Rabbit Monoclonal Ezrin phospho Y353 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (AB284696), expandable thumbnail](https://content.abcam.com/products/images/ezrin-phospho-y353-antibody-ep925y-bsa-and-azide-free-ab284696--western-blot-img175338.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (AB284696)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.
Alternative names
Ezrin, Cytovillin, Villin-2, p81, VIL2, EZR
Recommended products
Rabbit Monoclonal Ezrin phospho Y353 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Alternative names
Ezrin, Cytovillin, Villin-2, p81, VIL2, EZR
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP925Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab284696 is the carrier-free version of Anti-Ezrin (phospho Y353) antibody [EP925Y] ab52916
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Ezrin also known as cytovillin or villin-2 is a protein belonging to the ezrin-radixin-moesin (ERM) family. It is approximately 81 kDa in molecular weight. Ezrin plays a critical role in linking the actin cytoskeleton to the plasma membrane. This interaction occurs through its ability to bind to membrane proteins and phospholipids facilitating structural support and signal transduction. Ezrin is widely expressed in epithelial tissues but it also occurs in other cell types such as lymphocytes and osteoclasts.
- Biological function summary
Ezrin functions as a membrane-microfilament linker and is essential for maintaining cell shape adhesion and motility. It is an integral component of many cellular structures including microvilli and ruffles. Ezrin’s activity often involves forming complexes with other proteins such as EBP50 and F-actin which are fundamental for its role in cellular morphogenesis. These interactions allow ezrin to regulate membrane surface structure and cell surface organization.
- Pathways
Ezrin participates in various signaling pathways that are essential for cell migration and invasion. One key pathway is the Rho family of GTPases which regulates actin cytoskeleton dynamics. Ezrin interacts with proteins like Rho-associated kinase (ROCK) that influence cell contraction and motility. Additionally ezrin is involved in the MAPK/ERK pathway which influences cell growth and survival by interacting with other signaling molecules.
- Associated diseases and disorders
Ezrin shows significant relevance to cancer progression and metastasis particularly in conditions like osteosarcoma. Ezrin overexpression correlates with increased metastatic potential and poor clinical outcomes. It also has implications in allergic diseases due to its role in immune cell function. In these contexts ezrin interacts with SRC family kinases which are important for transmitting signals from cell-surface receptors to downstream pathways influencing disease development and progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
![Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (ab284696), expandable thumbnail](https://content.abcam.com/products/images/ezrin-phospho-y353-antibody-ep925y-bsa-and-azide-free-ab284696--western-blot-img175338.jpg "Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (ab284696)")
Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (ab284696)
This data was developed using Anti-Ezrin (phospho Y353) antibody [EP925Y] ab52916, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Ezrin (phospho Y353) antibody [EP925Y] - BSA and Azide free (ab284696) at 1/200000 dilution
Lane 1: 293 cell lysate untreated at 10 µg
Lane 2: 293 cell lysate treated with pervanadate at 10 µg
Predicted band size: 69 kDa
Observed band size: 69 kDa
Downloads
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