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AB263961

Anti-EZH1 antibody

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(2 Publications)

Rabbit Polyclonal EZH1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1.

View Alternative Names

KIAA0388, EZH1, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1

1 Images
Western blot - Anti-EZH1 antibody (AB263961)
  • WB

Supplier Data

Western blot - Anti-EZH1 antibody (AB263961)

All lanes:

Western blot - Anti-EZH1 antibody (ab263961) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 nuclear extract at 10 µg

Secondary

All lanes:

Peroxidase rabbit secondary antibody for 60 min at RT at 1/70000 dilution

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human EZH1. The exact immunogen used to generate this antibody is proprietary information.

Q92800

Specificity

A BLAST analysis was used to suggest reactivity with this protein in mouse, bovine, and orangutan species based on 100% homology for the immunogen sequence.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Anti-EZH1 is directed against human EZH1 at an internal position. This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EZH1 also known as Enhancer of Zeste Homolog 1 functions as a histone-lysine N-methyltransferase. This enzyme plays a role in transcriptional repression through histone methylation specifically the trimethylation of histone H3 at lysine 27 (H3K27me3). The mass of EZH1 is approximately 85 kDa. Expression of EZH1 spans various tissues with notable presence in embryonic stem cells and adult tissues.
Biological function summary

The protein EZH1 participates in the formation of the Polycomb Repressive Complex 2 (PRC2). Within this complex EZH1 collaborates with other proteins to regulate gene expression epigenetically. PRC2 activity influences cell identity and development by maintaining the silenced state of specific genes. The presence of EZH1 in this complex indicates its significance in modulating developmental pathways and cellular differentiation processes.

Pathways

EZH1 contributes to epigenetic regulation and is involved in the Wnt signaling pathway. This enzyme's action influences cell cycle regulation and stem cell pluripotency. Within these pathways EZH1 is functionally related to proteins like EZH2 which shares a similar role in histone methylation. The interaction of EZH1 with these pathways highlights its influence on maintaining genetic expression patterns through cell division and differentiation.

EZH1 shows a connection to cancer and developmental disorders. Alterations in EZH1 expression or function can contribute to the development of certain cancers including prostate cancer through its interaction with EZH2. Additionally dysregulation of EZH1-related epigenetic pathways can lead to developmental disorders such as Weaver syndrome. This links EZH1 to critical regulatory roles in both normal development and disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
See full target information EZH1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Clinics (Sao Paulo, Brazil) 80:100550 PubMed39667201

2024

CircRBM33 competitively binds miR-15a-5p to mediate EZH1 expression to ameliorate sepsis-induced acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinquan Lin,Qiongying Wei,Zhipeng Fang

Scientific reports 13:5374 PubMed37005468

2023

Spatial subcellular organelle networks in single cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mythreye Venkatesan,Nicholas Zhang,Benoit Marteau,Yukina Yajima,Nerea Ortiz De Zarate Garcia,Zhou Fang,Thomas Hu,Shuangyi Cai,Adam Ford,Harrison Olszewski,Andrew Borst,Ahmet F Coskun
View all publications
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Product promise

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