Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free
- RabMAb
- Recombinant
- What is this?
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(2 Publications)
Rabbit Recombinant Monoclonal FABP-1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
View Alternative Names
KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Immunohistochemistry of paraffin-embedded Human urinary bladder carcinoma tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Immunohistochemistry of paraffin-embedded Human liver tissue labeling FABP-1 using ab171739 (unpurified) at 1/50 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).
- IP
Supplier Data
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Western blot analysis on immunoprecipitation pellet from Human fetal liver lysate (lane 1) labeling FABP-1 using ab171739 (unpurified) at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG. Lane 2 : 1X PBS (negative control).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739).
All lanes:
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/en-us/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)
Predicted band size: 48 kDa
false
- IP
Unknown
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
ab171739 (purified ) at 1/50 dilution (2 µg) immunoprecipitating FABP-1 in Human fetal liver lysate.
Lane 1 (input) : Human fetal liver lysate 10 µg
Lane 2 (+) : ab171739 & Human fetal liver lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab171739 in Human fetal liver lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)
All lanes:
Immunoprecipitation - Anti-FABP-1 antibody [EPR12354(B)] (<a href='/en-us/products/primary-antibodies/fabp-1-antibody-epr12354b-ab171739'>ab171739</a>)
Predicted band size: 48 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12354(B)] - BSA and Azide free (AB240164)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue sections labeling FABP-1 with purified ab171739 at 1/4000 dilution (0.25 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab171739)
Related conjugates and formulations (8)
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Anti-FABP-1 antibody [EPR12354(B)]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-FABP-1 antibody [EPR12354(B)]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-FABP-1 antibody [EPR12354(B)]
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660 APC
APC Anti-FABP-1 antibody [EPR12354(B)]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-FABP-1 antibody [EPR12354(B)]
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578 PE
PE Anti-FABP-1 antibody [EPR12354(B)]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-FABP-1 antibody [EPR12354(B)]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-FABP-1 antibody [EPR12354(B)]
Reactivity data
Product details
ab240164 is the carrier-free version of ab171739.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.
Pathways
FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.
Product protocols
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Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:494-500 PubMed37147750
2023
Applications
Unspecified application
Species
Unspecified reactive species
Biomedicine & pharmacotherapy = Biomedecine & phar 118:109251 PubMed31351426
2019
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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