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AB155089

Anti-FABP12 antibody [EPR10536]

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(1 Publication)

Rabbit Recombinant Monoclonal FABP12 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

Fatty acid-binding protein 12, FABP12

3 Images
Flow Cytometry (Intracellular) - Anti-FABP12 antibody [EPR10536] (AB155089)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-FABP12 antibody [EPR10536] (AB155089)

Intracellular flow cytometric analysis of permeabilized Jurkat cells, labeling FABP12 with ab155089 at 1/10 dilution (red) compared with a Rabbit IgG control (green).

Immunocytochemistry/ Immunofluorescence - Anti-FABP12 antibody [EPR10536] (AB155089)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FABP12 antibody [EPR10536] (AB155089)

Immunofluorescent analysis of Jurkat cells labeling FABP12 with ab155089 at 1/50 dilution.

Western blot - Anti-FABP12 antibody [EPR10536] (AB155089)
  • WB

Unknown

Western blot - Anti-FABP12 antibody [EPR10536] (AB155089)

All lanes:

Western blot - Anti-FABP12 antibody [EPR10536] (ab155089) at 1/1000 dilution

Lane 1:

Rat eyeball tissue lysate at 10 µg

Lane 2:

Mouse eyeball lysate at 10 µg

Lane 3:

Human fetal brain tissue lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10536

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FABP12 also known as fatty acid-binding protein 12 plays a role in lipid transport and metabolism. This protein facilitates the binding and transport of long-chain fatty acids within cells. Although its exact molecular weight is not universally established similar fatty acid-binding proteins often have masses around 14-15 kDa. FABP12 expression primarily occurs in the mammalian retina with detectable levels in other tissues including certain types of cancer tissues.
Biological function summary

FABP12 influences cellular processes through its interaction with lipids. This protein contributes to fatty acid uptake and intracellular lipid homeostasis. It does not function as part of a larger macromolecular complex but interacts dynamically with fatty acids impacting lipid signaling and energy balance within the cell. Through these interactions FABP12 supports cellular energy production and lipid storage regulation.

Pathways

FABP12 is involved in lipid metabolism and energy homeostasis pathways. One important pathway includes the peroxisome proliferator-activated receptor (PPAR) signaling pathway which regulates lipid metabolism. Nearby proteins like PPAR-alpha and PPAR-gamma are important for FABP12’s regulation of lipid-derived energy. Another relevant connection is to the retinoic acid signaling pathway where FABP12’s binding affinity with retinoids potentially influences cellular differentiation and development.

Research connects FABP12 to cancer and metabolic syndromes. In some cancers altered expression levels of FABP12 appear to influence tumor growth and metabolism. Studies have linked FABP12 with proteins such as FABP5 another member of the fatty acid-binding protein family in cancerous tissue dynamics. Additionally FABP12 involvement in metabolic disorders suggests a relationship with insulin resistance where its expression may impact the function of key proteins related to glucose metabolism.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in lipid transport.
See full target information Fatty acid-binding protein 12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of neurochemistry 168:2479-2494 PubMed38497582

2024

Chronic social defeat stress induces the down-regulation of the Nedd4L-GLT-1 ubiquitination pathway in the prefrontal cortex of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Aika Kosuge,Kazuo Kunisawa,Tsubasa Iida,Bolati Wulaer,Tomoki Kawai,Moeka Tanabe,Kuniaki Saito,Toshitaka Nabeshima,Akihiro Mouri
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com