Chicken Polyclonal F4/80 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Adgre1.
IgY
Chicken
Preservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose
Liquid
Polyclonal
WB | ICC/IF | |
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Human | Predicted | Predicted |
Mouse | Tested | Tested |
Rat | Predicted | Predicted |
Species | Dilution info | Notes |
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Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
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Species Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Human | Dilution info - | Notes - |
Select an associated product type
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor
Chicken Polyclonal F4/80 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Adgre1.
IgY
Chicken
Preservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose
Liquid
Polyclonal
IgY fraction
Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.
F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.
F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.
F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/mL
All lanes: Mouse spleen lysate
Developed using the ECL technique.
Predicted band size: 97 kDa
Exposure time: 5s
Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.
Image collected and cropped by CiteAb under a CC-BY license from the publication
F4/80 western blot using anti-F4/80 antibody - C-terminal ab186073. Publication image and figure legend from Amini-Nik, S., Sadri, A. R., et al., 2018, Exp Mol Med, PubMed 30026607.
ab186073 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186073 please see the product overview.
Myeloid cells have an essential role in the induction of fibrosis post-thermal injury. a Mice treated with liposomal clodronate compared with control treated group. Trichrome staining shows less fibrosis around portal venules post-thermal injury when myeloid cells are depleted; n = 9, scale bar 50 μm. b Immunohistochemistry shows a highly effective ablation of F4/80+ cells in liposomal clodronate-treated group in comparison to the control group; n = 9, scale bar 50 μm. c There is a global ablation of F4/80+ cells in the liver of mice treated with liposomal clodronate, scale bar 100 μm. d In contrast to earlier findings, without F4/80+ cells, there is an increase in desmin+ cells post-thermal injury; n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
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