Rabbit Recombinant Monoclonal FABP5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples.
Images
![Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--western-blot-img425731.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)")
![Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunocytochemistry-immunofluorescence-img426026.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425732.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425734.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425733.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ChIP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ChIP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Intracellular carrier for long-chain fatty acids and related active lipids, such as endocannabinoids, that regulate the metabolism and actions of the ligands they bind. In addition to the cytosolic transport, selectively delivers specific fatty acids from the cytosol to the nucleus, wherein they activate nuclear receptors (PubMed:21395585, PubMed:22170058). Delivers retinoic acid to the nuclear receptor peroxisome proliferator-activated receptor delta; which promotes proliferation and survival. May also serve as a synaptic carrier of endocannabinoid at central synapses and thus controls retrograde endocannabinoid signaling. Modulates inflammation by regulating PTGES induction via NF-kappa-B activation, and prostaglandin E2 (PGE2) biosynthesis during inflammation (By similarity). May be involved in keratinocyte differentiation (PubMed:8092987).
Alternative names
Fatty acid-binding protein 5, Epidermal-type fatty acid-binding protein, Psoriasis-associated fatty acid-binding protein homolog, E-FABP, PA-FABP, FABP5
Recommended products
Rabbit Recombinant Monoclonal FABP5 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22552-64
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255291 is the carrier-free version of Anti-FABP5 antibody [EPR22552-64] ab255276.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The FABP5 protein also known as Fatty Acid Binding Protein 5 or Epidermal-type Fatty Acid Binding Protein (E-FABP) serves an important role in the transport and metabolism of long-chain fatty acids. It has a molecular mass of approximately 15 kDa. FABP5 is expressed in multiple tissues with notably high expression in the epidermis brain liver and adipose tissue. This protein assists in intracellular fatty acid movement affecting cellular lipid catabolism.
- Biological function summary
FABP5 interacts with specific ligands such as long-chain fatty acids which influences cellular processes like growth differentiation and energy homeostasis. It is not known to be part of a larger complex. This protein is integral in regulating lipid signaling pathways and responses due to its binding capacity which modulates the availability of fatty acids for metabolic processes. Its expression pattern hints at a possible role in the body's adaptation to dietary lipid intake.
- Pathways
FABP5 holds a significant position in the peroxisome proliferator-activated receptor (PPAR) signaling pathway and the fatty acid metabolism pathway. In the PPAR signaling pathway FABP5 influences the activity of PPARβ/δ receptors which regulate gene expression involved in lipid metabolism and energy homeostasis. It is related to other fatty acid binding proteins such as FABP4 which similarly participate in lipid metabolic processes and have roles in the regulation of lipid and glucose metabolism.
- Associated diseases and disorders
Researchers have linked FABP5 to conditions like psoriasis and type 2 diabetes. In psoriasis the overexpression of FABP5 has been observed in skin lesions suggesting that it might contribute to the pathological process. FABP5 is also connected to metabolic syndrome and insulin resistance as its dysregulation can influence lipid and glucose metabolism processes. In these disorders FABP5 interacts with proteins like PPARγ indicating possible intervention points for therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--western-blot-img425731.jpg "Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
This data was developed using the same antibody clone in a different buffer formulation (Anti-FABP5 antibody [EPR22552-64] ab255276).
Blocking and dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 25260874).
Negative control: T47D (PMID: 21356353).All lanes: Western blot - Anti-FABP5 antibody [EPR22552-64] (Anti-FABP5 antibody [EPR22552-64] ab255276) at 1/1000 dilution
Lane 1: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3: PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate at 20 µg
Lane 4: T-47D (human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 15 kDa
Exposure time: 8s
![Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunocytochemistry-immunofluorescence-img426026.jpg "Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 was used as a counterstain (red).
The nuclear counterstain is DAPI (blue).
Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells.
Negative Control: T-47D (PMID: 21356353).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP5 antibody [EPR22552-64] ab255276).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425732.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
FABP5 immunohistochemistry staining of human skin using rabbit anti-FABP5 antibody
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID: 8092987) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP5 antibody [EPR22552-64] ab255276).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425734.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID: 24114376) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP5 antibody [EPR22552-64] ab255276).![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img425733.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID: 19625659) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-FABP5 antibody [EPR22552-64] ab255276).![Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--western-blot-img425730.jpg "Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)
This data was developed using the same antibody clone in a different buffer formulation (Anti-FABP5 antibody [EPR22552-64] ab255276).
Lanes 1-3: Merged signal (red and green). Green - Anti-FABP5 antibody [EPR22552-64] ab255276 observed at 17 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
Anti-FABP5 antibody [EPR22552-64] ab255276 Anti-FABP5 antibody [EPR22552-64] was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human FABP5 knockout HeLa cell line ab265905 (knockout cell lysate Human FABP5 knockout HeLa cell lysate ab257431) was used. Wild-type and FABP5 knockout samples were subjected to SDS-PAGE. Anti-FABP5 antibody [EPR22552-64] ab255276 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-FABP5 antibody [EPR22552-64] (Anti-FABP5 antibody [EPR22552-64] ab255276) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: FABP5 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human FABP5 knockout HeLa cell lysate (Human FABP5 knockout HeLa cell lysate ab257431)
Lane 2: Western blot - Human FABP5 knockout HeLa cell line (Human FABP5 knockout HeLa cell line ab265905)
Lane 3: PC-3 cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Observed band size: 17 kDa
![Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291), expandable thumbnail](https://content.abcam.com/products/images/fabp5-antibody-epr22552-64-bsa-and-azide-free-ab255291--western-blot-img425730.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (ab255291)")
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