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Mouse Monoclonal beta Actin antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse samples. Cited in 28 publications.

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Images

Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] (AB130935), expandable thumbnail
  • Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (AB130935), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] (AB130935), expandable thumbnail

Publications

Key facts

Isotype
IgM
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS

Form
Liquid
Clonality
Monoclonal

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBICC/IF
Human
Tested
Expected
Tested
Mouse
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
0.10000-1.00000 µg for 106 Cells
Notes

Mouse IgM [B11/7] - Isotype control ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Abcam recommends blocking with 3% Milk

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
5 µg/mL
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed:25255767, PubMed:29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed:29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed:29925947). Part of the ACTR1A/ACTB filament around which the dynactin complex is built. The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

Alternative names

Beta-actin, ACTB

Recommended products

Mouse Monoclonal beta Actin antibody. Suitable for Flow Cyt (Intra), WB, ICC/IF and reacts with Human, Mouse samples. Cited in 28 publications.

Key facts

Isotype
IgM
Form
Liquid
Clonality
Monoclonal
Clone number
4E3.adl
Purification technique
Cross-flow filtration
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.

Biological function summary

The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.

Pathways

F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.

Associated diseases and disorders

Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] (ab130935), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-F-actin antibody [4E3.adl] (ab130935)

    Overlay histogram showing HeLa cells stained with ab130935 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130935, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat Anti-mouse DyLight® 488 (IgM; mu chain, Goat Anti-Mouse IgM mu chain (DyLight® 488) ab97007) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (Mouse IgM [B11/7] - Isotype control ab91545, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

  • Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935), expandable thumbnail

    Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935)

    F-actin Western blot staining using mouse Anti-F-actin antibody

    All lanes: Western blot - Anti-F-actin antibody [4E3.adl] - Loading Control (ab130935) at 1/500 dilution

    Lane 1: Human skeletal muscle tissue lysate - total protein (ab29330) at 20 µg

    Lane 2: Skeletal Muscle (Mouse) Tissue Lysate at 20 µg

    Lane 3: Heart (Mouse) Tissue Lysate at 20 µg

    Lane 4: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

    Lane 5: C2C12 (Mouse myoblast cell line) Whole Cell Lysate at 20 µg

    Secondary

    All lanes: Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM (ab98112) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 41 kDa

    Observed band size: 42 kDa, 60 kDa, 70 kDa

    Exposure time: 10s

  • Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] (ab130935), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [4E3.adl] (ab130935)

    ab130935 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab130935 at 5μg/ml overnight at +4°C. The secondary antibody (green) was a goat anti-mouse DyLight® 488 (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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