Rabbit Polyclonal FABP5 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 9 publications.
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- Cell death & disease 14:1252023NSUN2 promotes osteosarcoma progression by enhancing the stability of FABP5 mRNA via mC methylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Yang,Renxiong Wei,Sheng Zhang,Sang Hu,Xiaoxiao Liang,Zhiqiang Yang,Chong Zhang,Yufeng Zhang,Lin Cai,Yuanlong XiePubMed 36792587
- Cancer biology & therapy 23:424-4382022Fatty acid binding protein 5 promotes the proliferation, migration, and invasion of hepatocellular carcinoma cells by degradation of Krüppel-like factor 9 mediated by miR-889-5p via cAMP-response element binding protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanping Tang,Kezhi Li,Bangli Hu,Zhengmin Cai,Jilin Li,Hao Tao,Ji CaoPubMed 35816613
- Biochemical genetics 59:1648-16652021CircARL8B Contributes to the Development of Breast Cancer Via Regulating miR-653-5p/HMGA2 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHansheng Wu,Jingyun Xu,Guoliang Gong,Yuanxin Zhang,Shenggui WuPubMed 34050452
- Journal of cellular and molecular medicine 25:4487-45002021FABP5 enhances malignancies of lower-grade gliomas via canonical activation of NF-κB signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYichang Wang,Alafate Wahafu,Wei Wu,Jianyang Xiang,Longwei Huo,Xudong Ma,Ning Wang,Hao Liu,Xiaobin Bai,Dongze Xu,Wanfu Xie,Maode Wang,Jia WangPubMed 33837625
- Nature communications 11:27682020Cellular census of human fibrosis defines functionally distinct stromal cell types and states.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThomas B Layton et. al.PubMed 32488016
- Cancer management and research 10:6275-62842018Altered epidermal fatty acid-binding protein expression in hepatocellular carcinoma predicts unfavorable outcomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJia-Bin Lu et. al.PubMed 30538573
- Proteome science 16:142018Comparative iTRAQ proteomics revealed proteins associated with horn development in yak.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingna Li,Xiaoyun Wu,Xian Guo,Pengjia Bao,Xuezhi Ding,Min Chu,Chunnian Liang,Ping YanPubMed 30061793
- International journal of oncology 52:787-8032018Tumorigenic proteins upregulated in the MYCN-amplified IMR-32 human neuroblastoma cells promote proliferation and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHayat Zaatiti et. al.PubMed 29328367
- PloS one 10:e01270032015Level of Fatty Acid Binding Protein 5 (FABP5) Is Increased in Sputum of Allergic Asthmatics and Links to Airway Remodeling and Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHille Suojalehto et. al.PubMed 26020772
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Cow, Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed:21395585, PubMed:22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity). May be involved in keratinocyte differentiation (PubMed:8092987).
Alternative names
Fatty acid-binding protein 5, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog, E-FABP, PA-FABP, FABP5
Recommended products
Rabbit Polyclonal FABP5 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 9 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FABP5 protein also known as Fatty Acid Binding Protein 5 or Epidermal-type Fatty Acid Binding Protein (E-FABP) serves an important role in the transport and metabolism of long-chain fatty acids. It has a molecular mass of approximately 15 kDa. FABP5 is expressed in multiple tissues with notably high expression in the epidermis brain liver and adipose tissue. This protein assists in intracellular fatty acid movement affecting cellular lipid catabolism.
- Biological function summary
FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.
- Pathways
FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.
- Associated diseases and disorders
Researchers have linked FABP5 to conditions like psoriasis and type 2 diabetes. In psoriasis the overexpression of FABP5 has been observed in skin lesions suggesting that it might contribute to the pathological process. FABP5 is also connected to metabolic syndrome and insulin resistance as its dysregulation can influence lipid and glucose metabolism processes. In these disorders FABP5 interacts with proteins like PPARγ indicating possible intervention points for therapeutic targeting.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-FABP5 antibody (ab84028)
Lanes 1-3: Merged signal (red and green). Green - ab84028 observed at 17 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab84028 Anti-FABP5 antibody was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FABP5 knockout HeLa cell line ab265905 (knockout cell lysate Human FABP5 knockout HeLa cell lysate ab257431) was used. Wild-type and FABP5 knockout samples were subjected to SDS-PAGE. ab84028 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-FABP5 antibody (ab84028) at 1/500 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FABP5 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FABP5 knockout HeLa cell line (Human FABP5 knockout HeLa cell line ab265905)
Lane 3: PC-3 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 15 kDa
Observed band size: 17 kDa
Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody (ab84028)
ICC/IF image of ab84028 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84028, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 5µg/ml, and in 100% Methanol fixed (5 min) HeLa, Hek293, HepG2, MCF-7 cells at 5µg/ml.
Immunoprecipitation - Anti-FABP5 antibody (ab84028)
FABP5 was immunoprecipitated using 0.5mg A431 whole cell extract, 5μg of Rabbit polyclonal to and 50μl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40μl SDS loading buffer and incubated for 10min at 70°C; 10μl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab84028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (Mouse monoclonal [SB62a] Anti-Rabbit IgG light chain (HRP) ab99697).
Band: 16kDa; FABP5.
All lanes: Immunoprecipitation - Anti-FABP5 antibody (ab84028)
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Exposure time: 20min
Western blot - Anti-FABP5 antibody (ab84028)
All lanes: Western blot - Anti-FABP5 antibody (ab84028) at 1 µg/mL
Lane 1: Human skin tissue lysate - total protein (ab30166) at 10 µg
Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 16 kDa
Exposure time: 3min
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