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AB251675

Anti-EYA4 antibody

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(3 Publications)

Rabbit Polyclonal EYA4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human EYA4 aa 1-150.

View Alternative Names

Protein phosphatase EYA4, Eyes absent homolog 4, EYA4

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)

Paraffin-embedded human kidney tissue stained for EYA4 using ab251675 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)

Paraffin-embedded human skeletal muscle tissue stained for EYA4 using ab251675 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-EYA4 antibody (AB251675)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EYA4 antibody (AB251675)

PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EYA4 (green) using ab251675 at 4 µg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)

Paraffin-embedded human fallopian tube tissue stained for EYA4 using ab251675 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EYA4 antibody (AB251675)

Paraffin-embedded human prostate tissue stained for EYA4 using ab251675 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-EYA4 antibody (AB251675)
  • WB

Supplier Data

Western blot - Anti-EYA4 antibody (AB251675)

All lanes:

Western blot - Anti-EYA4 antibody (ab251675)

All lanes:

Human fallopian tube tissue lysate

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EYA4 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O95677

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EYA4 also known as Eyes Absent 4 is a protein involved in several important cellular processes. It has an approximate molecular mass of 64 kDa. This protein acts as a phosphatase removing phosphate groups from specific proteins. EYA4 is expressed in various tissues including the heart brain and kidneys. Its presence in these diverse tissues indicates its importance in multiple cellular functions.
Biological function summary

EYA4 contributes to the DNA damage repair process and is important for normal cellular development and function. The protein exists as part of a transcriptional complex often interacting with other proteins to regulate gene expression. This interaction suggests a role in cellular differentiation and proliferation supporting healthy developmental processes and maintaining cell health over time.

Pathways

EYA4 plays an integral role in the Notch signaling and DNA repair pathways. In the Notch signaling pathway it associates with the transcription co-activator protein YAP to influence cell fate decisions. This involvement highlights its importance in cellular architecture maintenance. Its function in the DNA repair pathway further links it with the ATM protein coordinating effective responses to genomic stress and preserving genomic integrity.

Alterations in EYA4 expression or function can contribute to sensorineural hearing loss and certain types of cancer including breast cancer. Defective EYA4 interactions with the protein SIX1 have been linked to hearing impairments. In cancer biology aberrant EYA4 function may disrupt its DNA repair capability facilitating genomic instability and tumor progression indicating a critical role in maintaining cellular homeostasis and preventing disease.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye (By similarity).
See full target information EYA4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer medicine 13:e7471 PubMed39015025

2024

RGS1 and CREB5 are direct and common transcriptional targets of ZNF384-fusion proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chiharu Yamada,Kentaro Okada,Koya Odaira,Mahiru Tokoro,Eisuke Iwamoto,Masashi Sanada,Mina Noura,Syuichi Okamoto,Takahiko Yasuda,Shinobu Tsuzuki,Hitoshi Kiyoi,Fumihiko Hayakawa

Neoplasia (New York, N.Y.) 44:100931 PubMed37647805

2023

Histone H3 K27M-mediated regulation of cancer cell stemness and differentiation in diffuse midline glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Monika Sharma,Ivana Barravecchia,Brian Magnuson,Sarah F Ferris,April Apfelbaum,Nneka E Mbah,Jeanette Cruz,Varunkumar Krishnamoorthy,Robert Teis,McKenzie Kauss,Carl Koschmann,Costas A Lyssiotis,Mats Ljungman,Stefanie Galban

Saudi journal of gastroenterology : official journal of the Saudi Gastroenterology Association 28:456-465 PubMed36453428

2022

Aberrant DNA methylation and expression of in gastric cardia intestinal metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxi Li,Zhaohui Liu,Guohua Xu,Shibin Wu,Yunhui Peng,Ruinuan Wu,Shukun Zhao,Xiaoqi Liao,Runhua Lin
View all publications
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Product promise

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