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AB47293

Anti-Ezrin (phospho T567) antibody

5

(3 Reviews)

|

(20 Publications)

Rabbit Polyclonal Ezrin phospho T567 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 20 publications. Immunogen corresponding to Synthetic Peptide within Human EZR phospho T567.

View Alternative Names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (AB47293)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (phospho T567) antibody (AB47293)

ab47293 staining human normal placenta. Staining is localised to apical membrane and cytoplasmic compartiment.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may b

Western blot - Anti-Ezrin (phospho T567) antibody (AB47293)
  • WB

Unknown

Western blot - Anti-Ezrin (phospho T567) antibody (AB47293)

The lower band is likely to be Moesin, which has a homologous immunogenic region.

All lanes:

Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)

Lane 1:

Jurkat cell lysate with PMA (125 ng/ml, 30 minutes)

Lane 2:

Jurkat cell lysate

Predicted band size: 69 kDa

Observed band size: 68 kDa,69 kDa

false

Western blot - Anti-Ezrin (phospho T567) antibody (AB47293)
  • WB

Unknown

Western blot - Anti-Ezrin (phospho T567) antibody (AB47293)

All lanes:

Western blot - Anti-Ezrin (phospho T567) antibody (ab47293)

Lane 1:

EGF treated A431 cells, no added peptide

Lane 2:

Untreated A431 cells, no added peptide

Predicted band size: 69 kDa

Observed band size: 69 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human EZR phospho T567. The exact immunogen used to generate this antibody is proprietary information.

P15311

Specificity

This antibody detects endogenous levels of Ezrin only when phosphorylated at Threonine 567. This antibody will also react with Moesin at Threonine 577 and Radixin at Threonine 564.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was purified using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
Biological function summary

Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.

Pathways

Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.

Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
See full target information EZR phospho T567
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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 43:114941 PubMed39636728

2024

Myosin 1b regulates intestinal epithelial morphogenesis via interaction with UNC45A.

Applications

Unspecified application

Species

Unspecified reactive species

Céline Revenu,Corinne Lebreton,Magda Cannata Serio,Marion Rosello,Rémi Duclaux-Loras,Karine Duroure,Ophélie Nicolle,Fanny Eggeler,Marie-Thérèse Prospéri,Julie Stoufflet,Juliette Vougny,Priscilla Lépine,Grégoire Michaux,Nadine Cerf-Bensussan,Evelyne Coudrier,Franck Perez,Marianna Parlato,Filippo Del Bene

Journal of cellular and molecular medicine 28:e18375 PubMed39039796

2024

Celastrol alleviates atopic dermatitis by regulating Ezrin-mediated mitochondrial fission and fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Wang,Shan Jin,Hanye Liu,Xinyi Song,Hongyu Jin,Yilan Song,Hongwei Zhao,Liangchang Li,Guanghai Yan

Journal of Cancer 15:2448-2459 PubMed38577590

2024

Nudifloside, a Secoiridoid Glucoside Derived from Callicarpa nudiflora, Inhibits Endothelial-to-Mesenchymal Transition and Angiogenesis in Endothelial Cells by Suppressing Ezrin Phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Dongliang Zhuo,Yinlong Mei,Chaozhan Lin,Aizhi Wu,Yuehua Luo,Hong Lu,Jianjiang Fu

Frontiers in physiology 14:1108304 PubMed36926194

2023

Hypoxia enhances interactions between Na/H exchanger isoform 1 and actin filaments ezrin in pulmonary vascular smooth muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Julie M Lade,Manuella R Andrade,Clark Undem,Jasmine Walker,Haiyang Jiang,Xin Yun,Larissa A Shimoda

International journal of biological sciences 18:1238-1253 PubMed35173550

2022

Poly (ADP-ribose) polymerase 1 (PARP1) inhibition promotes pulmonary metastasis of osteosarcoma by boosting ezrin phosphorylation.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfei Li,Xiaoqiu Wu,Xuekun Fu,Jin Liu,Wangze Song,Gary Guishan Xiao,Aiping Lu,Ge Zhang

Molecules (Basel, Switzerland) 26: PubMed34577118

2021

Ezrin Modulates the Cell Surface Expression of Programmed Cell Death Ligand-1 in Human Cervical Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chihiro Tanaka,Takuro Kobori,Mayuka Tameishi,Yoko Urashima,Takuya Ito,Tokio Obata

Scientific reports 10:8189 PubMed32424125

2020

Nanostructural Diversity of Synapses in the Mammalian Spinal Cord.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Broadhead,Calum Bonthron,Lauren Arcinas,Sumi Bez,Fei Zhu,Frances Goff,Jonathan Nylk,Kishan Dholakia,Frank Gunn-Moore,Seth G N Grant,Gareth B Miles

Journal of Cancer 11:1584-1595 PubMed32047564

2020

Effects of Galectin-1 on Biological Behavior in Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Mandika Chetry,Yizuo Song,Chunyu Pan,Ruyi Li,Jianan Zhang,Xueqiong Zhu

Medical science monitor : international medical jo 24:2098-2108 PubMed29628496

2018

Ezrin/NF-κB Pathway Regulates EGF-induced Epithelial-Mesenchymal Transition (EMT), Metastasis, and Progression of Osteosarcoma.

Applications

WB

Species

Unspecified reactive species

Peng Liu,Peng Yang,Zhang Zhang,Mingfa Liu,Sanbao Hu

The Journal of biological chemistry 293:8242-8254 PubMed29599290

2018

TGFβ and IGF1R signaling activates protein kinase A through differential regulation of ezrin phosphorylation in colon cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Premila D Leiphrakpam,Michael G Brattain,Jennifer D Black,Jing Wang
View all publications
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