AB176115

Anti-EZH1 antibody - N-terminal

Name: Anti-EZH1 antibody - N-terminal (ab176115) | Abcam
Brand: Abcam Limited
SKU: AB176115
Price: 525 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal EZH1 antibody. N-terminal. Suitable for WB and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human EZH1 aa 150-250.

View Alternative Names

KIAA0388, EZH1, Histone-lysine N-methyltransferase EZH1, ENX-2, Enhancer of zeste homolog 1

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Supplier Data

Western blot - Anti-EZH1 antibody - N-terminal (AB176115)

Lane 1:

Western blot - Anti-EZH1 antibody - N-terminal (ab176115) at 1 µg/mL

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Western blot - Anti-EZH1 antibody - N-terminal (ab176115) at 2 µg/mL

All lanes:

mouse lung tissue lysate at 15 µg

Predicted band size: 85 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

Synthetic Peptide within Human EZH1 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q92800

Specificity

Multiple isoforms of EZH1 are known to exist. EZH1 ab176115 is predicted to not cross-react with EZH2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176115 is affinity chromatography purified via peptide column.

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

EZH1 also known as Enhancer of Zeste Homolog 1 functions as a histone-lysine N-methyltransferase. This enzyme plays a role in transcriptional repression through histone methylation specifically the trimethylation of histone H3 at lysine 27 (H3K27me3). The mass of EZH1 is approximately 85 kDa. Expression of EZH1 spans various tissues with notable presence in embryonic stem cells and adult tissues.

Biological function summary

The protein EZH1 participates in the formation of the Polycomb Repressive Complex 2 (PRC2). Within this complex EZH1 collaborates with other proteins to regulate gene expression epigenetically. PRC2 activity influences cell identity and development by maintaining the silenced state of specific genes. The presence of EZH1 in this complex indicates its significance in modulating developmental pathways and cellular differentiation processes.

Pathways

EZH1 contributes to epigenetic regulation and is involved in the Wnt signaling pathway. This enzyme's action influences cell cycle regulation and stem cell pluripotency. Within these pathways EZH1 is functionally related to proteins like EZH2 which shares a similar role in histone methylation. The interaction of EZH1 with these pathways highlights its influence on maintaining genetic expression patterns through cell division and differentiation.

EZH1 shows a connection to cancer and developmental disorders. Alterations in EZH1 expression or function can contribute to the development of certain cancers including prostate cancer through its interaction with EZH2. Additionally dysregulation of EZH1-related epigenetic pathways can lead to developmental disorders such as Weaver syndrome. This links EZH1 to critical regulatory roles in both normal development and disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH1 complex, which methylates 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. Able to mono-, di- and trimethylate 'Lys-27' of histone H3 to form H3K27me1, H3K27me2 and H3K27me3, respectively. Required for embryonic stem cell derivation and self-renewal, suggesting that it is involved in safeguarding embryonic stem cell identity. Compared to EZH2-containing complexes, it is less abundant in embryonic stem cells, has weak methyltransferase activity and plays a less critical role in forming H3K27me3, which is required for embryonic stem cell identity and proper differentiation.

See full target information EZH1

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:4513-4531 PubMed35864958

2022

Maternal EHMT2 is essential for homologous chromosome segregation by regulating transcription in oocyte meiosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Gang Meng,Wen-Long Lei,Xukun Lu,Xiao-Yu Liu,Xue-Shan Ma,Xiao-Qing Nie,Zheng-Hui Zhao,Qian-Nan Li,Lin Huang,Yi Hou,Ying-Chun Ouyang,Lei Li,Tie-Shan Tang,Heide Schatten,Wei Xie,Shao-Rong Gao,Xiang-Hong Ou,Zhen-Bo Wang,Qing-Yuan Sun

ACS pharmacology & translational science 5:491-507 PubMed35837138

2022

Targeting Triple-Negative Breast Cancer by a Novel Proteolysis Targeting Chimera Degrader of Enhancer of Zeste Homolog 2.

Applications

Unspecified application

Species

Unspecified reactive species

Brandon Dale,Chris Anderson,Kwang-Su Park,H Ümit Kaniskan,Anqi Ma,Yudao Shen,Chengwei Zhang,Ling Xie,Xian Chen,Xufen Yu,Jian Jin

Nature communications 11:6354 PubMed33311485

2020

PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome.

Applications

Unspecified application

Species

Unspecified reactive species

Tie-Gang Meng,Qian Zhou,Xue-Shan Ma,Xiao-Yu Liu,Qing-Ren Meng,Xian-Ju Huang,Hong-Lin Liu,Wen-Long Lei,Zheng-Hui Zhao,Ying-Chun Ouyang,Yi Hou,Heide Schatten,Xiang-Hong Ou,Zhen-Bo Wang,Shao-Rong Gao,Qing-Yuan Sun

Cell stem cell 24:769-784.e6 PubMed30905620

2019

Mesenchymal Niche-Specific Expression of Cxcl12 Controls Quiescence of Treatment-Resistant Leukemia Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Puneet Agarwal,Stephan Isringhausen,Hui Li,Andrew J Paterson,Jianbo He,Álvaro Gomariz,Takashi Nagasawa,César Nombela-Arrieta,Ravi Bhatia

Nature neuroscience 16:1745-53 PubMed24162653

2013

EZH2-mediated H3K27 trimethylation mediates neurodegeneration in ataxia-telangiectasia.

Applications

Unspecified application

Species

Unspecified reactive species

Jiali Li,Ronald P Hart,Elyse M Mallimo,Mavis R Swerdel,Alexander W Kusnecov,Karl Herrup

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-EZH1 antibody - N-terminal

Western blot - Anti-EZH1 antibody - N-terminal (AB176115)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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