Skip to main content

Chicken Polyclonal F4/80 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Adgre1.


Images

Western blot - Anti-F4/80 antibody - C-terminal (AB186073), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (AB186073), expandable thumbnail
  • Western blot - Anti-F4/80 antibody - C-terminal (AB186073), expandable thumbnail

Publications

Key facts

Isotype

IgY

Host species

Chicken

Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 98% PBS, 1% Trehalose

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Mouse Adgre1. The exact immunogen used to generate this antibody is proprietary information. Database link Q61549

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Predicted
Predicted
Mouse
Tested
Tested
Rat
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

0.50000-1.00000 µg/mL

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

-

Notes

-

Predicted
Predicted

Species

Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

8 products for Alternative Product

Target data

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Alternative names

Recommended products

Chicken Polyclonal F4/80 antibody. C-terminal. Suitable for WB, ICC/IF and reacts with Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Mouse Adgre1.

Key facts

Isotype

IgY

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Mouse Adgre1. The exact immunogen used to generate this antibody is proprietary information. Database link Q61549
Purity

IgY fraction

Concentration
Loading...
Purification notes

Crude antiserum was purified by Thiopropyl Sepharose affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

Associated diseases and disorders

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-F4/80 antibody - C-terminal (ab186073), expandable thumbnail

    Western blot - Anti-F4/80 antibody - C-terminal (ab186073)

    All lanes: Western blot - Anti-F4/80 antibody - C-terminal (ab186073) at 1 µg/mL

    All lanes: Mouse spleen lysate

    Developed using the ECL technique.

    Predicted band size: 97 kDa

    Exposure time: 5s

  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody - C-terminal (ab186073)

    Chicken anti mouse F4/80 (ab186073, polyclonal) staining of mouse RAW cells by immunofluorescence, using a FITC-conjugated anti chicken antibody as a secondary antibody.

  • Western blot - Anti-F4/80 antibody - C-terminal (ab186073), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-F4/80 antibody - C-terminal (ab186073)

    F4/80 western blot using anti-F4/80 antibody - C-terminal ab186073. Publication image and figure legend from Amini-Nik, S., Sadri, A. R., et al., 2018, Exp Mol Med, PubMed 30026607.

    ab186073 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186073 please see the product overview.

    Myeloid cells have an essential role in the induction of fibrosis post-thermal injury. a Mice treated with liposomal clodronate compared with control treated group. Trichrome staining shows less fibrosis around portal venules post-thermal injury when myeloid cells are depleted; n = 9, scale bar 50 μm. b Immunohistochemistry shows a highly effective ablation of F4/80+ cells in liposomal clodronate-treated group in comparison to the control group; n = 9, scale bar 50 μm. c There is a global ablation of F4/80+ cells in the liver of mice treated with liposomal clodronate, scale bar 100 μm. d In contrast to earlier findings, without F4/80+ cells, there is an increase in desmin+ cells post-thermal injury; n = 3. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com