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AB215165

Anti-F-spondin antibody

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(1 Publication)

Rabbit Polyclonal F-spondin antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SPON1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0762, VSGP, SPON1, Spondin-1, F-spondin, Vascular smooth muscle cell growth-promoting factor

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling F-spondin with ab215165 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling F-spondin with ab215165 at 1/400 dilution and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F-spondin antibody (AB215165)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling F-spondin with ab215165 at 1/400 dilution and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human SPON1 aa 750 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9HCB6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F-spondin also known as spondin-1 acts as an extracellular matrix protein that influences cell adhesion and guidance. It has a mass of approximately 100 kDa and consists of thrombospondin-type 1 repeats (TSR) and a C-terminal region with homology to the F-spondin family. This protein is predominantly expressed in the nervous system particularly in the floor plate of the developing vertebrate embryos. It is also found in the adult central nervous system.
Biological function summary

F-spondin promotes neuronal growth and synaptic function by influencing axon guidance and cellular adhesion properties. It interacts with cell surface receptors and extracellular matrix components to facilitate these processes. F-spondin is not part of a complex by itself but it mediates important interactions between different molecular entities necessary for proper cellular function.

Pathways

F-spondin plays significant roles in both the Wnt signaling pathway and the Notch signaling pathway. These pathways are essential in regulating cellular growth and differentiation. F-spondin interacts with various proteins such as integrins and neuronal receptors which modulate these processes. The connection between F-spondin and these pathways highlights its influence on tissue development and cellular communication.

F-spondin has shown relevance to Alzheimer's disease and spinal cord injury. In Alzheimer's disease disrupted F-spondin interactions may lead to impaired neuronal connectivity. It also associates with amyloid precursor protein (APP) through this neurological disorder. In spinal cord injuries F-spondin's ability to promote axonal growth suggests potential roles in recovery and repair. Its interaction with extracellular matrix proteins highlights its therapeutic potential in enhancing neural regeneration.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell adhesion protein that promotes the attachment of spinal cord and sensory neuron cells and the outgrowth of neurites in vitro. May contribute to the growth and guidance of axons in both the spinal cord and the PNS (By similarity). Major factor for vascular smooth muscle cell.
See full target information SPON1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 133: PubMed36647821

2023

Karyopherin α deficiency contributes to human preimplantation embryo arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Wang,Yoichi Miyamoto,Biaobang Chen,Juanzi Shi,Feiyang Diao,Wei Zheng,Qun Li,Lan Yu,Lin Li,Yao Xu,Ling Wu,Xiaoyan Mao,Jing Fu,Bin Li,Zheng Yan,Rong Shi,Xia Xue,Jian Mu,Zhihua Zhang,Tianyu Wu,Lin Zhao,Weijie Wang,Zhou Zhou,Jie Dong,Qiaoli Li,Li Jin,Lin He,Xiaoxi Sun,Ge Lin,Yanping Kuang,Lei Wang,Qing Sang
View all publications
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