AB128917

Anti-FAAH1 antibody [EPR7549]

RabMAb
Recombinant
What is this?

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(7 Publications)

Rabbit Recombinant Monoclonal FAAH1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 7 publications.

View Alternative Names

FAAH1, FAAH, Fatty-acid amide hydrolase 1, Anandamide amidohydrolase 1, Fatty acid ester hydrolase, Oleamide hydrolase 1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (AB128917)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (AB128917)

ab128917 at 1/250 dilution staining FAAH1 in paraffin embedded Human brain tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (AB128917)

ab128917 at 1/250 dilution staining FAAH1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-FAAH1 antibody [EPR7549] (AB128917)

All lanes:

Western blot - Anti-FAAH1 antibody [EPR7549] (ab128917) at 1/1000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Lane 3:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated at 1/2000 dilution

Predicted band size: 63 kDa

false

OI-RD Scanning - Anti-FAAH1 antibody [EPR7549] (AB128917)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-FAAH1 antibody [EPR7549] (AB128917)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-FAAH1 antibody [EPR7549] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7549

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "Antigen retrieval is highly recommended.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty acid amide hydrolase 1 (FAAH1) often referred to simply as FAAH is an enzyme that plays a critical role in the degradation of fatty acid amides such as anandamide in the body. This enzyme weighing approximately 63 kDa is a part of the amidase signature family and is characterized by its amidase hydrolase activity. FAAH1 is predominantly expressed in the nervous system liver and gastrointestinal tract where it hydrolyses amide bonds facilitating the termination of signaling pathways initiated by neurotransmitter-like fatty acid amides.

Biological function summary

FAAH1 is essential for maintaining homeostasis by modulating levels of bioactive lipids. It exerts control over endocannabinoid signaling by breaking down anandamide a lipid derivative functioning as a neurotransmitter. FAAH1 does not act as part of a larger protein complex. Instead it operates alone to regulate the duration and intensity of signaling rather than relying on direct interactions within a complex. The enzyme's function impacts the neurochemical milieu in tissues where it is actively expressed thereby affecting mood pain sensation and more.

Pathways

FAAH1 has a significant role in the endocannabinoid signaling pathway and fatty acid metabolism. Within these pathways it modulates signaling by hydrolysing bioactive amides like anandamide influencing overall lipid signaling. FAAH1 activities intersect with several other proteins such as cannabinoid receptors which bind the substrates that FAAH1 eventually degrades. These interactions are vital in controlling signal transduction processes that regulate physiological responses including stress and analgesia.

FAAH1 has been implicated in conditions like chronic pain and anxiety disorders. Altered FAAH1 function or expression can lead to dysregulation of endocannabinoid signaling contributing to the pathophysiology of these conditions. The enzyme's modulation of anandamide levels connects it to proteins such as CB1 and CB2 cannabinoid receptors which mediate the effects of endocannabinoids in neurological and psychiatric disorders. Research into FAAH1 inhibitors as therapeutic agents highlights its importance in potential treatments for managing pain and anxiety.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of endogenous amidated lipids like the sleep-inducing lipid oleamide ((9Z)-octadecenamide), the endocannabinoid anandamide (N-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-ethanolamine), as well as other fatty amides, to their corresponding fatty acids, thereby regulating the signaling functions of these molecules (PubMed : 17015445, PubMed : 19926788, PubMed : 9122178). Hydrolyzes polyunsaturated substrate anandamide preferentially as compared to monounsaturated substrates (PubMed : 17015445, PubMed : 9122178). It can also catalyze the hydrolysis of the endocannabinoid 2-arachidonoylglycerol (2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol) (PubMed : 21049984). FAAH cooperates with PM20D1 in the hydrolysis of amino acid-conjugated fatty acids such as N-fatty acyl glycine and N-fatty acyl-L-serine, thereby acting as a physiological regulator of specific subsets of intracellular, but not of extracellular, N-fatty acyl amino acids (By similarity).

See full target information FAAH

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 10:12 PubMed38184644

2024

Fatty acid amide hydrolase drives adult mammary gland development by promoting luminal cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Tundidor,Marta Seijo-Vila,Sandra Blasco-Benito,María Rubert-Hernández,Gema Moreno-Bueno,Laura Bindila,Rubén Fernández de la Rosa,Manuel Guzmán,Cristina Sánchez,Eduardo Pérez-Gómez

Nature communications 14:3130 PubMed37253733

2023

Identification of fatty acid amide hydrolase as a metastasis suppressor in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Isabel Tundidor,Marta Seijo-Vila,Sandra Blasco-Benito,María Rubert-Hernández,Sandra Adámez,Clara Andradas,Sara Manzano,Isabel Álvarez-López,Cristina Sarasqueta,María Villa-Morales,Carmen González-Lois,Esther Ramírez-Medina,Belén Almoguera,Antonio J Sánchez-López,Laura Bindila,Sigrid Hamann,Norbert Arnold,Christoph Röcken,Ignacio Heras-Murillo,David Sancho,Gema Moreno-Bueno,María M Caffarel,Manuel Guzmán,Cristina Sánchez,Eduardo Pérez-Gómez

Frontiers in cellular neuroscience 16:902278 PubMed36003139

2022

-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

R Scott Duncan,Sean M Riordan,Conner W Hall,Andrew J Payne,Kent D Chapman,Peter Koulen

Oncology letters 20:277 PubMed33014155

2020

MicroRNA-1297 downregulation inhibits breast cancer cell epithelial-mesenchymal transition and proliferation in a FA2H-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Li,Bin Lian,Yaobang Liu,Dahai Chai,Jinping Li

Open medicine (Warsaw, Poland) 15:882-889 PubMed33344772

2020

miR-300/FA2H affects gastric cancer cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Jie Li,Chunxiao Huang,Tao Huang,Mengpei Zhang,Lijiang Huang

The Journal of clinical investigation 129:2333-2350 PubMed31063987

2019

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Zhang,Qi Yang,Le Yang,Yan-Jiao Li,Xin-Shang Wang,Yu-Jiao Li,Rui-Li Dang,Shao-Yu Guan,Yan-Yan Guo,Ting Sun,Yu-Mei Wu,An Liu,Yan Zhang,Shui-Bing Liu,Ming-Gao Zhao

Cell metabolism 25:1334-1347.e4 PubMed28591636

2017

N-acyl Taurines and Acylcarnitines Cause an Imbalance in Insulin Synthesis and Secretion Provoking β Cell Dysfunction in Type 2 Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela Aichler,Daniela Borgmann,Jan Krumsiek,Achim Buck,Patrick E MacDonald,Jocelyn E Manning Fox,James Lyon,Peter E Light,Susanne Keipert,Martin Jastroch,Annette Feuchtinger,Nikola S Mueller,Na Sun,Andrew Palmer,Theodore Alexandrov,Martin Hrabe de Angelis,Susanne Neschen,Matthias H Tschöp,Axel Walch

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FAAH1 antibody [EPR7549]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (AB128917)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAAH1 antibody [EPR7549] (AB128917)

Western blot - Anti-FAAH1 antibody [EPR7549] (AB128917)

OI-RD Scanning - Anti-FAAH1 antibody [EPR7549] (AB128917)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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