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AB75840

Anti-Ezrin antibody [EP924Y]

  • 20ul selling size
  • RabMAb
  • KO Validated
  • Recombinant
  • What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal Ezrin antibody. Suitable for WB and reacts with Human, Rat, Mouse samples. Cited in 6 publications.

View Alternative Names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

1 Images
Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)
  • WB

Lab

Western blot - Anti-Ezrin antibody [EP924Y] (AB75840)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time :
Lane 1 : 20 seconds;
Lane 2 and Lane 3 : 180 seconds.

All lanes:

Western blot - Anti-Ezrin antibody [EP924Y] (ab75840) at 1/2000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

Lane 2:

Mouse brain lysate at 15 µg

Lane 3:

Rat brain lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

false

  • Carrier free

    Anti-Ezrin antibody [EP924Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP924Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>ab75840 detects both phosphorylated and non phosphorylated Ezrin.</p>

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Alternative Products

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Primary Antibodies

AB198522

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  • 0
Primary Antibodies

AB40839

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Primary Antibodies

AB52916

Anti-Ezrin (phospho Y353) antibody [EP925Y]

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Primary Antibodies

AB198520

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  • 0
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Primary Antibodies

AB204954

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Primary Antibodies

AB239832

Anti-Ezrin antibody [EP886Y] - BSA and Azide free

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin antibody [EP886Y] - BSA and Azide free (AB239832)

  • 0
  • 0

Complementary Products

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AB278116

Human Ezrin ELISA Kit

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AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Production method for this product has been changed from hybridoma to recombinant on 15th August 2024

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39125907

2024

Proteomic Analysis Reveals Physiological Activities of Aβ Peptide for Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Ai,Zeyu Cao,Zhaoru Ma,Qinghuan Liu,Wei Huang,Taolei Sun,Jing Li,Chenxi Yang

Experimental and therapeutic medicine 21:112 PubMed33335575

2020

Increased expression of IFN-γ in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Huiqiang Liu,Wenhao Wang,Chongdong Liu

Experimental and therapeutic medicine 17:2495-2502 PubMed30906437

2019

Role of mTOR signaling in the regulation of high glucose-induced podocyte injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuyue Li,Yan Zeng,Qing Jiang,Cong Wu,Jing Zhou

Translational oncology 11:1-10 PubMed29132012

2017

Distinct Ezrin Truncations Differentiate Metastases in Sentinel Lymph Nodes from Unaffected Lymph Node Tissues, from Primary Breast Tumors, and from Healthy Glandular Breast Tissues.

Applications

WB

Species

Human

Claudia Röwer,Christian George,Toralf Reimer,Bernd Stengel,Anngret Radtke,Bernd Gerber,Michael O Glocker

Reproductive biology and endocrinology : RB&E 7:95 PubMed19737422

2009

Participation of ezrin in bacterial uptake by trophoblast giant cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kenta Watanabe,Masato Tachibana,Suk Kim,Masahisa Watarai

FEBS letters 582:3663-8 PubMed18840437

2008

MicroRNA-183 regulates Ezrin expression in lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guofu Wang,Weimin Mao,Shu Zheng
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com