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AB37267

Anti-FABP5 antibody

5

(1 Review)

|

(9 Publications)

Rabbit Polyclonal FABP5 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human FABP5.

View Alternative Names

Fatty acid-binding protein 5, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog, E-FABP, PA-FABP, FABP5

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (AB37267)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody (AB37267)

ab37267 (1μg/mL) staining FABP5 in human oesophagus using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm and nuclei in stratified squamous epithelium cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human FABP5.

Q01469

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Immunoaffinity chromatography on a column with immobilized recombinant human FABP5.
Storage buffer
pH: 7.2 Constituents: PBS, 0.58% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The FABP5 protein also known as Fatty Acid Binding Protein 5 or Epidermal-type Fatty Acid Binding Protein (E-FABP) serves an important role in the transport and metabolism of long-chain fatty acids. It has a molecular mass of approximately 15 kDa. FABP5 is expressed in multiple tissues with notably high expression in the epidermis brain liver and adipose tissue. This protein assists in intracellular fatty acid movement affecting cellular lipid catabolism.
Biological function summary

FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.

Pathways

FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.

Researchers have linked FABP5 to conditions like psoriasis and type 2 diabetes. In psoriasis the overexpression of FABP5 has been observed in skin lesions suggesting that it might contribute to the pathological process. FABP5 is also connected to metabolic syndrome and insulin resistance as its dysregulation can influence lipid and glucose metabolism processes. In these disorders FABP5 interacts with proteins like PPARγ indicating possible intervention points for therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed : 21395585, PubMed : 22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity). May be involved in keratinocyte differentiation (PubMed : 8092987).
See full target information FABP5
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:15390 PubMed38965292

2024

Concomitant decrease of E- and A-FABP expression predicts worse survival in urothelial bladder cancer patients.

Applications

Unspecified application

Species

Unspecified reactive species

Inès Saizonou,Isabelle Lascombe,Franck Monnien,Isabelle Bedgedjian,François Kleinclauss,Marie-Paule Algros,Sylvie Fauconnet

Journal of gastric cancer 23:340-354 PubMed37129157

2023

Fatty Acid Binding Protein 5 (FABP5) Promotes Aggressiveness of Gastric Cancer Through Modulation of Tumor Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Mei-Qing Qiu,Hui-Jun Wang,Ya-Fei Ju,Li Sun,Zhen Liu,Tao Wang,Shi-Feng Kan,Zhen Yang,Ya-Yun Cui,You-Qiang Ke,Hong-Min He,Shu Zhang

Journal of cellular and molecular medicine 25:9199-9213 PubMed34431227

2021

CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Yaser Alduais,Kai Zhang,Xiaoli Zhu,Baoan Chen

Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 27:940-947 PubMed33891336

2021

Identification of skin aging biomarkers correlated with the biomechanical properties.

Applications

Unspecified application

Species

Unspecified reactive species

Seo Hyeong Kim,Ji Hye Kim,Jang Mi Suk,Young In Lee,Jihee Kim,Ju Hee Lee,Kwang Hoon Lee

BMC medical genomics 11:73 PubMed30157864

2018

Differentially expressed proteins in positive versus negative HNSCC lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandra Vidotto,Giovana M Polachini,Marina de Paula-Silva,Sonia M Oliani,Tiago Henrique,Rossana V M López,Patrícia M Cury,Fabio D Nunes,José F Góis-Filho,Marcos B de Carvalho,Andréia M Leopoldino,Eloiza H Tajara

Oncology letters 14:4772-4778 PubMed29085478

2017

Effect of FABP5 gene silencing on the proliferation, apoptosis and invasion of human gastric SGC-7901 cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guanjie Zhao,Minfei Wu,Xiaofeng Wang,Zhenwu Du,Guizhen Zhang

PloS one 12:e0173035 PubMed28253328

2017

Reprogramming of the retinoic acid pathway in decidualizing human endometrial stromal cells.

Applications

WB

Species

Unspecified reactive species

Rie Ozaki,Keiji Kuroda,Yuko Ikemoto,Asako Ochiai,Akemi Matsumoto,Jun Kumakiri,Mari Kitade,Atsuo Itakura,Joanne Muter,Jan J Brosens,Satoru Takeda

International journal of cancer 138:1947-58 PubMed26594836

2015

Abscisic-acid-induced cellular apoptosis and differentiation in glioma via the retinoid acid signaling pathway.

Applications

WB

Species

Unspecified reactive species

Nan Zhou,Yu Yao,Hongxing Ye,Wei Zhu,Liang Chen,Ying Mao

Arteriosclerosis, thrombosis, and vascular biology 29:767-73 PubMed19286631

2009

TIP47, a lipid cargo protein involved in macrophage triglyceride metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Insa Buers,Horst Robenek,Stefan Lorkowski,Yvonne Nitschke,Nicholas J Severs,Oliver Hofnagel
View all publications
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Product promise

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