Anti-Ezrin antibody [EP886Y] - BSA and Azide free

14 product images

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  Western blot - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
  Lane 2: Ezrin knockout HAP1 whole cell lysate (20 μg)
  Lane 3: HeLa whole cell lysate (20 μg)
  Lane 4: HCT116 whole cell lysate (20 μg)
  

  Lanes 1 - 4: Merged signal (red and green). Green - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 observed at 75 kDa. Red - loading control, ab18058, observed at 130 kDa.

  Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 was shown to specifically react with Ezrin in wild-type HAP1 cells as signal was lost in Ezrin knockout cells. Wild-type and Ezrin knockout samples were subjected to SDS-PAGE. Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  This data was developed using the same antibody clone in a different buffer formulation containing bsa and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

  All lanes: Western blot - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839)

  Predicted band size: 69 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  This image was made using Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 which is the same antibody as ab239832 with BSA and Azide
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling Ezrin with Purified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at 1:250 dilution (3.28 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

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  Immunoprecipitation - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  This image was made using Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 which is the same antibody as ab239832 with BSA and Azide
  Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 (purified) at 1:40 dilution (2μg) immunoprecipitating Ezrin in HeLa whole cell lysate.
  Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
  Lane 2 (+): Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 & HeLa whole cell lysate
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 in HeLa whole cell lysate
  For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
  Blocking and diluting buffer: 5% NFDM/TBST.

  All lanes: Immunoprecipitation - Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839)

  Predicted band size: 69 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  This image was made using Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 which is the same antibody as ab239832 with BSA and Azide
  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder carcinoma tissue sections labeling Ezrin with Purified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at 1:250 dilution (3.28 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.

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  Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  This image was made using Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 which is the same antibody as ab239832 with BSA and Azide
  Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ezrin with Purified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at 1:500 dilution (1.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

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  Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  This image was made using Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 which is the same antibody as ab239832 with BSA and Azide Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Ezrin with Purified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at 1/800 dilution (1 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

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  Flow Cytometry (Intracellular) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Overlay histogram showing SH-SY5Y cells stained with Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).
  

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  Immunocytochemistry/ Immunofluorescence - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  ICC/IF image of unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 at a 1:100 dilution staining Ezrin in human colon carcinoma tissue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Fluorescent immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839. Green-Ezrin red-PI
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 showing positive staining in Breast carcinoma tissue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 showing positive staining in Prostatic carcinoma tissue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 showing positive staining in Hepatocellular carcinoma tissue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (ab239832)

  Unpurified Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839 showing positive staining in Normal kidney tissue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Ezrin antibody [EP886Y] - Plasma Membrane Marker ab40839).