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AB194448

Anti-EYA1 antibody

5

(1 Review)

|

(2 Publications)

Mouse Polyclonal EYA1 antibody. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EYA1 aa 100 to C-terminus.

View Alternative Names

Protein phosphatase EYA1, Eyes absent homolog 1, EYA1

2 Images
Western blot - Anti-EYA1 antibody (AB194448)
  • WB

Supplier Data

Western blot - Anti-EYA1 antibody (AB194448)

The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.

All lanes:

Western blot - Anti-EYA1 antibody (ab194448) at 1/1000 dilution

All lanes:

EYA1 recombinant protein (immunizing peptide) at 0.2 µg

Predicted band size: 65 kDa

Observed band size: 34 kDa

false

Western blot - Anti-EYA1 antibody (AB194448)
  • WB

Supplier Data

Western blot - Anti-EYA1 antibody (AB194448)

All lanes:

Western blot - Anti-EYA1 antibody (ab194448) at 1/500 dilution

All lanes:

Jurkat cell lysate at 50 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human EYA1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99502

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EYA1 protein known as Eyes Absent Homolog 1 acts as a transcriptional coactivator and tyrosine phosphatase. It has a molecular weight of approximately 65 kDa. EYA1 expresses in various tissues including the kidney eye and branchial arches during developmental stages. It plays essential roles in organogenesis by modulating gene expression via its interaction with other proteins.
Biological function summary

EYA1 contributes significantly to the formation of several organs during embryonic development. This protein is part of the EYA-SIX transcriptional complex and works closely with SIX family proteins to regulate the expression of multiple key developmental genes. Through its phosphatase activity EYA1 also affects phosphorylated protein substrates altering their function and stability.

Pathways

EYA1 plays an important role in the regulation of the WNT and SHH signaling pathways both of which are fundamental for embryonic development and cell differentiation. EYA1 interacts with related proteins like SIX1 aiding in the transcriptional regulation needed for cellular proliferation and differentiation. Interferences in these pathways can lead to developmental anomalies.

Mutations or dysregulation of EYA1 have links to conditions such as Branchio-Oto-Renal (BOR) syndrome and congenital cataracts. The association with BOR syndrome arises from EYA1's interaction with other related proteins including SIX1 that regulate the development of the ear kidney and certain facial structures. EYA1's role in ocular development also connects it to certain types of hereditary cataracts demonstrating its impact on normal physiological processes when mutated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (By similarity). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed : 19234442). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis (By similarity). Also has phosphatase activity with proteins phosphorylated on Ser and Thr residues (in vitro) (By similarity). Required for normal embryonic development of the craniofacial and trunk skeleton, kidneys and ears (By similarity). Together with SIX1, it plays an important role in hypaxial muscle development; in this it is functionally redundant with EYA2 (By similarity).
See full target information EYA1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 25:10980-10989 PubMed34773364

2021

The deubiquitinase OTUB1 fosters papillary thyroid carcinoma growth through EYA1 stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Peiyi Xie,Qing Chao,Jiuang Mao,Yue Liu,Jiayu Fang,Jing Xie,Jing Zhen,Yongqi Ding,Bidong Fu,Yun Ke,Da Huang

Molecular medicine reports 22:1767-1774 PubMed32705258

2020

Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Bian,Qiu Li,Qiaolian Li,Ruigen Pan
View all publications
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Product promise

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