Name: Anti-F4/80 antibody [EPR26545-166]
SKU: ab300421
Rating: 5 (3 reviews)

Anti-F4/80 antibody EPR26545-166 is a rabbit monoclonal antibody that is used in F4/80 Western blot, IHC, ICC/IF, Flow cytometry. Suitable for Mouse, Rat samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivaled batch-batch consistency
- Validated for multiplex IHC on the Leica BOND® MAX using Opal reagents
- Validated on the Leica BOND™ RX automated IHC staining platform for F4/80 IHC

Images

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (AB300421), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC/IF	IHC-Fr	IHC-P	WB	mIHC
Mouse	Not recommended	Tested	Tested	Tested	Tested	Tested
Rat	Not recommended	Expected	Expected	Tested	Not recommended	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Not suitable for Rat.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Not suitable for Rat.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

See full target information Adgre1

Function

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

Alternative names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR26545-166
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Anti-F4/80 antibody [EPR26545-166] (ab300421) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ICC/IF, IHC-Fr IHC-P, WB and mIHC.

Abcam's high quality manufacturing and validation processes ensure Anti-F4/80 antibody [EPR26545-166] (ab300421) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-F4/80 antibody [EPR26545-166] (ab300421) specifically detects F4/80 (UniProt ID: Q61549; Molecular weight: 99kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL).

Conjugation-ready, carrier free format available for antibody clone EPR26545-166 - Anti-F4/80 antibody [EPR26545-166] (BSA and Azide free) ab300422.

Antibody clone EPR26545-166 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor^® 647 (Alexa Fluor® 647 Anti-F4/80 antibody [EPR26545-166] ab307470).

F4/80 is a key marker for macrophages, playing a significant role in immune response modulation and inflammation and is linked to various inflammatory diseases.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

Associated diseases and disorders

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat lung staining of Surfactant Protein A/PSAP with Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with Anti-CD73 antibody [EPR28214-169] ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on rat lung.

Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in rat lung.

Panel C: anti-F4/80 staining macrophages in rat lung.

Panel D: anti-CD73 staining epithelium in rat lung.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404, ab300421 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lung staining of Surfactant Protein A/PSAP with Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404 at a 1/5000 (0.092 μg/ml) dilution, F4/80 with ab300421 at 1/5000 (0.070 μg/ml) dilution, and CD73 with Anti-CD73 antibody [EPR28214-169] ab314327 at 1/500 (0.98 μg/ml), followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Surfactant Protein A/PSAP (green; Opal™ 520), anti-F4/80 (grey; Opal™ 570) and anti-CD73 (magenta; Opal™ 690) on mouse lung.

Panel B: anti-Surfactant Protein A/PSAP staining Clara cells and alveolar type II cells in mouse lung.

Panel C: anti-F4/80 staining macrophages in mouse lung.

Panel D: anti-CD73 staining epithelium in mouse lung.

Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Surfactant Protein A/PSAP antibody [EPR29097-290] ab322404, ab300421 and Anti-CD73 antibody [EPR28214-169] ab314327 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system..
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labelling F4/80 with ab300421 at a concentration of 0.03 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with anti-rabbit HQ and anti-HQ HRP followed by a ChromoMap DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH 8.5 for 32mins.
ab300421 Anti-F4/80 antibody [EPR26545-166] was incubated for 16 mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual).
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse bone marrow staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse bone marrow.
Panel B: anti-F4/80 staining macrophages in mouse bone marrow.
Panel C: anti-CD19 staining B lymphocytes in mouse bone marrow.
Panel D: anti-CD3 staining T lymphocytes in mouse bone marrow.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse spleen.
Panel B: anti-F4/80 staining macrophages in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse lymph node.
Panel B: anti-F4/80 staining macrophages in mouse lymph node.
Panel C: anti-CD19 staining B lymphocytes in mouse lymph node.
Panel D: anti-CD3 staining T lymphocytes in mouse lymph node.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus staining of F4/80 with ab300421 at a 1/5000 (0.1 µg/ml) dilution, CD19 with Anti-CD19 antibody [EPR23174-145] ab245235 at 1/1000 (0.444 µg/ml) dilution and CD3 with Anti-CD3 epsilon antibody [CAL57] ab237721 at 1/2000 ( 0.264 µg/ml) dilution.
Panel A: merged staining of anti-F4/80 (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™570) on mouse thymus.
Panel B: anti-F4/80 staining macrophages in mouse thymus.
Panel C: anti-CD19 staining B lymphocytes in mouse thymus.
Panel D: anti-CD3 staining T lymphocytes in mouse thymus.
Nuclear DNA was labelled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab300421, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse lymph node tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse spleen tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Multiplex immunohistochemistry - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse thymus tissue staining Neutrophil Elastase with Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335 at a 1/500 ( 1.076 µg/ml) dilution, F4/80 with ab300421 at a 1/5000 ( 0.106 µg/ml) dilution and NCR1 with Anti-NCR1 antibody [EPR23097-35] ab233558 at a 1/500 ( 1.114 µg/ml) dilution followed by a ready to use secondary antibody Opal Polymer HRP Ms + Rb.
Panel A: merged staining of anti-Neutrophil Elastase (magenta; Opal™690), anti-CD19 (green; Opal™520) and anti-CD3 (gray; Opal™690) on mouse spleen.Panel B: anti-Neutrophil Elastase staining neutrophils in mouse spleen.
Panel C: anti-CD19 staining B lymphocytes in mouse spleen.
Panel D: anti-CD3 staining T lymphocytes in mouse spleen.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of Anti-Neutrophil Elastase antibody [EPR28386-66] ab310335, Anti-CD19 antibody [EPR23174-145] ab245235 and Anti-CD3 epsilon antibody [CAL57] ab237721 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on the Kupffer cells of rat liver.The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on the Kupffer cells of mouse liver (PMID: 26473015).The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Frozen sections) - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling F4/80 with ab300421 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on the macrophages of mouse spleen is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling F4/80 with ab300421 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing membranous and cytoplasmic staining in RAW264.7 cell lineNegative control: NIH/3T3 (PMID: 8550607) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.
Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution
All lanes: Mouse spleen tissue lysate 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 160 kDa
Exposure time: 26s
Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution
Lane 1: RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate
Lane 2: L-929 ( mouse connective tissue fibroblast) whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 160 kDa
Exposure time: 15s
Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-F4/80 antibody [EPR26545-166] (ab300421) at 1/1000 dilution
Lane 1: J774A.1 (mouse reticum cell sarcoma macrophage) whole cell lysate 20
Lane 2: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 90 kDa, 160 kDa
Exposure time: 15s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on the macrophages of rat spleen.The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-F4/80 antibody [EPR26545-166] (ab300421)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling F4/80 with ab300421 at 1/5000 (0.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on the macrophages of mouse spleen (PMID: 19820169).The section was incubated with abxxxxxx for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins