Anti-FABP4 antibody [9B8D]

• Flow Cyt

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Flow Cytometry - Anti-FABP4 antibody [9B8D] (AB93945)

Overlay histogram showing THP1 cells stained with ab93945 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93945, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

• WB

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Western blot - Anti-FABP4 antibody [9B8D] (AB93945)

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Western blot - Anti-FABP4 antibody [9B8D] (ab93945) at 1/500 dilution

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FABP4-hIgGFc transfected HEK293 cell lysate

Predicted band size: 15 kDa

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