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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rat anti-F4/80 antibody CI:A3-1 ab6640 is a rat monoclonal antibody that is used in F4/80 immunofluorescence and flow cytometry. Suitable for and mouse samples.
Tried and trusted by researchers since 2002
Anti-F4/80 antibody clone CI:A3-1 is cited in over 1310 publications
Same trusted quality, New lower price!
IgG2b
Rat
Preservative: 0.09% Sodium azide
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | ICC/IF | |
---|---|---|
Mouse | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/10.00000 - 1/50.00000 | Notes ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 5.00000-10.00000 µg/mL | Notes We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Adgre1, Emr1, Gpf480
Rat anti-F4/80 antibody CI:A3-1 ab6640 is a rat monoclonal antibody that is used in F4/80 immunofluorescence and flow cytometry. Suitable for and mouse samples.
Tried and trusted by researchers since 2002
Anti-F4/80 antibody clone CI:A3-1 is cited in over 1310 publications
Same trusted quality, New lower price!
Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor, Adgre1, Emr1, Gpf480
IgG2b
Rat
Preservative: 0.09% Sodium azide
Constituents: PBS
Liquid
Monoclonal
CI:A3-1
Affinity purification Protein G
Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
The protein functions in the immune response by promoting phagocytosis and the clearance of apoptotic cells. It contributes to mediating anti-inflammatory responses and is associated with the regulation of cellular immune responses. Although not part of a larger protein complex F4/80 plays a critical role in the differentiation and maturation of macrophages. It is often used as a reliable f4/80 marker in immunohistochemistry (IHC) to identify and study macrophages within tissues.
F4/80 also known as fibroblast-specific protein (FSP1) and by its monoclonal antibody designation BM8 is an established murine macrophage marker. It is a glycoprotein with a molecular weight of approximately 160 kDa. F4/80 is predominantly expressed on the surface of tissue-resident macrophages in mice where it serves as an essential component of the immune system. These macrophages are present across various organs and tissues such as liver (Kupffer cells) lung (alveolar macrophages) spleen and the peritoneal cavity where its expression can be detected using techniques like f4/80 immunofluorescence and f4/80 staining.
The function of F4/80 influences the Toll-like receptor (TLR) signaling pathway and cytokine production regulatory pathways. These pathways contribute to the innate immune response by recognizing microbial pathogens and activating inflammatory responses. F4/80 interacts with other proteins such as CD11b which is also involved in these immune responses aligning their functions to regulate the activity of macrophages and coordinate with other immune cells.
F4/80's involvement in inflammation extends to its role in conditions like atherosclerosis and rheumatoid arthritis. In atherosclerosis the interaction between F4/80 and other proteins like CD36 leads to macrophage accumulation and plaque formation in blood vessels. In rheumatoid arthritis F4/80 expression on specific macrophages contributes to joint inflammation by mediating the secretion of pro-inflammatory cytokines. Both of these conditions highlight the significance of F4/80 in the pathogenesis and progression of diseases characterized by immune dysregulation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.
Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5μg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43μM for 1hour at room temperature.
ab6640 staining F4/80 in mouse bone marrow cells by immunocytochemistry/ immunofluorescence. Cells were formaldehyde fixed and permeabilized in 0.2% Triton X-100 prior to blocking in 2% BSA for 30 minutes at 20°C. The primary antibody was diluted 1/200 and incubated with the sample for 9 hour at 4°C. Alexa fluor® 488 goat polyclonal to rat Ig, diluted 1/200, was used as the secondary antibody.
Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
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