AB234530

Anti-F4/80 antibody [EPR20548] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal F4/80 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 2 publications.

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Flow Cytometry - Anti-F4/80 antibody [EPR20548] - BSA and Azide free (AB234530)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-F4/80 antibody [EPR20548] - BSA and Azide free (AB234530)

Flow cytometric analysis of Mouse primary bone marrow cells labeling F4/80 with ab213200 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

The expression profile is consistent with the literature. CD11b+/ F4/80+ cells represent a rare population of macrophages in mouse bone marrow (PMID : 19620305 and 20625428).

Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213200).

Unconjugated

Anti-F4/80 antibody [EPR20548]
660 APC

APC Anti-F4/80 antibody [EPR20548]
578 PE

PE Anti-F4/80 antibody [EPR20548]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-F4/80 antibody [EPR20548]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-F4/80 antibody [EPR20548]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20548

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>" } } }

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Product details

ab234530 is the carrier-free version of ab213200.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature biotechnology 40:1360-1369 PubMed35449415

2022

DestVI identifies continuums of cell types in spatial transcriptomics data.

Applications

Unspecified application

Species

Unspecified reactive species

Romain Lopez,Baoguo Li,Hadas Keren-Shaul,Pierre Boyeau,Merav Kedmi,David Pilzer,Adam Jelinski,Ido Yofe,Eyal David,Allon Wagner,Can Ergen,Yoseph Addadi,Ofra Golani,Franca Ronchese,Michael I Jordan,Ido Amit,Nir Yosef

International journal of oncology 56:596-605 PubMed31894273

2020

Neutrophil extracellular traps promote liver micrometastasis in pancreatic ductal adenocarcinoma via the activation of cancer‑associated fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Shin Takesue,Kenoki Ohuchida,Tomohiko Shinkawa,Yoshiki Otsubo,Sokichi Matsumoto,Akiko Sagara,Akiko Yonenaga,Yohei Ando,Shin Kibe,Hiromichi Nakayama,Chika Iwamoto,Koji Shindo,Taiki Moriyama,Kohei Nakata,Yoshihiro Miyasaka,Takao Ohtsuka,Hiroki Toma,Yohei Tominaga,Kazuhiro Mizumoto,Makoto Hashizume,Masafumi Nakamura

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-F4/80 antibody [EPR20548] - BSA and Azide free

Flow Cytometry - Anti-F4/80 antibody [EPR20548] - BSA and Azide free (AB234530)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

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Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise