Rabbit Polyclonal FABP5 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Fabp5.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.31% Thimerosal (merthiolate), 0.06% Sodium azide
Constituents: 30.75% BSA, 5.54% Sodium chloride, 1.23% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Fabp5. The exact immunogen used to generate this antibody is proprietary information. Database link Q05816
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Tested |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 0.10000-0.50000 µg/mL | Notes - |
Species Mouse | Dilution info 0.1-0.5 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 2-5 µg/mL | Notes - |
Species Rat | Dilution info 2-5 µg/mL | Notes - |
Associated Products
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Target data
Function
Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (By similarity). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity).
Alternative names
Fatty acid-binding protein 5, Cutaneous fatty acid-binding protein, DA11, Epidermal-type fatty acid-binding protein, C-FABP, E-FABP, Fabp5
Recommended products
Rabbit Polyclonal FABP5 antibody. Suitable for WB, IHC-P and reacts with Rat, Mouse samples. Immunogen corresponding to Synthetic Peptide within Mouse Fabp5.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.31% Thimerosal (merthiolate), 0.06% Sodium azide
Constituents: 30.75% BSA, 5.54% Sodium chloride, 1.23% Disodium hydrogenorthophosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Fabp5. The exact immunogen used to generate this antibody is proprietary information. Database link Q05816
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FABP5 protein also known as Fatty Acid Binding Protein 5 or Epidermal-type Fatty Acid Binding Protein (E-FABP) serves an important role in the transport and metabolism of long-chain fatty acids. It has a molecular mass of approximately 15 kDa. FABP5 is expressed in multiple tissues with notably high expression in the epidermis brain liver and adipose tissue. This protein assists in intracellular fatty acid movement affecting cellular lipid catabolism.
- Biological function summary
FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.
- Pathways
FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.
- Associated diseases and disorders
Researchers have linked FABP5 to conditions like psoriasis and type 2 diabetes. In psoriasis the overexpression of FABP5 has been observed in skin lesions suggesting that it might contribute to the pathological process. FABP5 is also connected to metabolic syndrome and insulin resistance as its dysregulation can influence lipid and glucose metabolism processes. In these disorders FABP5 interacts with proteins like PPARγ indicating possible intervention points for therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-FABP5 antibody (ab151772)
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with ab151772 at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1/5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP5 at approximately 15 kDa. The expected band size for FABP5 is at 15 kDa.All lanes: Western blot - Anti-FABP5 antibody (ab151772) at 0.5 µg/mL
Lane 1: rat lung tissue lysates at 30 µg
Lane 2: rat intestine tissue lysates at 30 µg
Lane 3: mouse lung tissue lysates at 30 µg
SecondaryAll lanes: goat anti-rabbit IgG-HRP at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab151772)
FABP5 was detected in a paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab151772 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (ab151772)
FABP5 was detected in a paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml ab151772 overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
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