Anti-F4/80 antibody [F4/80] - Macrophage Marker is a rat monoclonal antibody that is used to detect F4/80 in ICC/IF. Suitable for Mouse samples.
- Macrophage marker
- Cited in over 30 publications
Images
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (AB90247), expandable thumbnail](https://content.abcam.com/products/images/f4-80-antibody-f4-80-macrophage-marker-ab90247--immunocytochemistry-immunofluorescence-img416064.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (AB90247)")
Publications
Filter by
- International journal of biological sciences 19:1123-11452023Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian ZhuPubMed 36923940
- Nature immunology 23:518-5312022Neutrophils direct preexisting matrix to initiate repair in damaged tissues.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdrian Fischer,Juliane Wannemacher,Simon Christ,Tim Koopmans,Safwen Kadri,Jiakuan Zhao,Mahesh Gouda,Haifeng Ye,Martin Mück-Häusl,Peter W Krenn,Hans-Günther Machens,Reinhard Fässler,Philipp-Alexander Neumann,Stefanie M Hauck,Yuval RinkevichPubMed 35354953
- Nature metabolism 3:1706-17262021The flavonoid procyanidin C1 has senotherapeutic activity and increases lifespan in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQixia Xu,Qiang Fu,Zi Li,Hanxin Liu,Ying Wang,Xu Lin,Ruikun He,Xuguang Zhang,Zhenyu Ju,Judith Campisi,James L Kirkland,Yu SunPubMed 34873338
- Nature communications 11:56532020Injury triggers fascia fibroblast collective cell migration to drive scar formation through N-cadherin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongsheng Jiang et. al.PubMed 33159076
- Reproductive sciences (Thousand Oaks, Calif.) 28:693-7022020Construction and Optimization of an Endometrial Injury Model in Mice by Transcervical Ethanol Perfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenghui Zhang,Yuliang Sun,Dongli Jiang,Tongtong Chen,Ruihong Liu,Xinyi Li,Yilin Lu,Liang Qiao,Ying Pan,Yanli Liu,Juntang LinPubMed 32939736
- International journal of molecular sciences 21:2020Impaired ECM Remodeling and Macrophage Activity Define Necrosis and Regeneration Following Damage in Aged Skeletal Muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFasih Ahmad Rahman et. al.PubMed 32605082
- Journal of colloid and interface science 549:50-622019Molybdenum disulfide nanoflowers mediated anti-inflammation macrophage modulation for spinal cord injury treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuodong Sun et. al.PubMed 31015056
- Oral diseases 25:265-2732018Macrophage polarization in human gingival tissue in response to periodontal disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Na Zhou,Chun-Sheng Bi,Li-Na Gao,Ying An,Fang Chen,Fa-Ming ChenPubMed 30285304
- Nature medicine 24:1246-12562018Senolytics improve physical function and increase lifespan in old age.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Xu et. al.PubMed 29988130
- Stem cells international 2018:46585032018Injected Human Muscle Precursor Cells Overexpressing PGC-1 Enhance Functional Muscle Regeneration after Trauma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeana Haralampieva et. al.PubMed 29531537
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Mouse | Tested |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-10.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.
Alternative names
Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor
Recommended products
Anti-F4/80 antibody [F4/80] - Macrophage Marker is a rat monoclonal antibody that is used to detect F4/80 in ICC/IF. Suitable for Mouse samples.
- Macrophage marker
- Cited in over 30 publications
Key facts
- Isotype
- IgG2b
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- F4/80
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) is a rat monoclonal antibody and is validated for use in ICC/IF in human, mouse samples.
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.
Abcam's high quality validation processes ensure Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has high sensitivity and specificity.
Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 95kDa) and is sold in 100 µg selling sizes.
Antibody clone F4/80 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (Alexa Fluor® 488 Anti-F4/80 antibody [F4/80] ab204266, Alexa Fluor® 647 Anti-F4/80 antibody [F4/80] ab204467).
F4/80 is a marker for murine macrophages, playing a key role in immune responses and inflammation, particularly in diseases like cancer and chronic inflammatory conditions. F4/80 is a key biomarker in chronic inflammatory conditions.
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.
- Biological function summary
F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.
- Pathways
F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.
- Associated diseases and disorders
F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247), expandable thumbnail](https://content.abcam.com/products/images/f4-80-antibody-f4-80-macrophage-marker-ab90247--immunocytochemistry-immunofluorescence-img416064.jpg "Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)")
Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247)
ab90247 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab90247 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com