AB90247

Anti-F4/80 antibody [F4/80] - Macrophage Marker

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(41 Publications)

Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) is a rat monoclonal antibody detecting F4/80 in ICC/IF. Suitable for Mouse.

- Over 30 publications

View Alternative Names

Emr1, Gpf480, Adgre1, Adhesion G protein-coupled receptor E1, Cell surface glycoprotein F4/80, EGF-like module receptor 1, EGF-like module-containing mucin-like hormone receptor-like 1, EMR1 hormone receptor

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (AB90247)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (AB90247)

ab90247 staining F4/80 in Raw264.7 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab90247 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-F4/80 antibody [F4/80]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-F4/80 antibody [F4/80]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

F4/80

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) is a rat monoclonal antibody and is validated for use in ICC/IF in human, mouse samples.

Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has been cited over 31 times in peer reviewed journals and is trusted by the scientific community.

Abcam's high quality validation processes ensure Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) has high sensitivity and specificity.

Anti-F4/80 antibody [F4/80] - Macrophage Marker (ab90247) specifically detects F4/80 (UniProt ID: Q14246; Molecular weight: 95kDa) and is sold in 100 µg selling sizes.

Antibody clone F4/80 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647 (ab204266, ab204467).

F4/80 is a marker for murine macrophages, playing a key role in immune responses and inflammation, particularly in diseases like cancer and chronic inflammatory conditions. F4/80 is a key biomarker in chronic inflammatory conditions.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

F4/80 also known as Emr1 (Epidermal growth factor module-containing mucin-like hormone receptor 1) is a well-characterized surface protein with a mass of approximately 160 kDa. This protein is recognized as a marker for murine macrophages. F4/80 is heavily expressed on cells of the monocyte and macrophage lineage particularly in tissue-resident macrophages found in organs like the liver spleen and lung. Its expression is vital for identifying macrophages in various types of immunological studies including F4/80 staining and macrophage marker analysis.

Biological function summary

F4/80 plays a role in modulating the immune response by interacting with other cell surface molecules. While not part of a larger protein complex F4/80 influences macrophage functions such as phagocytosis and cytokine production. Its expression contributes to the regulation of tissue homeostasis and inflammation. The protein is critical in maintaining the macrophage population required for reliable immune surveillance and response to pathogens.

Pathways

F4/80 is an integral part of the immunological and inflammatory signaling pathways. It interacts with TLRs (Toll-like receptors) and other cell surface molecules that sense microbial components therefore helping in the initiation of innate immune responses. F4/80 is connected to proteins like cytokine receptors which play roles in mediating and resolving inflammatory signals important for maintaining balanced immune activity.

F4/80 is associated with conditions like chronic inflammation and autoimmune disorders. Its expression levels can often correlate with disease state impacting diseases such as rheumatoid arthritis and liver fibrosis. In these conditions F4/80 interacts with proteins like TNF-alpha and IL-1beta which are involved in inflammatory processes. Understanding F4/80's role can help uncover therapeutic targets aimed at modulating macrophage functions to treat these disorders effectively.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Orphan receptor involved in cell adhesion and probably in cell-cell interactions specifically involving cells of the immune system. May play a role in regulatory T-cells (Treg) development.

See full target information Adgre1

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Publications (41)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:29 PubMed40793857

2025

Inhibiting The uPA/uPAR Pathway Affords Photoreceptor Resilience and Preserves Retinal Function in a Mouse Model of Retinitis Pigmentosa.

Applications

Unspecified application

Species

Unspecified reactive species

Rosario Amato,Alessio Canovai,Alberto Melecchi,Maria De Fenza,Linda Leone,Vincenzo Pavone,Daniele D'Alonzo,Maurizio Cammalleri,Massimo Dal Monte

MedComm 6:e70276 PubMed40692664

2025

Long-Term High-Fat Diet Affected Bone Marrow Microenvironment During Aging at Single-Cell Resolution.

Applications

Unspecified application

Species

Unspecified reactive species

Yidan Pang,Siyuan Zhu,Peng Ding,Senyao Zhang,Yi Zhang,Fang Ye,Changqing Zhang,Junjie Gao,Jimin Yin

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409086 PubMed39804990

2025

TIA1-Mediated Stress Granules Promote the Neuroinflammation and Demyelination in Experimental Autoimmune Encephalomyelitis through Upregulating IL-31RA Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Hua,Lingting Jin,Zheyu Fang,Yiyun Weng,Yuan Zhang,Jingjing Zhang,Dewei Xie,Yang Tang,Siyu Guo,Yingying Huang,Yilin Dai,Jia Li,Zhihui Huang,Xu Zhang

Nature communications 15:10138 PubMed39604430

2024

LungVis 1.0: an automatic AI-powered 3D imaging ecosystem unveils spatial profiling of nanoparticle delivery and acinar migration of lung macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Qiongliang Liu,Pramod Kumar,Arunima Sengupta,Ali Farnoud,Ruolin Shen,Darya Trofimova,Sebastian Ziegler,Neda Davoudi,Ali Doryab,Ali Önder Yildirim,Markus E Diefenbacher,Herbert B Schiller,Daniel Razansky,Marie Piraud,Gerald Burgstaller,Wolfgang G Kreyling,Fabian Isensee,Markus Rehberg,Tobias Stoeger,Otmar Schmid

Science signaling 17:eadn8936 PubMed39078919

2024

Peripheral macrophages contribute to nociceptor priming in mice with chronic intermittent hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel B Chivers,Mary Ann Andrade,Robert J Hammack,John Shannonhouse,Ruben Gomez,Yan Zhang,Brian Nguyen,Pankil Shah,Yu Shin Kim,Glenn M Toney,Nathaniel A Jeske

Bioactive materials 39:443-455 PubMed38873087

2024

Mechanically skin-like and water-resistant self-healing bioelastomer for high-tension wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyi Huang,Hongying Chen,Zenghui Jia,Xingqi Song,Sinan Wang,Baoshuai Bai,Jian Wang,Junfeng Zhang,Guangdong Zhou,Dong Lei

Cell host & microbe 32:411-424.e10 PubMed38307020

2024

Anaerobic respiration of host-derived methionine sulfoxide protects intracellular Salmonella from the phagocyte NADPH oxidase.

Applications

Unspecified application

Species

Unspecified reactive species

Ju-Sim Kim,Lin Liu,Sashi Kant,David J Orlicky,Siva Uppalapati,Alyssa Margolis,Bennett J Davenport,Thomas E Morrison,Jennifer Matsuda,Michael McClelland,Jessica Jones-Carson,Andres Vazquez-Torres

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 72:1485-1500 PubMed37335321

2023

A positive feedback cycle between the alarmin S100A8/A9 and NLRP3 inflammasome-GSDMD signalling reinforces the innate immune response in Candida albicans keratitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolong Fang,Huifang Lian,Shuang Liu,Jingcun Dong,Xia Hua,Wenguang Li,Chunyang Liao,Xiaoyong Yuan

International journal of biological sciences 19:1123-1145 PubMed36923940

2023

Low-intensity pulsed ultrasound promotes skeletal muscle regeneration via modulating the inflammatory immune microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Haocheng Qin,Zhiwen Luo,Yaying Sun,Zhong He,Beijie Qi,Yisheng Chen,Junlong Wang,Ce Li,Weiwei Lin,Zhihua Han,Yulian Zhu

Oral diseases 30:769-779 PubMed36097830

2022

Weakening of M1 macrophage and bone resorption in periodontitis cystathionine γ-lyase-deficient mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sijin Li,Yongmei Hua,Chongshan Liao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-F4/80 antibody [F4/80] - Macrophage Marker

Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [F4/80] - Macrophage Marker (AB90247)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (41)

Product promise