AB92505

Anti-EYA2 antibody [EPR2942]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal EYA2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

EAB1, EYA2, Protein phosphatase EYA2, Eyes absent homolog 2

1 Images

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Western blot - Anti-EYA2 antibody [EPR2942] (AB92505)

All lanes:

Western blot - Anti-EYA2 antibody [EPR2942] (ab92505) at 1/50000 dilution

Lane 1:

Y-79 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP-labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 59 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2942

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50000 - 1/200000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The EYA2 protein also known as Eyes Absent Homolog 2 is part of the EYA family of proteins. It has a mass of around 67 kDa. EYA2 functions as a transcriptional coactivator and phosphatase contributing to gene expression regulation. The protein is typically expressed in tissues including the brain kidney and ovaries. EYA2’s phosphatase activity is important in dephosphorylating tyrosine residues on target proteins influencing their function and stability.

Biological function summary

EYA2 engages in critical developmental processes and cellular stress responses. It functions as part of a phosphatase complex with the SIX family of proteins where it plays a role in the regulation of eye development and other organogenesis processes. EYA2's involvement extends to DNA repair as it modulates the activity of proteins engaged in maintaining genomic integrity. This role is essential for normal cellular function and response to environmental stressors.

Pathways

EYA2 participates in the hippo signaling pathway and the DNA damage repair pathway. Through the hippo signaling pathway EYA2 interacts with several other proteins including YAP and TAZ influencing cell proliferation and apoptosis. In the DNA damage repair pathway it associates with proteins such as ATR and ATM ensuring effective repair of DNA lesions and maintenance of genomic stability.

EYA2 is connected to various cancers including breast and ovarian cancer. Alterations in EYA2 expression or activity can lead to unchecked cell proliferation and tumor development. Cancer metastasis often shows increased levels of EYA2 suggesting a role in cancer progression. Furthermore EYA2's interaction with proteins like SIX1 contributes to oncogenic processes highlighting potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions both as protein phosphatase and as transcriptional coactivator for SIX1, and probably also for SIX2, SIX4 and SIX5 (PubMed : 12500905, PubMed : 23435380). Tyrosine phosphatase that dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) and promotes efficient DNA repair via the recruitment of DNA repair complexes containing MDC1. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress (PubMed : 19351884). Its function as histone phosphatase may contribute to its function in transcription regulation during organogenesis. Plays an important role in hypaxial muscle development together with SIX1 and DACH2; in this it is functionally redundant with EYA1 (PubMed : 12500905).

See full target information EYA2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 13:62-71 PubMed24196439

2013

CDK6 binds and promotes the degradation of the EYA2 protein.

Applications

IP, WB

Species

Unspecified reactive species, Unspecified reactive species

Dawn Kohrt,Jennifer Crary,Marc Zimmer,Aaron N Patrick,Heide L Ford,Philip W Hinds,Martha J Grossel

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EYA2 antibody [EPR2942]

Western blot - Anti-EYA2 antibody [EPR2942] (AB92505)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise