AB314103

Anti-F4/80 antibody [BLR209K] - BSA free

Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal F4/80 antibody. Suitable for ICC, IHC-P, IP, WB and reacts with Mouse samples. Immunogen corresponding to Recombinant Fragment Protein within Mouse Adgre1 aa 1-250.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Detection of mouse F4/80 by immunohistochemistry. Sample : FFPE section of mouse spleen. Antibody : Rabbit anti-F4/80 recombinant monoclonal antibody [BLR209K] (A700-209). Secondary : HRP-conjugated goat antirabbit IgG.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Detection of mouse F4/80 by immunohistochemistry. Sample : FFPE section of mouse gut. Antibody : Rabbit antiF4/80 recombinant monoclonal antibody [BLR209K] (A700-209). Secondary : HRP-conjugated goat antirabbit IgG.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Detection of mouse F4/80 by immunohistochemistry. Sample : FFPE section of mouse liver. Antibody : Rabbit antiF4/80 recombinant monoclonal antibody [BLR209K] (A700-209). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunocytochemistry - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Detection of mouse F4/80 by immunocytochemistry. Sample : FFPE section of J774A1 cells. Antibody : Rabbit anti-F4/80 recombinant monoclonal antibody [BLR209K] (A700-209). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Detection of mouse F4/80 by immunocytochemistry. Sample : FFPE section of RAW 264.7 cells. Antibody : Rabbit anti-F4/80 recombinant monoclonal antibody [BLR209K] (A700-209). Secondary : HRP-conjugated goat antirabbit IgG.

Supplier Data

Immunoprecipitation - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Samples : Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from RAW 264.7 cells prepared using NETN lysis buffer. Antibodies : Rabbit antiF4/80 recombinant monoclonal antibody [BLR209K] (A700-209) used for IP at 6 µl/mg lysate. F4/80 was also immunoprecipitated by a second antibody against a different epitope of F4/80 (BL-2189A-1B8). For blotting immunoprecipitated F4/80, A700-209 was used at 1 : 1000. Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-F4/80 antibody [BLR209K] - BSA free (ab314103) at 1/1000 dilution

All lanes:

RAW 264.7 cells

false

Supplier Data

Western blot - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Samples : Whole cell lysate (5 µg) from RAW 264.7, NIH 3T3, Ba/F3, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-F4/80 recombinant monoclonal antibody [BLR209K] (A700-209) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel : Rabbit anti-COPB2 antibody.

All lanes:

Western blot - Anti-F4/80 antibody [BLR209K] - BSA free (ab314103) at 1/1000 dilution

Lane 1:

RAW 264.7 Whole cell lysate at 5 µg

Lane 2:

NIH 3T3 Whole cell lysate at 5 µg

Lane 3:

Ba/F3 Whole cell lysate at 5 µg

Lane 4:

CH27 Whole cell lysate at 5 µg

Lane 5:

TCMK-1 Whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR209K

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IP, ICC, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Mouse Adgre1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q61549

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style

left-column

Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-F4/80 antibody [BLR209K] - BSA free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Immunocytochemistry - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Immunocytochemistry - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Immunoprecipitation - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Western blot - Anti-F4/80 antibody [BLR209K] - BSA free (AB314103)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise