Anti-Extracellular matrix protein 1 antibody [EPR6701]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

Intracellular Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1/20 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

Immunocytochemistry/ Immunofluorescence analysis of A375 (Human malignant melanoma epithelial cell) cells labeling Extracellular matrix protein 1 with purified ab126629 at 1 : 100 dilution (2.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human breast carcinoma tissue by Immunohistochemistry. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126629). Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

ab126629, at 1/100, staining Extracellular matrix protein 1 in formalin fixed, paraffin embedded Human kidney tissue by Immunohistochemistry. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Extracellular matrix protein 1 with purified ab126629 at 1 : 100 dilution (2.01 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IP

Unknown

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

ab126629 (purified) at 1 : 20 dilution (2μg) immunoprecipitating Extracellular matrix protein 1 in A375 whole cell lysate.
Lane 1 (input) : A375 (Human malignant melanoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab126629 & A375 whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab126629 in A375 whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629)

Predicted band size: 61 kDa,78 kDa

Observed band size: 60 kDa

false

• WB

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Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

All lanes:

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/1000 dilution

Lane 1:

MDA MB 435 cell line lysate at 10 µg

Lane 2:

Human skin cell lysate at 10 µg

Lane 3:

A375 cell line lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibodty at 1/2000 dilution

Predicted band size: 61 kDa

false

• WB

Lab

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

All lanes:

Western blot - Anti-Extracellular matrix protein 1 antibody [EPR6701] (ab126629) at 1/2000 dilution

Lane 1:

A375 (Human malignant melanoma epithelial cell) at 15 µg

Lane 2:

Rat heart lysates at 15 µg

Lane 3:

Rat kidney lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 61 kDa

Observed band size: 61 kDa

false

• OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Extracellular matrix protein 1 antibody [EPR6701] (AB126629)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.