Rabbit Polyclonal FACL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL4.
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- Chinese journal of cancer research = Chung-kuo yen cheng yen chiu 35:176-1902023Aqueous-soluble components of sporoderm-removed spore powder promote ferroptosis in oral squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangping Wu,Qingnan Wu,Yan Wang,Yehai Liu,Zhenhao Li,Qingchuan Liu,Zhengming Huang,Mingyan Li,Bin Zhang,Qimin ZhanPubMed 37180834
- Nature communications 14:16602023Continuous Spatiotemporal Therapy of A Full-API Nanodrug via Multi-Step Tandem Endogenous Biosynthesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFang Fang,Sa Wang,Yueyue Song,Meng Sun,Wen-Cheng Chen,Dongxu Zhao,Jinfeng ZhangPubMed 36966149
- Frontiers in oncology 12:9298382022ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhu,Zhuoyu Xiao,Haoyu Yuan,Hu Tian,Taoyi Chen,Qi Chen,Mingkun Chen,Jiankun Yang,Qizhao Zhou,Wenbin Guo,Kangyi Xue,Ming Xia,Jiming Bao,Cheng Yang,Haifeng Duan,Hongyi Wang,Zhipeng Huang,Cundong Liu,Junhao ZhouPubMed 36059676
- Oxidative medicine and cellular longevity 2022:76713242022miR-494-3p Promotes Erastin-Induced Ferroptosis by Targeting REST to Activate the Interplay between SP1 and ACSL4 in Parkinson's Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianjun Ma,Xiaohuan Li,Yongyan Fan,Dawei Yang,Qi Gu,Dongsheng Li,Siyuan Chen,Shaopu Wu,Jinhua Zheng,Hongqi Yang,Xue LiPubMed 35936219
- Bulletin of experimental biology and medicine 172:423-4292022Anti-Inflammatory and Anti-Oxidant Activity of Ultra-Short Wave Diathermy on LPS-Induced Rat Lung Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQ Wu,M Qu,P Zhong,Y Zeng,J Wang,Q Zhang,Ting Wang,D Liu,L Yang,J Zhou,Tong WangPubMed 35175488
- European journal of histochemistry : EJH 65:2021Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi YePubMed 34587716
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ACSL4. The exact immunogen used to generate this antibody is proprietary information. Database link O60488
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Tested | Expected |
| Rat | Tested | Tested | Expected |
| Pig | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig, Rhesus monkey | Dilution info - | Notes - |
Associated Products
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Target data
Function
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).
Alternative names
ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4
Recommended products
Rabbit Polyclonal FACL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ACSL4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ACSL4. The exact immunogen used to generate this antibody is proprietary information. Database link O60488
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
FACL4 also known as ACSL4 is a member of the long-chain acyl-CoA synthetase (ACSL) family. This enzyme catalyzes the activation of long-chain fatty acids by forming a thioester bond with coenzyme A. FACL4 plays a critical role in lipid metabolism. The molecular weight of FACL4 is approximately 80 kDa. This protein is expressed in various tissues with a higher presence in the brain and testis reflecting its important function in lipid-related processes in these areas.
- Biological function summary
FACL4 contributes significantly to lipid metabolism and is involved in the synthesis of complex lipids. It facilitates the integration of long-chain fatty acids into phospholipids and triglycerides. FACL4 often functions as part of larger lipid synthesis and modification complexes working closely with other enzymes to maintain membrane integrity and signal transduction. Its activity influences cellular responses to changes in lipid availability and metabolic demands.
- Pathways
FACL4 functions within the arachidonic acid metabolism and glycerophospholipid metabolic pathways. In these pathways other proteins such as COX and LOX interact with FACL4 to regulate inflammatory responses and membrane lipid composition. The enzyme’s role in these pathways highlights its contribution to managing the balance between pro-inflammatory and anti-inflammatory metabolites which are essential for cell signaling and homeostasis.
- Associated diseases and disorders
FACL4 is implicated in several neuropsychiatric conditions including X-linked adrenoleukodystrophy and breast cancer. In the context of these diseases FACL4 interacts with proteins like ALDP in adrenoleukodystrophy. Disrupted FACL4 activity can lead to abnormal lipid accumulation or impaired lipid signaling underlying the pathophysiology of these conditions. Understanding the role of FACL4 in these disorders highlights its potential as a therapeutic target for correcting lipid metabolism dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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8 product images
Western blot - Anti-FACL4 antibody (ab227256)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1: Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Lane 2: FACL4 shRNA-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg
Predicted band size: 134 kDa, 79 kDa
Observed band size: 200 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)
Paraffin-embedded mouse kidney tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody (ab227256)
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for FACL4 (green) using ab227256 at 1/200 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342 (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody (ab227256)
Paraffin-embedded rat duodenum tissue stained for FACL4 using ab227256 at 1/500 dilution in immunohistochemical analysis.
Western blot - Anti-FACL4 antibody (ab227256)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution
All lanes: RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa
Western blot - Anti-FACL4 antibody (ab227256)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract at 30 µg
Lane 2: Rat2 whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa
Western blot - Anti-FACL4 antibody (ab227256)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution
Lane 1: MCF7 (human breast adenocarcinoma cell line) whole cell extract at 30 µg
Lane 2: MDA-MB-231 (human breast adenocarcinoma cell line) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 79 kDa
Western blot - Anti-FACL4 antibody (ab227256)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-FACL4 antibody (ab227256) at 1/1000 dilution
All lanes: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg
Predicted band size: 79 kDa
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