Rabbit Polyclonal EZH1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1 aa 1-50.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Or Tris-EDTA buffer pH8.0 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
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Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.
KIAA0388, EZH1, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1
Rabbit Polyclonal EZH1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1 aa 1-50.
IgG
Rabbit
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
EZH1 also known as Enhancer of Zeste Homolog 1 functions as a histone-lysine N-methyltransferase. This enzyme plays a role in transcriptional repression through histone methylation specifically the trimethylation of histone H3 at lysine 27 (H3K27me3). The mass of EZH1 is approximately 85 kDa. Expression of EZH1 spans various tissues with notable presence in embryonic stem cells and adult tissues.
The protein EZH1 participates in the formation of the Polycomb Repressive Complex 2 (PRC2). Within this complex EZH1 collaborates with other proteins to regulate gene expression epigenetically. PRC2 activity influences cell identity and development by maintaining the silenced state of specific genes. The presence of EZH1 in this complex indicates its significance in modulating developmental pathways and cellular differentiation processes.
EZH1 contributes to epigenetic regulation and is involved in the Wnt signaling pathway. This enzyme's action influences cell cycle regulation and stem cell pluripotency. Within these pathways EZH1 is functionally related to proteins like EZH2 which shares a similar role in histone methylation. The interaction of EZH1 with these pathways highlights its influence on maintaining genetic expression patterns through cell division and differentiation.
EZH1 shows a connection to cancer and developmental disorders. Alterations in EZH1 expression or function can contribute to the development of certain cancers including prostate cancer through its interaction with EZH2. Additionally dysregulation of EZH1-related epigenetic pathways can lead to developmental disorders such as Weaver syndrome. This links EZH1 to critical regulatory roles in both normal development and disease pathology.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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7.5% SDS PAGE.
Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)
All lanes: Western blot - Anti-EZH1 antibody - N-terminal (ab137693) at 1/1000 dilution
All lanes: whole cell lysate prepared from HepG2 cells at 30 µg
Predicted band size: 85 kDa
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab137693 at 1/1000 dilution.
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ab137693 at 1/500 dilution.
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