AB137693

Anti-EZH1 antibody - N-terminal

Name: Anti-EZH1 antibody - N-terminal (ab137693) | Abcam
Brand: Abcam Limited
SKU: AB137693
Rating: 1 (2 reviews)

(2 Reviews)

(2 Publications)

Rabbit Polyclonal EZH1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1 aa 1-50.

View Alternative Names

KIAA0388, EZH1, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-EZH1 antibody - N-terminal (AB137693)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-EZH1 antibody - N-terminal (AB137693)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab137693 at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EZH1 antibody - N-terminal (AB137693)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EZH1 antibody - N-terminal (AB137693)

Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using ab137693 at 1/500 dilution.

Unknown

Western blot - Anti-EZH1 antibody - N-terminal (AB137693)

7.5% SDS PAGE.

Secondary antibody - anti-rabbit HRP (ab6721)

All lanes:

Western blot - Anti-EZH1 antibody - N-terminal (ab137693) at 1/1000 dilution

All lanes:

whole cell lysate prepared from HepG2 cells at 30 µg

Predicted band size: 85 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human EZH1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q92800

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "Or Tris-EDTA buffer pH8.0 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EZH1 also known as Enhancer of Zeste Homolog 1 functions as a histone-lysine N-methyltransferase. This enzyme plays a role in transcriptional repression through histone methylation specifically the trimethylation of histone H3 at lysine 27 (H3K27me3). The mass of EZH1 is approximately 85 kDa. Expression of EZH1 spans various tissues with notable presence in embryonic stem cells and adult tissues.

Biological function summary

The protein EZH1 participates in the formation of the Polycomb Repressive Complex 2 (PRC2). Within this complex EZH1 collaborates with other proteins to regulate gene expression epigenetically. PRC2 activity influences cell identity and development by maintaining the silenced state of specific genes. The presence of EZH1 in this complex indicates its significance in modulating developmental pathways and cellular differentiation processes.

Pathways

EZH1 contributes to epigenetic regulation and is involved in the Wnt signaling pathway. This enzyme's action influences cell cycle regulation and stem cell pluripotency. Within these pathways EZH1 is functionally related to proteins like EZH2 which shares a similar role in histone methylation. The interaction of EZH1 with these pathways highlights its influence on maintaining genetic expression patterns through cell division and differentiation.

EZH1 shows a connection to cancer and developmental disorders. Alterations in EZH1 expression or function can contribute to the development of certain cancers including prostate cancer through its interaction with EZH2. Additionally dysregulation of EZH1-related epigenetic pathways can lead to developmental disorders such as Weaver syndrome. This links EZH1 to critical regulatory roles in both normal development and disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

See full target information EZH1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Biomedicines 9: PubMed34944658

2021

Divergent Effects of EZH1 and EZH2 Protein Expression on the Prognosis of Patients with T-Cell Lymphomas.

Applications

Unspecified application

Species

Unspecified reactive species

Franziska Lea Schümann,Elisabeth Groß,Marcus Bauer,Christian Rohde,Sarah Sandmann,Denis Terziev,Lutz P Müller,Guido Posern,Andreas Wienke,Falko Fend,Martin-Leo Hansmann,Wolfram Klapper,Andreas Rosenwald,Harald Stein,Martin Dugas,Carsten Müller-Tidow,Claudia Wickenhauser,Mascha Binder,Thomas Weber

Journal of drug targeting 25:125-131 PubMed27633093

2016

miR-17-5p down-regulation contributes to erlotinib resistance in non-small cell lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Weihua Zhang,Jun Lin,Peng Wang,Jian Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-EZH1 antibody - N-terminal

Immunocytochemistry/ Immunofluorescence - Anti-EZH1 antibody - N-terminal (AB137693)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EZH1 antibody - N-terminal (AB137693)

Western blot - Anti-EZH1 antibody - N-terminal (AB137693)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise