Anti-F-actin antibody [4E3.adl] (ab130935) is a mouse monoclonal antibody that is used to detect F-actin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human, Mouse samples.
- Over 20 publications
Images
![Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--western-blot-img25896.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935)")
![Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--flow-cytometry-intracellular-img38630.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935)")
![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--immunocytochemistry-immunofluorescence-img134979.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935)")
Publications
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- Iranian journal of basic medical sciences 26:1390-13992023Short-hairpin RNA-mediated suppression of cortactin may inhibit the migration and invasion abilities of endometrial cancer cells by reducing lamellipodia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuisi Lin,Yujuan Fan,Zhifu Zhi,Lihong Pang,Dan SunPubMed 37970440
- Discover. Oncology 14:1912023ZDHHC9: a promising therapeutic target for triple-negative breast cancer through immune modulation and immune checkpoint blockade resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Xu,Yongjie Xie,Peng Xie,Jianming Li,Zhongsheng Tong,Yanfang YangPubMed 37875591
- Molecular & cellular oncology 10:22388732023Silencing Glypican-1 enhances the antitumor effects of Pictilisib via downregulating PI3K/Akt/ERK signaling in chemo-resistant esophageal adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkshay Pratap,Andrea Qualman,Hedlund Garrett,Lindsey Westbrook,Erlinda The,Sanchayita Mitra,Mila Cordero,Kenneth Meza Monge,Juan-Pablo Idrovo,Argudit Chauhan,Linling Cheng,Mitchell Jay Cohen,Benedetto Mungo,Sachin Wani,Robert Alexander Meguid,Martin D McCarter,Xianzhong MengPubMed 37649964
- Aging 15:5514-55342023hucMSCs treatment prevents pulmonary fibrosis by reducing circANKRD42-YAP1-mediated mechanical stiffness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaitong Zhang,Qi Zhu,Yunxia Ji,Meirong Wang,Qian Zhang,Weili Liu,Ruiqiong Li,Jinjin Zhang,Pan Xu,Xiaodong Song,Changjun LvPubMed 37335082
- Journal of anatomy 243:684-6892023Structure of rosettes in the zona glomerulosa of human adrenal cortex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYumin Zhu,Xuefeng Zhang,Changlong HuPubMed 37294692
- Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie 261:1321-13292022The usefulness of lutein/trypan blue vital dye for the staining of corneal endothelium: a pilot study on DMEK pretreated tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRossella Colabelli Gisoldi,Gemma Lodato,Bijorn Omar Balzamino,Graziana Esposito,Alessandra Micera,Augusto PocobelliPubMed 36445446
- Cells 11:2022Endothelial Barrier Disruption by Lipid Emulsions Containing a High Amount of N3 Fatty Acids (Omegaven) but Not N6 Fatty Acids (Intralipid).Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmilie Gueguen,Yasser Morsy,Michael Scharl,Stefanie D Krämer,Michael Zaugg,Martin Hersberger,Gerhard Rogler,Marcin WawrzyniakPubMed 35883643
- Frontiers in medicine 9:8613712022Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong ZhuoPubMed 35492354
- Journal of experimental & clinical cancer research : CR 41:892022Subtypes in pancreatic ductal adenocarcinoma based on niche factor dependency show distinct drug treatment responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomohiko Shinkawa,Kenoki Ohuchida,Yuki Mochida,Kukiko Sakihama,Chika Iwamoto,Toshiya Abe,Noboru Ideno,Yusuke Mizuuchi,Koji Shindo,Naoki Ikenaga,Taiki Moriyama,Kohei Nakata,Yoshinao Oda,Masafumi NakamuraPubMed 35272688
- Molecules (Basel, Switzerland) 27:2022Betulonic Acid, as One of the Active Components of the Extract, Inhibits the Invasion and Metastasis of Gastric Cancer Cells by Mediating Cytoskeleton Rearrangement In Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZewen Chu,Yuanyuan Luo,Tengyang Ni,Miao Zhu,Xinyi Feng,Yanqing Liu,Haibo WangPubMed 35164287
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt (Intra) | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Expected | Tested | Expected |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.10000-1.00000 µg for 106 Cells | Notes Mouse IgM [B11/7] - Isotype control ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Abcam recommends blocking with 3% Milk |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
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Target data
Function
Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).
Alternative names
Beta-actin, ACTB
Recommended products
Anti-F-actin antibody [4E3.adl] (ab130935) is a mouse monoclonal antibody that is used to detect F-actin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, ICC/IF. Suitable for Human, Mouse samples.
- Over 20 publications
Key facts
- Isotype
- IgM
- Host species
- Mouse
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 4E3.adl
- Purification technique
- Cross-flow filtration
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-F-actin antibody [4E3.adl] (ab130935) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of F-actin?
Anti-F-actin [4E3.adl] (ab130935) specifically detects a band for F-actin (UniProt: P68139) at a molecular weight of 42kDa.
Trusted by the scientific community
Anti-F-actin [4E3.adl] (ab130935) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.
- Biological function summary
The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.
- Pathways
F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.
- Associated diseases and disorders
Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--western-blot-img25896.jpg "Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)")
Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)
F-actin Western blot staining using mouse Anti-F-actin antibody
All lanes: Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935) at 1/500 dilution
Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg
Lane 2: Skeletal Muscle (Mouse) Tissue Lysate at 20 µg
Lane 3: Heart (Mouse) Tissue Lysate at 20 µg
Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Lane 5: C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 20 µg
SecondaryAll lanes: Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM (ab98112) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 42 kDa, 60 kDa, 70 kDa
Exposure time: 10s
![Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--flow-cytometry-intracellular-img38630.jpg "Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)")
Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)
F-actin Flow Cytometry (Intracellular) staining using mouse Anti-F-actin antibody
Overlay histogram showing HeLa cells stained with ab130935 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130935, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat Anti-mouse DyLight® 488 (IgM; mu chain, Goat Anti-Mouse IgM mu chain (DyLight® 488) ab97007) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (Mouse IgM [B11/7] - Isotype control ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.
![Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935), expandable thumbnail](https://content.abcam.com/products/images/f-actin-antibody-4e3adl-loading-control-ab130935--immunocytochemistry-immunofluorescence-img134979.jpg "Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)")
Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)
F-actin Immunocytochemistry/ Immunofluorescence staining using mouse Anti-F-actin antibody
ab130935 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab130935 at 5μg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-mouse DyLight® 488 (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
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