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Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (AB253244), expandable thumbnail
  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (AB253244), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (AB253244), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (AB253244), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBICC/IFIHC-P
Human
Tested
Not recommended
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

Alternative names

Extracellular matrix protein 1, Secretory component p85, ECM1

Recommended products

Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22434-381
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

Associated diseases and disorders

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244), expandable thumbnail

    Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg of MDS-MB-435S (human mammary gland ductal carcinoma melanocyte cell line) whole cell lysate with ab253244 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253244 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: MDS-MB-435S whole cell lysate 10μg (Input).
    Lane 2: ab253244 IP in MDA-MB-435S whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab253244 in MDA-MB-435S whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Predicted band size: 61 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244), expandable thumbnail

    Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure times.

    Lanes 1 & 2: 3 minutes. Lane 3: 37 seconds.

    The expression molecular weight observed is consistent with what has been described in the literature (PMID: 14550953).

    All lanes: Western blot - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244) at 1/1000 dilution

    Lane 1: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 2: MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate at 20 µg

    Lane 3: SK-MEL-28 (human malignant melanoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 61 kDa

    Observed band size: 68 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cervical carcinoma is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (ab253244)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid carcinoma (PMID: 18374945) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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