Rabbit Recombinant Monoclonal FABP-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunoprecipitation-img3683.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106415.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93260.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129335.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)")
![Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunoprecipitation-img68070.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)")
Publications
- Biomedicine & pharmacotherapy = Biomedecine & phar 118:1092512019PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhao et. al.PubMed 31351426
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Expected |
| Mouse | Expected | Not recommended | Expected | Tested |
| Rat | Predicted | Not recommended | Expected | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Version
4 products for Alternative Product
Target data
Function
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Alternative names
mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm, GOT2, KYAT4
Recommended products
Rabbit Recombinant Monoclonal FABP-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Alternative names
mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm, GOT2, KYAT4
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR12354(B)
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab240164 is the carrier-free version of Anti-FABP-1 antibody [EPR12354(B)] ab171739.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.
- Biological function summary
FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.
- Pathways
FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.
- Associated diseases and disorders
FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunoprecipitation-img3683.jpg "Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Anti-FABP-1 antibody [EPR12354(B)] ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
Lane 1 (input): Human fetal liver lysate 10 µg
Lane 2 (+): Anti-FABP-1 antibody [EPR12354(B)] ab171739 & Human fetal liver lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-FABP-1 antibody [EPR12354(B)] ab171739 in Human fetal liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739)All lanes: Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (Anti-FABP-1 antibody [EPR12354(B)] ab171739)
Predicted band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106415.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified Anti-FABP-1 antibody [EPR12354(B)] ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93260.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified Anti-FABP-1 antibody [EPR12354(B)] ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129335.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified Anti-FABP-1 antibody [EPR12354(B)] ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739)
![Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunoprecipitation-img68070.jpg "Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using Anti-FABP-1 antibody [EPR12354(B)] ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2: 1X PBS (negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739).
All lanes: Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (Anti-FABP-1 antibody [EPR12354(B)] ab171739)
Predicted band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82488.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using Anti-FABP-1 antibody [EPR12354(B)] ab171739 (unpurified) at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58022.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)
Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using Anti-FABP-1 antibody [EPR12354(B)] ab171739 (unpurified) at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP-1 antibody [EPR12354(B)] ab171739).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82488.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12354b-bsa-and-azide-free-ab240164--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58022.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (ab240164)")
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