AB205

Anti-F-actin antibody [NH3]

Name: Anti-F-actin antibody [NH3] - mouse monoclonal (ab205) | Abcam
Brand: Abcam Limited
SKU: AB205
Price: 450 USD
Availability: InStock
Rating: 4 (11 reviews)

(11 Reviews)

(167 Publications)

Anti-F-actin antibody [NH3] (ab205) is a mouse monoclonal antibody detecting F-actin in Western Blot, Flow Cytometry, IHC-Fr, ICC/IF, ELISA. Suitable for Human, Mouse, Rabbit, Rat.

- Over 120 publications
- Trusted since 1999

View Alternative Names

Beta-actin, ACTB

3 Images

Supplier Data

Western blot - Anti-F-actin antibody [NH3] (AB205)

All lanes:

Western blot - Anti-F-actin antibody [NH3] (ab205)

All lanes:

HeLa whole cell lysate

Secondary

All lanes:

HRP conjugated Rabbit F(ab')2 anti Mouse IgG (Human Adsorbed) at 1/2000 dilution

Predicted band size: 40 kDa

true

Exposure time: 22s

Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (AB205)

ICC/IF

CiteAb

Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (AB205)

F-actin immunocytochemistry-immunofluorescence using Anti-F-actin antibody [NH3] ab205. Publication image and figure legend from Luo, W. D., Min, J. W., et al., 2018, Journal of Neuroinflammation PubMed 29925377.

Vitexin downregulated F-actin expression in RBMECs after OGD. FITC-conjugated phalloidin staining for F-actin+ stress fiber formation demonstrated changes in cytoskeletal assembly following OGD in RBMECs. a Confocal images show co-localization of NKCC1 and F-actin in RBMECs. Scale bar = 10 μm. b Merged views of indicated NKCC1 show complete co-localization (b, left). A side overlap of two peaks was taken as a partial co-localization (b, right). c Pearson's correlation coefficient is shown in graph (c) from five independent experiments analyzed. d, e The distribution of F-actin+ stress fibers was shown by confocal imaging in Control, OGD, OGD+Bum, and OGD+Vit groups. Arrows indicate actin stress fiber formation. The intensity of F-actin fluorescence was determined in 10 fields/well and divided by the number of cells counterstained with DAPI. Each experimental group consisted of three replicates. Scale bar = 10 μm

CiteAb

Western blot - Anti-F-actin antibody [NH3] (AB205)

F-actin western blot using Anti-F-actin antibody [NH3] ab205. Publication image and figure legend from Ji, K., Lin, K., et al., 2018, Cancer Cell International, PubMed 30127666.

TAZ modulates mitochondrial fission via the JNK/F-actin pathway. a-c Western blotting was used to analyze JNK activity and F-actin expression. IL-2 treatment promoted JNK phosphorylation and F-actin upregulation; this effect was further enhanced by TAZ deletion. To inhibit the activity of JNK, SP600125 (SP) was added to TAZ-depleted cells. To activate the JNK pathway, anisomycin (Ani) was added to control cells. d, e Immunofluorescence staining of phosphorylated JNK following TAZ deletion and IL-2 treatment. f, g Immunofluorescence staining for F-actin following JNK activation or inhibition. h, i Immunofluorescence staining of mitochondria. The average number of mitochondria was recorded. *p < < 0.05 vs. control (Ctrl) group; #p < < 0.05 vs. IL-2 group; @p < < 0.05 vs. IL-2 + si-TAZ group

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NH3

Isotype

IgM

Light chain type

unknown

Carrier free

Reacts with

Rat, Rabbit, Human, Mouse

Applications

WB, IHC-R, ELISA, IHC-Fr, ICC/IF, Flow Cyt

applications

Epitope

This antibody is reported to recognise actin in the filamentous form with the epitope likely to be located between residues 120 and 226 of the molecule.

Specificity

Clone NH3 recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCR" : {"fullname" : "Immunohistochemistry (Resin sections)", "shortname":"IHC-R"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.", "IHCR-species-checked": "guaranteed", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.", "IHCR-species-checked": "guaranteed", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.", "IHCR-species-checked": "guaranteed", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rabbit": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igm-b11-7-isotype-control-ab91545'>ab91545</a> - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody must be used to detect this primary.", "IHCR-species-checked": "guaranteed", "IHCR-species-dilution-info": "", "IHCR-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

style

left-column

Product details

Product Specifications

Anti-F-actin antibody [NH3] (ab205) is a mouse monoclonal antibody and is validated for use in ELISA, Flow Cyt, ICC/IF, IHC-Fr, IHC-R, IP, WB in human, mouse, rabbit, rat samples.
Anti-F-actin antibody [NH3] (ab205) specifically detects F-actin (UniProt ID: P60709; Molecular weight: 42kDa) and is sold in 250 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-F-actin antibody [NH3] (ab205) has high sensitivity and specificity.
Anti-F-actin antibody [NH3] (ab205) has been cited over 126 times in peer reviewed journals and is trusted by the scientific community.
Anti-F-actin antibody [NH3] (ab205) has 11 independent reviews from customers.

style

left-column

Properties and storage information

Form

Liquid

Purity

IgM fraction

Purification notes

Purified IgM prepared from tissue culture supernatant.

Storage buffer

Preservative: 0.1% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F-actin also known as filamentous actin is an essential structural protein found within the cytoskeleton of eukaryotic cells. Its alternate names include actin filaments or microfilaments. The protein consists of polymerized monomers of G-actin each with a molecular weight of roughly 42 kDa. F-actin is expressed abundantly in muscle cells and non-muscle cells alike providing structural support and facilitating cellular movements. Actin staining is a common method used in labs to visualize these dynamic structures often employing phalloidin staining a toxin that stabilizes actin filaments conjugated with fluorescent labels such as Phalloidin 594 Phalloidin 647 or Phalloidin 488 for imaging purposes.

Biological function summary

The actin cytoskeleton plays integral roles in maintaining cell shape providing mechanical resistance against deformation and driving important cellular processes such as endocytosis cell division and motility. F-actin forms part of numerous protein complexes interacting with other proteins like myosin to facilitate muscle contraction and cellular transport. Within cells F-actin is dynamic readily polymerizing and depolymerizing in response to cellular signaling making it essential for cytoskeletal remodeling and cellular adaptability.

Pathways

F-actin is central to various signaling cascades underlying processes like cell signaling and intracellular transport. Notably it participates in the Rho family GTPase pathway affecting cell cytoskeleton organization and motility. It also interacts with proteins like cofilin and profilin which regulate actin polymerization and treadmilling dynamics respectively. These interactions highlight F-actin's involvement in complex cellular pathway regulation processes essential for maintaining cellular homeostasis and adaptability.

Abnormal regulation or mutations in actin-related proteins can lead to conditions such as cancer and cardiomyopathies. For example during metastasis cancer cells exploit the dynamic nature of F-actin for enhanced migratory capacity. In cardiac muscle cells actin interacts with other proteins like tropomyosin and mutations in these genes can disrupt normal heart function leading to cardiomyopathies. As such F-actin not only represents a critical component of the cellular structure but also serves as a pivotal target for understanding disease mechanisms and potential therapeutic intervention points.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells (PubMed : 25255767, PubMed : 29581253). Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction (PubMed : 29581253). In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA (PubMed : 29925947). Plays a role in the assembly of the gamma-tubulin ring complex (gTuRC), which regulates the minus-end nucleation of alpha-beta tubulin heterodimers that grow into microtubule protafilaments (PubMed : 39321809, PubMed : 38609661). Part of the ACTR1A/ACTB filament around which the dynactin complex is built (By similarity). The dynactin multiprotein complex activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity).

See full target information ACTB

style

left-column

Publications (167)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113353 PubMed40927678

2025

Sustained Mg/Sr ion delivery from injectable silk fibroin hydrogels drives SCAP osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Zhengrong Gao,Yuman Li,Tuanfeng Zhou,Huimin Zheng,Shengjie Jiang,Yue Yang,Yan Wei

Journal of cell communication and signaling 19:e70031 PubMed40620356

2025

Sirtuin 2 regulates NOD-like receptor protein 3/nuclear factor kappa B axis to promote cartilage repair in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotian Chen,Yining Song,Fan Zhang,Fangyan Hu,Zhenfei Ding,Jianzhong Guan

Cell biology international 49:1197-1208 PubMed40616572

2025

COMMD1 Inhibits Epithelial Mesenchymal Transition (EMT) and Liver Metastasis in Cervical Cancer Through Modulation of the Twist1/E-Cadherin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Zhang,Xiuli Wang,Ke Wang,Lei Sun,Shiying Yang,Xiaoguang Wang

Journal of cell communication and signaling 19:e70018 PubMed40584821

2025

Hypoxic adipose-derived stem cell exosomes as carriers of miR-100-5p to enhance angiogenesis and suppress inflammation in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Liu,Fei Hao,Bangtao Chen

American journal of translational research 17:2445-2466 PubMed40385018

2025

Downregulated TRAF3IP2-AS1 promotes hepatocellular carcinoma progression through the miR-374a-5p/SEL1L1/RPL6 axis to enhance DNA damage repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Wang,Wen-Ze Wu,Yuan-Wen Hu,Qian-Qian Yu,Hai-Long Ge,Wei-Xin Yu,Feng Mo,Chen Chao,Dong-Lin Sun

Frontiers in endocrinology 16:1583408 PubMed40343072

2025

Brown adipose tissue-derived extracellular vesicles regulate hepatocyte mitochondrial activity to alleviate high-fat diet-induced jawbone osteoporosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Sha Zhang,Guorong Deng,Guangxiang He,Yuan Yuan,Yu Fu,Yihan Liu,Zhen Gong,Liang Kong,Chenxi Zheng

Experimental & molecular medicine 57:1064-1077 PubMed40335634

2025

Acetylation of α-tubulin restores endothelial cell injury and blood-brain barrier disruption after intracerebral hemorrhage in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejiao Lei,Eryi Sun,Xufang Ru,Yulian Quan,Xuezhu Chen,Qian Zhang,Yougling Lu,Qianying Huang,Yujie Chen,Wenyan Li,Hua Feng,Yang Yang,Rong Hu

Bone research 13:4 PubMed39746903

2025

Neuronal guidance factor Sema3A inhibits neurite ingrowth and prevents chondrocyte hypertrophy in the degeneration of knee cartilage in mice, monkeys and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Shishu Huang,Dashuang Gao,Zhenxia Li,Hongchen He,Xi Yu,Xuanhe You,Diwei Wu,Ze Du,Jiancheng Zeng,Xiaojun Shi,Qinshen Hu,Yong Nie,Zhong Zhang,Zeyu Luo,Duan Wang,Zhihe Zhao,Lingli Li,Guanglin Wang,Liping Wang,Zongke Zhou,Di Chen,Fan Yang

Journal of translational medicine 22:1164 PubMed39741300

2025

RBM47 promotes cell proliferation and immune evasion by upregulating PDIA6: a novel mechanism of pancreatic cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Ma,Enjie Liu,Huijie Fan,Chenfei Li,Pei Huang,Meiying Cui,Zhengyang Wang,Jing Zhou,Kuisheng Chen

Autophagy 21:807-826 PubMed39635882

2024

Upregulation of ISG15 induced by MAPT/tau accumulation represses autophagic flux by inhibiting HDAC6 activity: a vicious cycle in Alzheimer disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Liu,Xin Wang,Zhi-Ting Fang,Jun-Ning Zhao,Xue-Xiang Rui,Bing-Ge Zhang,Ye He,Rui-Juan Liu,Jian Chen,Gao-Shang Chai,Gong-Ping Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-F-actin antibody [NH3]

Western blot - Anti-F-actin antibody [NH3] (AB205)

Immunocytochemistry/ Immunofluorescence - Anti-F-actin antibody [NH3] (AB205)

Western blot - Anti-F-actin antibody [NH3] (AB205)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Epitope

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Reagents, Controls & Accessories

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (167)

Product promise