AB227256

Anti-FACL4 antibody

Name: Anti-FACL4 antibody (ab227256) | Abcam
Brand: Abcam Limited
SKU: AB227256
Price: 525 USD
Availability: InStock

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(8 Publications)

Rabbit Polyclonal FACL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL4.

View Alternative Names

ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4

8 Images

Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (AB227256)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (AB227256)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FACL4 (green) using ab227256 at 1/200 dilution in ICC/IF. Nuclear Counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (AB227256)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (AB227256)

Paraffin-embedded mouse kidney tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (AB227256)

Paraffin-embedded rat duodenum tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-FACL4 antibody (AB227256)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

Lane 2:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-FACL4 antibody (AB227256)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-FACL4 antibody (AB227256)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution

Lane 1:

Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

FACL4 shRNA-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Predicted band size: 134 kDa,79 kDa

Observed band size: 200 kDa

false

Supplier Data

Western blot - Anti-FACL4 antibody (AB227256)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-FACL4 antibody (AB227256)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution

All lanes:

RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human ACSL4. The exact immunogen used to generate this antibody is proprietary information.

O60488

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

FACL4 also known as ACSL4 is a member of the long-chain acyl-CoA synthetase (ACSL) family. This enzyme catalyzes the activation of long-chain fatty acids by forming a thioester bond with coenzyme A. FACL4 plays a critical role in lipid metabolism. The molecular weight of FACL4 is approximately 80 kDa. This protein is expressed in various tissues with a higher presence in the brain and testis reflecting its important function in lipid-related processes in these areas.

Biological function summary

FACL4 contributes significantly to lipid metabolism and is involved in the synthesis of complex lipids. It facilitates the integration of long-chain fatty acids into phospholipids and triglycerides. FACL4 often functions as part of larger lipid synthesis and modification complexes working closely with other enzymes to maintain membrane integrity and signal transduction. Its activity influences cellular responses to changes in lipid availability and metabolic demands.

Pathways

FACL4 functions within the arachidonic acid metabolism and glycerophospholipid metabolic pathways. In these pathways other proteins such as COX and LOX interact with FACL4 to regulate inflammatory responses and membrane lipid composition. The enzyme’s role in these pathways highlights its contribution to managing the balance between pro-inflammatory and anti-inflammatory metabolites which are essential for cell signaling and homeostasis.

FACL4 is implicated in several neuropsychiatric conditions including X-linked adrenoleukodystrophy and breast cancer. In the context of these diseases FACL4 interacts with proteins like ALDP in adrenoleukodystrophy. Disrupted FACL4 activity can lead to abnormal lipid accumulation or impaired lipid signaling underlying the pathophysiology of these conditions. Understanding the role of FACL4 in these disorders highlights its potential as a therapeutic target for correcting lipid metabolism dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed : 21242590, PubMed : 22633490, PubMed : 24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed : 21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed : 21242590).

See full target information ACSL4

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Hereditas 162:135 PubMed40682199

2025

RBM15 promotes hypoxia/reoxygenation-induced ferroptosis in human cardiomyocytes by mediating m6A modification of ACSL4.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Cheng,Jiamin Wan,Yingyue Xu,Shasha Liu,Linfeng Li,Jing Zhou,Fuyan Xie

Cell communication and signaling : CCS 22:464 PubMed39350150

2024

ER-mitochondria contact sites regulate hepatic lipogenesis via Ip3r-Grp75-Vdac complex recruiting Seipin.

Applications

Unspecified application

Species

Unspecified reactive species

Ying-Jia Chi,Zhen-Yu Bai,Guang-Li Feng,Xiao-Hong Lai,Yu-Feng Song

Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 35:176-190 PubMed37180834

2023

Aqueous-soluble components of sporoderm-removed spore powder promote ferroptosis in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangping Wu,Qingnan Wu,Yan Wang,Yehai Liu,Zhenhao Li,Qingchuan Liu,Zhengming Huang,Mingyan Li,Bin Zhang,Qimin Zhan

Nature communications 14:1660 PubMed36966149

2023

Continuous Spatiotemporal Therapy of A Full-API Nanodrug via Multi-Step Tandem Endogenous Biosynthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Fang,Sa Wang,Yueyue Song,Meng Sun,Wen-Cheng Chen,Dongxu Zhao,Jinfeng Zhang

Frontiers in oncology 12:929838 PubMed36059676

2022

ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhu,Zhuoyu Xiao,Haoyu Yuan,Hu Tian,Taoyi Chen,Qi Chen,Mingkun Chen,Jiankun Yang,Qizhao Zhou,Wenbin Guo,Kangyi Xue,Ming Xia,Jiming Bao,Cheng Yang,Haifeng Duan,Hongyi Wang,Zhipeng Huang,Cundong Liu,Junhao Zhou

Oxidative medicine and cellular longevity 2022:7671324 PubMed35936219

2022

miR-494-3p Promotes Erastin-Induced Ferroptosis by Targeting REST to Activate the Interplay between SP1 and ACSL4 in Parkinson's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jianjun Ma,Xiaohuan Li,Yongyan Fan,Dawei Yang,Qi Gu,Dongsheng Li,Siyuan Chen,Shaopu Wu,Jinhua Zheng,Hongqi Yang,Xue Li

Bulletin of experimental biology and medicine 172:423-429 PubMed35175488

2022

Anti-Inflammatory and Anti-Oxidant Activity of Ultra-Short Wave Diathermy on LPS-Induced Rat Lung Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Q Wu,M Qu,P Zhong,Y Zeng,J Wang,Q Zhang,Ting Wang,D Liu,L Yang,J Zhou,Tong Wang

European journal of histochemistry : EJH 65: PubMed34587716

2021

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi Ye

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FACL4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (AB227256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (AB227256)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (AB227256)

Western blot - Anti-FACL4 antibody (AB227256)

Western blot - Anti-FACL4 antibody (AB227256)

Western blot - Anti-FACL4 antibody (AB227256)

Western blot - Anti-FACL4 antibody (AB227256)

Western blot - Anti-FACL4 antibody (AB227256)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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