Rabbit Recombinant Monoclonal FABP-1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-FABP-1 antibody [EPR12355(B)] (AB180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--western-blot-img116226.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108130.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93181.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)")
![Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--flow-cytometry-intracellular-img79108.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (AB180162)")
Publications
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- Scientific reports 13:36942023Cannabidiol improves muscular lipid profile by affecting the expression of fatty acid transporters and inhibiting de novo lipogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrycja Bielawiec,Sylwia Dziemitko,Karolina Konstantynowicz-Nowicka,Adrian Chabowski,Janusz Dzięcioł,Ewa Harasim-SymborPubMed 36879113
- Cells 11:2022The Phenotype of the Adipocytes Derived from Subcutaneous and Visceral ADMSCs Is Altered When They Originate from Morbidly Obese Women: Is There a Memory Effect?Applications:Unspecified applicationReactive species:Unspecified reactive speciesAgnieszka Mikłosz,Bartłomiej Łukaszuk,Elżbieta Supruniuk,Kamil Grubczak,Aleksandra Starosz,Magdalena Kusaczuk,Monika Naumowicz,Adrian ChabowskiPubMed 35563741
- Experimental physiology 106:1208-12232021Resting skeletal muscle PNPLA2 (ATGL) and CPT1B are associated with peak fat oxidation rates in men and women but do not explain observed sex differences.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOliver J Chrzanowski-Smith,Robert M Edinburgh,Eleanor Smith,Mark P Thomas,Jean-Philippe Walhin,Francoise Koumanov,Sean Williams,James A Betts,Javier T GonzalezPubMed 33675111
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes The use of an HRP/AP polymerized secondary antibody is recommended. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids.
Alternative names
KYAT4, GOT2, mAspAT, Fatty acid-binding protein, Glutamate oxaloacetate transaminase 2, Kynurenine aminotransferase 4, Kynurenine aminotransferase IV, Kynurenine--oxoglutarate transaminase 4, Kynurenine--oxoglutarate transaminase IV, Plasma membrane-associated fatty acid-binding protein, Transaminase A, FABP-1, FABPpm
Recommended products
Rabbit Recombinant Monoclonal FABP-1 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12355(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Fatty Acid Binding Protein 1 (FABP-1) also known as Liver-type fatty acid-binding protein (L-FABP) is a small protein with a molecular weight of approximately 14 kDa. FABP-1 is abundantly expressed in the liver and also found in the intestine and kidneys. It functions by binding long-chain fatty acids and other lipophilic substances facilitating their transport within cells. The protein aids intracellular lipid trafficking assisting in the storage and metabolism of fatty acids.
- Biological function summary
FABP-1 assists cells in maintaining lipid homeostasis by transporting these fatty acids to different cellular compartments for oxidation signaling or storage. It does not form part of a complex but independently interacts with lipids to buffer their concentration within the cytoplasm. Its role in lipid transport is essential for maintaining metabolic balance and energy production.
- Pathways
FABP-1 participates in key metabolic pathways specifically the lipid metabolism and peroxisome proliferator-activated receptor (PPAR) signaling pathway. These pathways influence energy homeostasis and lipid modulation within cells. FABP-1 interacts with other proteins such as peroxisome proliferator-activated receptor alpha (PPARα) which regulates the expression of genes involved in lipid metabolic processes.
- Associated diseases and disorders
FABP-1 shows connections to liver conditions and metabolic syndrome. Altered levels of FABP-1 can indicate liver injury and could be used as a biomarker for non-alcoholic fatty liver disease (NAFLD). FABP-1 also connects to apolipoprotein E (ApoE) a protein involved in lipid metabolism in relation to the development and progression of atherosclerosis impacting cardiovascular health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-FABP-1 antibody [EPR12355(B)] (ab180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--western-blot-img116226.jpg "Western blot - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)")
Western blot - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)
All lanes: Western blot - Anti-FABP-1 antibody [EPR12355(B)] (ab180162) at 1/5000 dilution
Lane 1: Fetal liver tissue lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HepG2 cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Developed using the ECL technique.
Predicted band size: 48 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108130.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling FABP-1 using ab180162 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93181.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling FABP-1 using ab180162 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162), expandable thumbnail](https://content.abcam.com/products/images/fabp-1-antibody-epr12355b-ab180162--flow-cytometry-intracellular-img79108.jpg "Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)")
Flow Cytometry (Intracellular) - Anti-FABP-1 antibody [EPR12355(B)] (ab180162)
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling FABP-1 using ab180162 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
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