin-5 antibody [EPR8453]

4

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal Exportin-5 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

KIAA1291, RANBP21, XPO5, Exportin-5, Exp5, Ran-binding protein 21

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Exportin-5 antibody [EPR8453] (AB131281)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Exportin-5 antibody [EPR8453] (AB131281)

Immunofluorescence analysis of A431 cells labelling Exportin-5 with ab131281 at 1/100 dilution.

Western blot - Anti-Exportin-5 antibody [EPR8453] (AB131281)
  • WB

Unknown

Western blot - Anti-Exportin-5 antibody [EPR8453] (AB131281)

All lanes:

Western blot - Anti-Exportin-5 antibody [EPR8453] (ab131281) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 136 kDa,155 kDa,90 kDa

false

OI-RD Scanning - Anti-Exportin-5 antibody [EPR8453] (AB131281)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Exportin-5 antibody [EPR8453] (AB131281)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Exportin-5 antibody [EPR8453] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8453

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Exportin-5 also known as Exp5 and eXportin-5 serves a mechanical role in the cellular export of specific RNA molecules from the nucleus to the cytoplasm. This protein belongs to the karyopherin family and has an approximate molecular mass of about 130 kDa. Exportin-5 primarily expresses in cell types where RNA processing and regulation are actively important though specific distribution patterns vary across different tissues.
Biological function summary

Exportin-5 functions by specifically recognizing and binding to pre-microRNA and small nuclear RNA facilitating their transport out of the nucleus. It operates as a part of a complex with RanGTP which ensures that the RNA cargo is correctly recognized and loaded for transport. This export process is essential for the subsequent RNA maturation and ultimately influences gene expression regulation.

Pathways

While Exportin-5 plays a pivotal role in the RNA transport pathway it is also intricately linked to the microRNA biogenesis pathway. Within these pathways Exportin-5 works closely with RanGTP to mediate the nuclear export of pre-microRNAs setting the stage for their maturation into functional microRNAs in the cytoplasm. It also relates to proteins involved in the RNA-induced silencing complex (RISC) assembly as mature microRNAs are integral parts of RISC.

Exportin-5 appears to be linked to tumorigenesis and certain viral infections. Abnormalities in its function may disrupt microRNA export which can lead to dysregulation of gene expression and contribute to cancer development. Furthermore associations with proteins such as Dicer and RISC-related factors highlight its role in antiviral responses where the microRNA pathway can be modulated by viral mechanisms to evade host defenses.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the nuclear export of proteins bearing a double-stranded RNA binding domain (dsRBD) and double-stranded RNAs (cargos). XPO5 in the nucleus binds cooperatively to the RNA and to the GTPase Ran in its active GTP-bound form. Proteins containing dsRBDs can associate with this trimeric complex through the RNA. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause disassembly of the complex and release of the cargo from the export receptor. XPO5 then returns to the nuclear compartment by diffusion through the nuclear pore complex, to mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Overexpression may in some circumstances enhance RNA-mediated gene silencing (RNAi). Mediates nuclear export of isoform 5 of ADAR/ADAR1 in a RanGTP-dependent manner.. Mediates the nuclear export of micro-RNA precursors, which form short hairpins (PubMed : 14631048, PubMed : 14681208, PubMed : 15613540). Also mediates the nuclear export of synthetic short hairpin RNAs used for RNA interference. In some circumstances can also mediate the nuclear export of deacylated and aminoacylated tRNAs. Specifically recognizes dsRNAs that lack a 5'-overhang in a sequence-independent manner, have only a short 3'-overhang, and that have a double-stranded length of at least 15 base-pairs (PubMed : 19965479). Binding is dependent on Ran-GTP (PubMed : 19965479).. (Microbial infection) Mediates the nuclear export of adenovirus VA1 dsRNA.
See full target information XPO5
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of dermatological science 98:186-194 PubMed32402513

2020

Dysregulation of microRNA expression in diabetic skin.

Applications

Unspecified application

Species

Unspecified reactive species

Enke Baldini,Erika Testa,Christine Voellenkle,Emanuela De Domenico,Francesca Cianfarani,Fabio Martelli,Salvatore Ulisse,Teresa Odorisio

Inflammatory bowel diseases 24:1045-1054 PubMed29688474

2018

Increase in Processing Factors Is Involved in Skewed MicroRNA Expression in Patients with Ulcerative Colitis Who Develop Small Intestine Inflammation after Pouch Surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Hadas Sherman Horev,Keren M Rabinowitz,Hofit Elad,Revital Barkan,Shay Ben-Shachar,Metsada Pasmanik Chor,Iris Dotan

Molecular therapy : the journal of the American So 23:1201-1210 PubMed25896246

2015

Barrier Function of the Repaired Skin Is Disrupted Following Arrest of Dicer in Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Subhadip Ghatak,Yuk Cheung Chan,Savita Khanna,Jaideep Banerjee,Jessica Weist,Sashwati Roy,Chandan K Sen

Cell 155:1568-80 PubMed24360278

2013

Mammalian 5'-capped microRNA precursors that generate a single microRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Mingyi Xie,Mingfeng Li,Anna Vilborg,Nara Lee,Mei-Di Shu,Valeria Yartseva,Nenad Šestan,Joan A Steitz
View all publications
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Product promise

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