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AB204954

Anti-Ezrin antibody [6F1C3]

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(1 Publication)

Mouse Monoclonal Ezrin antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human EZR aa 250-500.

View Alternative Names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

5 Images
Flow Cytometry - Anti-Ezrin antibody [6F1C3] (AB204954)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Ezrin antibody [6F1C3] (AB204954)

Flow cytometric analysis of A431 cells labeling Ezrin with ab204954 at 1/200 (green) or negative control (red)

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1C3] (AB204954)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [6F1C3] (AB204954)

Immunofluorescent analysis of Hela cells labeling Ezrin with ab204954 at 1/200 (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

Lane 1:

Jurkat cell lysate

Lane 2:

A431 cell lysate

Lane 3:

MCF-7 cell lysate

Lane 4:

HeLa cell lysate

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

(Expected MW is 47.1 kDa)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

All lanes:

Human Ezrin (aa: 292-464) recombinant protein.

Predicted band size: 69 kDa

false

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)
  • WB

Supplier Data

Western blot - Anti-Ezrin antibody [6F1C3] (AB204954)

All lanes:

Western blot - Anti-Ezrin antibody [6F1C3] (ab204954) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

Ezrin (AA: 292-464)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 69 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6F1C3

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human EZR aa 250-500. The exact immunogen used to generate this antibody is proprietary information.

P15311

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Structure (London, England : 1993) 26:446-458.e8 PubMed29478821

2018

Disease Variants of FGFR3 Reveal Molecular Basis for the Recognition and Additional Roles for Cdc37 in Hsp90 Chaperone System.

Applications

Unspecified application

Species

Unspecified reactive species

Tom D Bunney,Alison J Inglis,Domenico Sanfelice,Brendan Farrell,Christopher J Kerr,Gary S Thompson,Glenn R Masson,Nethaji Thiyagarajan,Dmitri I Svergun,Roger L Williams,Alexander L Breeze,Matilda Katan
View all publications
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Product promise

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