AB267392

Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Carrier free. Suitable for IP, WB, IHC-P, Flow Cyt (Intra) and reacts with Mouse, Rat samples.

View Alternative Names

Extracellular matrix protein 1, Secretory component p85, Ecm1

4 Images

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90%, methanol permeabilized 4T1 (mouse mammary gland carcinoma epithelial cell) cells labeling Extracellular matrix protein 1 with ab253185 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg mouse liver lysate with ab253185 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver lysate 10μg.

Lane 2 : ab253185 IP in mouse liver lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253185 in mouse liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 mins.

All lanes:

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (<a href='/en-us/products/primary-antibodies/extracellular-matrix-protein-1-antibody-epr22411-279-ab253185'>ab253185</a>)

Predicted band size: 61 kDa

Observed band size: 85 kDa

false

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg mouse lung lysate with ab253185 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab253185 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse lung lysate 10μg.

Lane 2 : ab253185 IP in mouse lung lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253185 in mouse lung lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Predicted band size: 61 kDa

Observed band size: 85 kDa

false

Unknown

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab253185).

The wild-type and ECM1 knockout mouse liver lysates were kindly provided by an anonymous collaborator.

ab253185 was shown to specifically react with Extracellular matrix protein 1 in wild-type mouse liver as signal was lost in ECM1 knockout liver. Wild-type and ECM1 knockout samples were subjected to SDS-PAGE. ab253185 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/50,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc™ MP instrument using the ECL technique.

Exposure time 7.75 secs.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] (<a href='/en-us/products/primary-antibodies/extracellular-matrix-protein-1-antibody-epr22411-279-ab253185'>ab253185</a>) at 1/1000 dilution

Lane 1:

Wild-type mouse liver lysate at 20 µg

Lane 2:

ECM1 knockout mouse liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 61 kDa

Observed band size: 48 kDa,85 kDa

false

Unconjugated

Anti-Extracellular matrix protein 1 antibody [EPR22411-279]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22411-279

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P, Flow Cyt (Intra), WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab267392 is the carrier-free version of ab253185.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity (By similarity).

See full target information Ecm1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22411-279] - BSA and Azide free (AB267392)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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