Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID : 24114376) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255276).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

Immunohistochemical analysis of paraffin-embedded human skin tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human skin (PMID : 8092987) is observed. Counter stained with hematoxylin. Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255276).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID : 19625659) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255276).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 (human breast adenocarcinoma epithelial cell) labeling FABP5 with ab255276 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 was used as a counterstain (red).

The nuclear counterstain is DAPI (blue).

Confocal image showing cytoplasmic and nuclear staining in MDA-MB-231 cells.

Negative Control : T-47D (PMID : 21356353).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab255276).

• WB

Supplier Data

Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

This data was developed using the same antibody clone in a different buffer formulation (ab255276).

Lanes 1-3 : Merged signal (red and green). Green - ab255276 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.

ab255276 Anti-FABP5 antibody [EPR22552-64] was shown to specifically react with FABP5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265905 (knockout cell lysate ab257431) was used.  Wild-type and FABP5 knockout samples were subjected to SDS-PAGE.  ab255276 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively.  Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-FABP5 antibody [EPR22552-64] (<a href='/en-us/products/primary-antibodies/fabp5-antibody-epr22552-64-ab255276'>ab255276</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

FABP5 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human FABP5 knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-fabp5-knockout-hela-cell-lysate-ab257431'>ab257431</a>)

Lane 2:

Western blot - Human FABP5 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-fabp5-knockout-hela-cell-line-ab265905'>ab265905</a>)

Lane 3:

PC-3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Observed band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-FABP5 antibody [EPR22552-64] - BSA and Azide free (AB255291)

This data was developed using the same antibody clone in a different buffer formulation (ab255276).

Blocking and dilution buffer : 5% NFDM/TBST.The molecular weight observed is consistent with what has been described in the literature (PMID : 25260874).Negative control : T47D (PMID : 21356353).

All lanes:

Western blot - Anti-FABP5 antibody [EPR22552-64] (<a href='/en-us/products/primary-antibodies/fabp5-antibody-epr22552-64-ab255276'>ab255276</a>) at 1/1000 dilution

Lane 1:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 3:

PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

Lane 4:

T-47D (human ductal breast epithelial tumor cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 15 kDa

false

Exposure time: 8s