AB254202

Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Extracellular matrix protein 1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Extracellular matrix protein 1, Secretory component p85, ECM1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human thyroid carcinoma (PMID : 18374945) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253244).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labeling Extracellular matrix protein 1 with ab253244 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cervical carcinoma is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253244).

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Extracellular matrix protein 1 was immunoprecipitated from 0.35 mg of MDS-MB-435S (human mammary gland ductal carcinoma melanocyte cell line) whole cell lysate with ab253244 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab253244 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : MDS-MB-435S whole cell lysate 10μg (Input).
Lane 2 : ab253244 IP in MDA-MB-435S whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab253244 in MDA-MB-435S whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253244).

All lanes:

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] (<a href='/en-us/products/primary-antibodies/extracellular-matrix-protein-1-antibody-epr22434-381-ab253244'>ab253244</a>)

Predicted band size: 61 kDa

Observed band size: 68 kDa

false

Unconjugated

Anti-Extracellular matrix protein 1 antibody [EPR22434-381]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22434-381

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab254202 is the carrier-free version of ab253244.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Extracellular matrix protein 1 often called ECM1 is a matrix protein involved in various mechanical functions in the body. This protein weighs approximately 61 kDa and is widely expressed in human tissues including skin bone lung and brain. ECM1 also goes by the name H3300 vector used in some experimental contexts. Being an extracellular matrix (ECM) component ECM1 plays roles in tissue organization influencing matrix proteins' structural integrity.

Biological function summary

ECM1 contributes to cell-matrix interactions and regulates various cell behaviors such as adhesion proliferation and differentiation. It does this by binding to other ECM proteins and is known not to be part of a specific complex. ECM1 facilitates communication between the cellular environment and matrix thereby supporting the architecture and dynamics of tissues. It modulates angiogenesis linking it with processes like wound healing and vascular development.

Pathways

ECM1 functions within the context of key signaling routes such as the PI3K/AKT pathway known for roles in cell growth and survival. ECM1 interacts with other proteins including matrix metalloproteinases contributing to matrix remodeling activities important for cellular migration and invasion. Cumulatively these interactions highlight ECM1’s place in connective tissue organization and regenerative processes.

ECM1’s dysregulation links it with lipoid proteinosis and skin conditions like Atopic Dermatitis. Imbalances in ECM1 expression or function can lead to abnormal ECM assembly influencing these diseases' development. Moreover abnormalities in ECM1 can alter the behavior of related proteins such as collagen impacting skin integrity and permeability. Understanding ECM1’s role could provide insights into therapeutic strategies for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.

See full target information Extracellular matrix protein 1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immunity 54:1594-1610.e11 PubMed34174183

2021

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Marius Schwabenland,Henrike Salié,Jovan Tanevski,Saskia Killmer,Marilyn Salvat Lago,Alexandra Emilia Schlaak,Lena Mayer,Jakob Matschke,Klaus Püschel,Antonia Fitzek,Benjamin Ondruschka,Henrik E Mei,Tobias Boettler,Christoph Neumann-Haefelin,Maike Hofmann,Angele Breithaupt,Nafiye Genc,Christine Stadelmann,Julio Saez-Rodriguez,Peter Bronsert,Klaus-Peter Knobeloch,Thomas Blank,Robert Thimme,Markus Glatzel,Marco Prinz,Bertram Bengsch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR22434-381] - BSA and Azide free (AB254202)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise