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Rabbit Recombinant Monoclonal Ezrin phospho T567 antibody. Suitable for IHC-P, Dot, WB and reacts with Mouse, Rat, Human, Synthetic peptide samples. Cited in 11 publications.


Images

Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247), expandable thumbnail
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247), expandable thumbnail
  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (AB76247), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytIHC-PDotWB
Human
Not recommended
Tested
Expected
Tested
Mouse
Not recommended
Tested
Expected
Tested
Rat
Not recommended
Tested
Expected
Tested
Synthetic peptide
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human, Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/100 - 1/250
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Mouse, Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide
Dilution info
-
Notes

-

Target data

Function

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Additional Targets

Moesin phospho T558, Radixin phospho T564

Alternative names

VIL2, EZR, Ezrin, Cytovillin, Villin-2, p81

Recommended products

Rabbit Recombinant Monoclonal Ezrin phospho T567 antibody. Suitable for IHC-P, Dot, WB and reacts with Mouse, Rat, Human, Synthetic peptide samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP2122Y
Purification technique
Affinity purification Protein A
Specificity

ab76247 will detect Ezrin, Radixin and Moesin.
This antibody detects the phosphorylated target and not the non-phosphorylated epitope.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Ezrin radixin and moesin are referred to collectively as the ERM proteins. These proteins mechanically link the actin cytoskeleton to the plasma membrane helping to maintain cell shape and surface structure. Ezrin is often identified with a mass of approximately 82 kDa. ERM proteins are expressed in various tissues such as epithelial cells where they influence cellular morphology and dynamics.

Biological function summary

ERM proteins play a role in signal transduction maintaining cellular architecture and modulating membrane dynamics. As a part of the ERM complex they regulate cytoskeletal rearrangements which are necessary during cellular processes like migration and adhesion. These functions are critical for maintaining cell stability and interaction with the extracellular environment.

Pathways

ERM proteins influence a number of signaling cascades including the PI3K/Akt pathway and the Rho GTPase signaling pathway. These pathways are integral to cell survival proliferation and movement. Through these pathways ERM proteins interact with related proteins such as RhoA and PTEN thereby influencing cellular responses to external stimuli.

Associated diseases and disorders

ERM proteins have connections to cancer metastasis and neurodegenerative diseases. In cancer altered ERM expression levels can affect tumor progression and metastasis with interactions involving proteins like CD44. In neurodegenerative conditions changes in the regulation of ERM proteins might impact cell survival and tissue integrity. Understanding these relationships helps in developing targeted therapies for managing these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) Western blot staining using rabbit Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1: Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Lane 2: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Lane 4: Untreated HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 15 µg

    Lane 5: HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for 60 minutes whole cell lysate at 15 µg

    Lane 6: HEK-293 (Human embryonic kidney epithelial cell) starved overnight, then treated with 100nM Calyculin A for g0 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa

    Observed band size: 75-81 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) Western blot staining using rabbit Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1: Untreated NIH/3T3 (Mouse embyonic fibroblast) whole cell lysate at 15 µg

    Lane 2: Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate at 15 µg

    Lane 3: Untreated NIH/3T3 (Mouse embyonic fibroblast) treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa

    Observed band size: 75-81 kDa

    Exposure time: 180s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) Western blot staining using rabbit Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247) at 1/1000 dilution

    Lane 1: Untreated C6 (Rat glial tumor glial cell) whole cell lysate at 15 µg

    Lane 2: Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate at 15 µg

    Lane 3: Untreated C6 (Rat glial tumor glial cell) treated with 100ng/ml Calyculin A for 60 minutes whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 69 kDa

    Observed band size: 75-81 kDa

    Exposure time: 5s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) with ab76247 at 1/500 (1.64 μg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Membranous staining on human breast.

    The section was incubated with ab76247 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Counterstained with Hematoxylin.

  • Dot Blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247), expandable thumbnail

    Dot Blot - Anti-Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) antibody [EP2122Y] (ab76247)

    Dot blot analysis of Ezrin (pThr567)/ Radixin (pThr564)/ Moesin (pThr558) phospho peptide (Lane 1), Non-phospho peptide (Lane 2), labelling Ezrin (phospho Thr567)/ Radixin (phospho Thr564)/ Moesin (phospho Thr558) with ab76247 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The sequence of non-phospho peptide: CGRDKYKTLRQIR.
    The sequence of phospho peptide: CGRDKYK-pT-LRQIR.

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Product protocols

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