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Anti-F4/80 antibody CI:A3-1 is a rat monoclonal antibody that is used in F4/80 ICC/IF, Flow cytometry. Suitable for Mouse samples.

- ab6640 recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages
- Cited in over 1,090 publications
- Trusted since 2002


Images

Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (AB6640), expandable thumbnail
  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (AB6640), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (AB6640), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (AB6640), expandable thumbnail
  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (AB6640), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Rat
Storage buffer

Preservative: 0.09% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q61549

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IF
Mouse
Tested
Tested

Tested
Tested

Species
Mouse
Dilution info
1/10.00000 - 1/50.00000
Notes

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Mouse
Dilution info
5.00000-10.00000 µg/mL
Notes

We recommend Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody.

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Target data

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Alternative names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

Recommended products

Anti-F4/80 antibody CI:A3-1 is a rat monoclonal antibody that is used in F4/80 ICC/IF, Flow cytometry. Suitable for Mouse samples.

- ab6640 recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages
- Cited in over 1,090 publications
- Trusted since 2002

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Cell preparation containing Adgre1 protein. The exact immunogen used to generate this antibody is proprietary information. Database link Q61549
Clone number
CI:A3-1
Purification technique
Affinity purification Protein G
Specificity

Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.

Concentration
Loading...
Purification notes

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) is a rat monoclonal antibody and is validated for use in Flow Cyt (Intra) and ICC/IF.

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) was first used in a scientific publication in 2003 and has been cited over 1093 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) has high sensitivity and specificity.

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) has 68 independent reviews from customers.

Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 96kDa) and is sold in 100 µg selling sizes.

Antibody clone CI:A3-1 is also available pre-conjugated to a variety of labels for your convenience - APC, FITC, PE (ab158, ab15155, ab15156).

F4/80 is a key marker for macrophages, playing a significant role in immune response modulation and inflammation and is linked to various inflammatory diseases. Clone CI:A3 is top cited in the market and a well known immunophenotyping marker in mouse macrophages.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

F4/80 also known as fibroblast-specific protein (FSP1) and by its monoclonal antibody designation BM8 is an established murine macrophage marker. It is a glycoprotein with a molecular weight of approximately 160 kDa. F4/80 is predominantly expressed on the surface of tissue-resident macrophages in mice where it serves as an essential component of the immune system. These macrophages are present across various organs and tissues such as liver (Kupffer cells) lung (alveolar macrophages) spleen and the peritoneal cavity where its expression can be detected using techniques like f4/80 immunofluorescence and f4/80 staining.

Biological function summary

The protein functions in the immune response by promoting phagocytosis and the clearance of apoptotic cells. It contributes to mediating anti-inflammatory responses and is associated with the regulation of cellular immune responses. Although not part of a larger protein complex F4/80 plays a critical role in the differentiation and maturation of macrophages. It is often used as a reliable f4/80 marker in immunohistochemistry (IHC) to identify and study macrophages within tissues.

Pathways

The function of F4/80 influences the Toll-like receptor (TLR) signaling pathway and cytokine production regulatory pathways. These pathways contribute to the innate immune response by recognizing microbial pathogens and activating inflammatory responses. F4/80 interacts with other proteins such as CD11b which is also involved in these immune responses aligning their functions to regulate the activity of macrophages and coordinate with other immune cells.

Associated diseases and disorders

F4/80's involvement in inflammation extends to its role in conditions like atherosclerosis and rheumatoid arthritis. In atherosclerosis the interaction between F4/80 and other proteins like CD36 leads to macrophage accumulation and plaque formation in blood vessels. In rheumatoid arthritis F4/80 expression on specific macrophages contributes to joint inflammation by mediating the secretion of pro-inflammatory cytokines. Both of these conditions highlight the significance of F4/80 in the pathogenesis and progression of diseases characterized by immune dysregulation.

Product promise

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5 product images

  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640), expandable thumbnail

    Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

    F4/80 flow cytometry staining of peritoneal macrophages using rat anti-F4/80 antibody

    Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.

  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640), expandable thumbnail

    Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

    F4/80 flow cytometry staining of J774 cells using rat anti-F4/80 antibody

    Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.

  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

    F4/80 immunofluorescence staining of Raw 264.7 cells using rat anti-F4/80 antibody

    ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5μg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

    F4/80 immunofluorescence staining of mouse bone marrow cells using rat anti-F4/80 antibody

    ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted at 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig diluted 1/200 was used as the secondary antibody.

  • Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640), expandable thumbnail

    Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)

    F4/80 flow cytometry staining of J774 cells using rat anti-F4/80 antibody

    Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.

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Product protocols

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