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AB240164

Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free

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(2 Publications)

Rabbit Recombinant Monoclonal FABP-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

View Alternative Names

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IP

Supplier Data

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2 : 1X PBS (negative control).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).

All lanes:

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/en-us/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)

Predicted band size: 48 kDa

false

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IP

Unknown

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
Lane 1 (input) : Human fetal liver lysate 10 µg
Lane 2 (+) : ab171739 & Human fetal liver lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab171739 in Human fetal liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

All lanes:

Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/en-us/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)

Predicted band size: 48 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)

  • Unconjugated

    Anti-FABP-1 antibody [EPR12354(B)]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-FABP-1 antibody [EPR12354(B)]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-FABP-1 antibody [EPR12354(B)]

  • 660 APC

    APC Anti-FABP-1 antibody [EPR12354(B)]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-FABP-1 antibody [EPR12354(B)]

  • 578 PE

    PE Anti-FABP-1 antibody [EPR12354(B)]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-FABP-1 antibody [EPR12354(B)]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-FABP-1 antibody [EPR12354(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12354(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

ab240164 is the carrier-free version of ab171739.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.
Biological function summary

FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.

Pathways

FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.

FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
See full target information GOT2

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750

2023

Electroacupuncture alleviates water avoidance stress-induced irritable bowel syndrome in mice by improving intestinal barrier functions and suppressing the expression of inflammatory cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Mengzhu,Zhang Yujie,Song Yafang,Guo Jing,Wang Yuhang,Xin Chen,G U Dongmei,Sun Jianhua,Pei Lixia

Biomedicine & pharmacotherapy = Biomedecine & phar 118:109251 PubMed31351426

2019

PPAR signaling pathway in the first trimester placenta from in vitro fertilization and embryo transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Xiuli Zheng,Jingfang Liu,Rong Zheng,Rui Yang,Ying Wang,Lifang Sun
View all publications

Product promise

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