AB300421

Anti-F4/80 antibody [EPR26545-166]

Name: Anti-F4/80 antibody [EPR26545-166], macrophage marker (ab300421) | Abcam
Brand: Abcam Limited
SKU: AB300421
Price: 140 USD
Availability: InStock
Rating: 5 (3 reviews)

BOND RX™ Validated
Recombinant
Lab Essentials
20ul selling size
RabMAb
Advanced Validation
What is this?

(3 Reviews)

(91 Publications)

Anti-F4/80 antibody [EPR26545-166] (ab300421) is a rabbit monoclonal antibody detecting F4/80 in Western Blot, IHC-P, IHC-Fr, ICC/IF, mIHC. Suitable for Mouse, Rat.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

20 Images

Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling F4/80 with ab300421 at 1/500 dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on the macrophages of mouse spleen is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B : anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C : anti-CD19 staining B lymphocytes in mouse spleen.
Panel D : anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab310335, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the macrophages of rat spleen. The section was incubated with ab300421 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the Kupffer cells of rat liver. The section was incubated with ab300421 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the macrophages of mouse spleen (PMID : 19820169). The section was incubated with ab300421 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on the Kupffer cells of mouse liver (PMID : 26473015). The section was incubated with ab300421 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling F4/80 with ab300421 at 1/50 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and cytoplasmic staining in RAW264.7 cell lineNegative control : NIH/3T3 (PMID : 8550607) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

Panel A : merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse spleen.
Panel B : anti-F4/80 staining macrophages in mouse spleen.
Panel C : anti-CD19 staining B lymphocytes in mouse spleen.
Panel D : anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab300421, ab245235 and ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse spleen tissue staining with ab325921 at a 1 : 2000 (0.25 µg/ml) dilution, ab300421 anti-F4/80 used at 1 : 5000 (0.106 µg/ml) dilution and ab64088 anti-CD20 used at a 1 : 100 (0.11 µg/ml) dilution.

Panel A : anti-Thy1.2 (green; Opal™520), anti-F4/80 (magenta; Opal™690), anti-CD20 (gray; Opal™570) on mouse spleen.
Panel B : anti-Thy1.2 staining T lymphocytes in mouse spleen.
Panel C : anti-F4/80 staining macrophages in mouse spleen.
Panel D : anti-CD20 staining B lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325921, ab300421 and ab64088 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.
Panel C : anti-F4/80 staining macrophages in rat lung.
Panel D : anti-CD73 staining epithelium in rat lung.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab322404, ab300421 and ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.
Panel B : anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.
Panel C : anti-F4/80 staining macrophages in mouse lung.
Panel D : anti-CD73 staining epithelium in mouse lung.
Nuclear DNA was labeled with DAPI (shown in blue).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Lab

Immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labelling F4/80 with ab300421 at a concentration of 0.03 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with anti-rabbit HQ and anti-HQ HRP followed by a ChromoMap DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32mins.

ab300421 Anti-F4/80 antibody [EPR26545-166] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse bone marrow staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

Panel A : merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse bone marrow.
Panel B : anti-F4/80 staining macrophages in mouse bone marrow.
Panel C : anti-CD19 staining B lymphocytes in mouse bone marrow.
Panel D : anti-CD3 staining T lymphocytes in mouse bone marrow.
Nuclear DNA was labelled with DAPI (shown in blue).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

Panel A : merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse thymus.
Panel B : anti-F4/80 staining macrophages in mouse thymus.
Panel C : anti-CD19 staining B lymphocytes in mouse thymus.
Panel D : anti-CD3 staining T lymphocytes in mouse thymus.
Nuclear DNA was labelled with DAPI (shown in blue).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with ab237721 at 1/2000 ( 0.264 µg/ml) dilution.

Panel A : merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse lymph node.
Panel B : anti-F4/80 staining macrophages in mouse lymph node.
Panel C : anti-CD19 staining B lymphocytes in mouse lymph node.
Panel D : anti-CD3 staining T lymphocytes in mouse lymph node.
Nuclear DNA was labelled with DAPI (shown in blue).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

mIHC

Lab

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Supplier Data

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

All lanes:

Mouse spleen tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 160 kDa

false

Exposure time: 26s

Supplier Data

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

Lane 1:

J774A.1 (mouse reticum cell sarcoma macrophage) whole cell lysate 20

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 90 kDa,160 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution

Lane 1:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate

Lane 2:

L-929 ( mouse connective tissue fibroblast) whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 160 kDa

false

Exposure time: 15s

Carrier free

Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free)
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-F4/80 antibody [EPR26545-166]
Carrier free

Anti-F4/80 antibody [EPR26545-166] – Chicken IgY (Chimeric) – BSA and Azide Free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26545-166

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse

Applications

WB, IHC-Fr, ICC/IF, IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Not suitable for Rat.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/5000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "Not suitable for Rat.", "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/5000", "mIHC-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Anti-F4/80 antibody [EPR26545-166] (ab300421) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr IHC-P, WB and mIHC.

Abcam's high quality manufacturing and validation processes ensure Anti-F4/80 antibody [EPR26545-166] (ab300421) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-F4/80 antibody [EPR26545-166] (ab300421) specifically detects F4/80 (UniProt ID: Q61549; Molecular weight: 99kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone EPR26545-166 - ab300422.

Antibody clone EPR26545-166 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 647 (ab307470).

F4/80 is a key marker for macrophages, playing a significant role in immune response modulation and inflammation and is linked to various inflammatory diseases.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (91)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e70048 PubMed41040887

2025

Targeting the PI3K/Akt/NF-κB axis: Cluster of differentiation 5-like-mediated immunometabolic regulation of macrophage polarization in abdominal aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Hemoren Yi,Nan Liu,Zhengyang Wu,Lei Li,Tingting Li,Qixiang Liu,Man Duan,Taihu Wan

iScience 28:113519 PubMed41035689

2025

NRF2 activation in cancer cells suppresses immune infiltration into the tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Huaichun Wen,Takafumi Suzuki,Anqi Zhang,Miu Sato,Mahiro Matsumoto,Yuka Takahashi,Yushi Takahashi,Masayuki Yamamoto

Materials today. Bio 35:102299 PubMed41019489

2025

Ultrasound-activated piezoelectric scaffolds target dual Ca/NF-κB signaling pathways to orchestrate immunomodulation and osteogenesis for accelerated bone regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zheng,Pengfei Yan,Peng Liu,Chang Yan,Mengqi Zhao,Zuyong Wang,Xiaofei Zheng,Huajun Wang,Rongkai Zhang,Luke P Lee,Swee Hin Teoh

Journal of clinical and translational hepatology 13:555-567 PubMed40937080

2025

CCNE1 Promotes the Progression of Hepatic Precancerous Lesion and the Malignant Phenotype of Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Xue Hu,Lichao Yao,Wenzhi Guo

Theranostics 15:8569-8586 PubMed40860149

2025

Nanodelivery of Y-27632 by RGD-modified liposome enhances radioimmunotherapy of hepatocellular carcinoma via tumor microenvironment matrix stiffness reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Shen,Zihui Zheng,Xinyao Hu,Zhuolin Zhou,Yangtao Xu,Siyu Wang,Shuhong Yu,Xiaoqin He,Ximing Xu

BMC oral health 25:1318 PubMed40797245

2025

Identification of candidate immunity biomarkers associated with age-related variations in osteoclast activity in a mouse model of orthodontic tooth movement.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhang,Kai Yang,Lulu He,Zhen Chen,Yuhong Li,Shengfu Huang

Nature communications 16:7455 PubMed40796541

2025

Dual-targeted siRubicon delivery strategy triggers hepatocellular lipophagy for mitigating liver steatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Lan,Qiushi Li,Mingxing Yu,Xu Duan,Tao Ming,Shuo Li,Chunjiong Wang,Yi Zhu,Zhongyang Shen,Deling Kong,Yang Liu

Pharmaceutical biology 63:524-548 PubMed40719285

2025

Wen-Shen-Tong-Luo-Zhi-Tong Decoction alleviates bone loss in aged mice by suppressing LONP1-mediated macrophage senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Qinfeng Zhou,Kaixuan Wang,Cong Wang,Xiaoxian Sun,Lining Wang,Jie Sun,Yalan Pan,Muzhe Li,Zitong Zhao,Shijie Zhou,Qing Wang,Yafeng Zhang,Yong Ma,Yang Guo

Nature communications 16:6770 PubMed40695827

2025

Metabolism archetype cancer cells induce protumor TREM2 macrophages via oxLDL-mediated metabolic interplay in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tianhao Chu,Guiqi Zhu,Zheng Tang,Weifeng Qu,Rui Yang,Haiting Pan,Yi Wang,Ruilin Tian,Leilei Chen,Zhiqi Guan,Yichao Bu,Qianfu Zhao,Jiafeng Chen,Shengwei Mao,Yuan Fang,Jun Gao,Xiaoling Wu,Jian Zhou,Weiren Liu,Dan Ye,Jia Fan,Yinghong Shi

Cell death discovery 11:290 PubMed40595455

2025

Modulatory role of radioprotective 105 in mitigating oxidative stress and ferroptosis via the HO-1/SLC7A11/GPX4 axis in sepsis-mediated renal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Duo,Yanwei Yang,Jun Luo,Yumeng Cao,Qian Liu,Jiarui Zhang,Siqi Du,Jian You,Guqin Zhang,Qifa Ye,Huaqin Pan

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Anti-F4/80 antibody [EPR26545-166]

Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Western blot - Anti-F4/80 antibody [EPR26545-166] (AB300421)

Related conjugates and formulations (3)

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Immunogen

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