AB253185

Anti-Extracellular matrix protein 1 antibody [EPR22411-279]

BOND RX™ Validated
RabMAb
Recombinant
KO Validated
What is this?

(1 Review)

(1 Publication)

Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Extracellular matrix protein 1, Secretory component p85, Ecm1

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunohistochemical analysis of paraffin-embedded Mouse live tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling Extracellular matrix protein 1 with ab253185 at 1/5000 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). The section was incubated with ab253185 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer)

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab253185.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 4T1 (Mouse mammary gland carcinoma epithelial cell) cells labelling Extracellular matrix protein 1 with ab253185 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg Mouse liver lysate 10ug with ab253185 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver lysate 10ug
Lane 2 : ab253185 IP in Mouse liver lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253185 in Mouse liver lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 min

All lanes:

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

Predicted band size: 61 kDa

Observed band size: 85 kDa

false

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg Mouse lung lysate 10ug with ab253185 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse lung lysate 10ug
Lane 2 : ab253185 IP in Mouse lung lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253185 in Mouse lung lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185)

Predicted band size: 61 kDa

Observed band size: 85 kDa

false

Unknown

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Mouse ECM1 has two isoforms : A long isoform reported to be 85 kDa, and the shorter form 48 kDa.

The molecular weight observed is consistent with what has been described in the literature (PMID : 7608209; PMID : 23202415).

Exposure times : Lanes 1-2 : 7.75 seconds; Lane 3 : 1 second; Lanes 4-7 : 10 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185) at 1/1000 dilution

Lane 1:

4T1 (mouse mammary gland carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

LLC1 (mouse lung carcinoma), whole cell lysate at 20 µg

Lane 3:

Hepa1-6 (mouse hepatoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

Mouse skin lysate at 20 µg

Lane 5:

Mouse liver lysate at 20 µg

Lane 6:

Rat skin lysate at 20 µg

Lane 7:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 48 kDa,85 kDa

false

Unknown

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

The wild-type and ECM1 knockout mouse liver lysates were kindly provided by an anonymous collaborator.

ab253185 was shown to specifically react with Extracellular matrix protein 1 in wild-type mouse liver as signal was lost in ECM1 knockout liver. Wild-type and ECM1 knockout samples were subjected to SDS-PAGE. ab253185 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.

Exposure time 7.75 secs.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (ab253185) at 1/1000 dilution

Lane 1:

Wild-type mouse liver lysate at 20 µg

Lane 2:

ECM1 knockout mouse liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 48 kDa,85 kDa

false

Carrier free

Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22411-279

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IP, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).

See full target information Ecm1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Renal failure 44:191-203 PubMed35170385

2022

MiR-122-5p promotes peritoneal fibrosis in a rat model of peritoneal dialysis by targeting Smad5 to activate Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yirong Liu,Zhihong Ma,Zhenxing Huang,Dongmei Zou,Junbin Li,Ping Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Extracellular matrix protein 1 antibody [EPR22411-279]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (AB253185)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise