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Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185), expandable thumbnail
  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185), expandable thumbnail
  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Expected
Tested
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).

Alternative names

Extracellular matrix protein 1, Secretory component p85, Ecm1

Recommended products

Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22411-279
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

Associated diseases and disorders

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Immunohistochemical analysis of paraffin-embedded Mouse live tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab253185.Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg Mouse lung lysate 10ug with ab253185 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse lung lysate 10ug

    Lane 2: ab253185 IP in Mouse lung lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab253185 in Mouse lung lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds

    All lanes: Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Predicted band size: 61 kDa

    Observed band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab253185.Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg Mouse liver lysate 10ug with ab253185 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse liver lysate 10ug

    Lane 2: ab253185 IP in Mouse liver lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab253185 in Mouse liver lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 min

    All lanes: Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Predicted band size: 61 kDa

    Observed band size: 85 kDa

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    The wild-type and ECM1 knockout mouse liver lysates were kindly provided by an anonymous collaborator.

    ab253185 was shown to specifically react with Extracellular matrix protein 1 in wild-type mouse liver as signal was lost in ECM1 knockout liver. Wild-type and ECM1 knockout samples were subjected to SDS-PAGE. ab253185 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    Exposure time 7.75 secs.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185) at 1/1000 dilution

    Lane 1: Wild-type mouse liver lysate at 20 µg

    Lane 2: ECM1 knockout mouse liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa

    Observed band size: 48 kDa, 85 kDa

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    Mouse ECM1 has two isoforms: A long isoform reported to be 85 kDa, and the shorter form 48 kDa.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 7608209; PMID: 23202415).

    Exposure times: Lanes 1-2: 7.75 seconds; Lane 3: 1 second; Lanes 4-7: 10 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185) at 1/1000 dilution

    Lane 1: 4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: LLC1 (mouse lung carcinoma), whole cell lysate at 20 µg

    Lane 3: Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg

    Lane 4: Mouse skin lysate at 20 µg

    Lane 5: Mouse liver lysate at 20 µg

    Lane 6: Rat skin lysate at 20 µg

    Lane 7: Rat liver lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa

    Observed band size: 48 kDa, 85 kDa

  • Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab253185.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 4T1 (Mouse mammary gland carcinoma epithelial cell) cells labelling Extracellular matrix protein 1 with ab253185 at 1/500 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

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