Anti-FABP4 antibody [EPR3579]

• mIHC

Lab

Multiplex immunohistochemistry - Anti-FABP4 antibody [EPR3579] (AB92501)

Fluorescence multiplex immunohistochemical analysis of the human breast (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-B7H4 (ab252438, red; Opal™690), anti-CD10 (ab255609, gray; Opal™520) and anti-FABP4 (ab92501, cyan; Opal™570) on human breast. Panel B : anti-B7H4 stained on glandular lumens. Panel C : anti-CD10 stained on myoepithelial cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab252438 at 1/100 dilution (4.69 μg/ml), ab255609 at 1/1000 dilution (0.615 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP4 antibody [EPR3579] (AB92501)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FABP4 with Purified ab92501 at 1/16,000 dilution (0.03 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-FABP4 antibody [EPR3579] (AB92501)

Fluorescence multiplex immunohistochemical analysis of the Human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-CaSR (ab259846, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B : anti-CaSR stained on parathyroid chief cells. Panel C : anti-Cytochrome C stained on parathyroid oxyphil cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab259846 at 1/5000 dilution (0.103 μg/ml), ab247438 at 1/5000 dilution (0.195 μg/ml), and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-FABP4 antibody [EPR3579] (AB92501)

Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B : anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C : anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then ab247438 at 1/5000 dilution (0.195 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (AB92501)

Immunocytochemistry/ Immunofluorescence analysis of 3T3-L1 (Mouse embryonic fibroblast) differentiated for 6 days cells labeling FABP4 with Purifiedab92501 at 1/50 dilution (9.9 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 μg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Lab

Western blot - Anti-FABP4 antibody [EPR3579] (AB92501)

FABP4 is abundantly expressed in adipose tissue and at a lower level in lung, heart, skin, kidney, liver and brain (PMID : 23143994).

All lanes:

Western blot - Anti-FABP4 antibody [EPR3579] (ab92501) at 1/1000 dilution

Lane 1:

Mouse brown adipose tissue lysates at 20 µg

Lane 2:

Mouse heart lysates at 20 µg

Lane 3:

Mouse kidney lysates at 20 µg

Lane 4:

Mouse lung lysates at 20 µg

Lane 5:

Human adipose tissue lysates at 20 µg

Lane 6:

Rat adipose tissue lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-FABP4 antibody [EPR3579] (AB92501)

FABP4 (green) was detected using FABP4 primary antibody (unpurified ab92501; diluted 1/1000). Alpha tubulin (red) was detected using our mouse monoclonal (ab7291) antibody. Cells were imaged by confocal microscopy, using z-stack for adipocyte-like cells.