AB180162

Anti-FABP-1 antibody [EPR12355(B)]

RabMAb
Recombinant
What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal FABP-1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm

4 Images

Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Intracellular flow cytometric analysis of permeabilized HeLa cells labeling FABP-1 using ab180162 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling FABP-1 using ab180162 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling FABP-1 using ab180162 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

All lanes:

Western blot - Anti-FABP-1 antibody [EPR12355(B)] (ab180162) at 1/5000 dilution

Lane 1:

Fetal liver tissue lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 48 kDa

true

Carrier free

Anti-FABP-1 antibody [EPR12355(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12355(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "The use of an HRP/AP polymerized secondary antibody is recommended.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.

Biological function summary

FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.

Pathways

FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.

FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.

See full target information GOT2

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40643519

2025

Short-Term Incubation of H9c2 Cardiomyocytes with Cannabigerol Attenuates Diacylglycerol Accumulation in Lipid Overload Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Sylwia Dziemitko,Adrian Chabowski,Ewa Harasim-Symbor

Scientific reports 13:3694 PubMed36879113

2023

Cannabidiol improves muscular lipid profile by affecting the expression of fatty acid transporters and inhibiting de novo lipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Patrycja Bielawiec,Sylwia Dziemitko,Karolina Konstantynowicz-Nowicka,Adrian Chabowski,Janusz Dzięcioł,Ewa Harasim-Symbor

Cells 11: PubMed35563741

2022

The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?

Applications

Unspecified application

Species

Unspecified reactive species

Agnieszka Mikłosz,Bartłomiej Łukaszuk,Elżbieta Supruniuk,Kamil Grubczak,Aleksandra Starosz,Magdalena Kusaczuk,Monika Naumowicz,Adrian Chabowski

Theranostics 11:7262-7275 PubMed34158849

2021

Congenital biliary atresia is correlated with disrupted cell junctions and polarity caused by Cdc42 insufficiency in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjie Zhou,Hongjie Ji,Qing Xu,Xiaoyun Zhang,Xiaoyue Cao,Yuwei Chen,Mingyang Shao,Zhenru Wu,Jie Zhang,Changli Lu,Jiayin Yang,Yujun Shi,Hong Bu

Experimental physiology 106:1208-1223 PubMed33675111

2021

Resting skeletal muscle PNPLA2 (ATGL) and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver J Chrzanowski-Smith,Robert M Edinburgh,Eleanor Smith,Mark P Thomas,Jean-Philippe Walhin,Francoise Koumanov,Sean Williams,James A Betts,Javier T Gonzalez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FABP-1 antibody [EPR12355(B)]

Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Western blot - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise