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Rabbit Polyclonal FACL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Long-chain-fatty-acid--CoA ligase 4 aa 600 to C-terminus.


Images

Western blot - Anti-FACL4 antibody - Carboxyterminal end (AB137525), expandable thumbnail
  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (AB137525), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (AB137525), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (AB137525), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Long-chain-fatty-acid--CoA ligase 4 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60488

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Predicted
Predicted

Species

Rat, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Pig

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).

Alternative names

Recommended products

Rabbit Polyclonal FACL4 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human Long-chain-fatty-acid--CoA ligase 4 aa 600 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Long-chain-fatty-acid--CoA ligase 4 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60488
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FACL4 also known as ACSL4 is a member of the long-chain acyl-CoA synthetase (ACSL) family. This enzyme catalyzes the activation of long-chain fatty acids by forming a thioester bond with coenzyme A. FACL4 plays a critical role in lipid metabolism. The molecular weight of FACL4 is approximately 80 kDa. This protein is expressed in various tissues with a higher presence in the brain and testis reflecting its important function in lipid-related processes in these areas.

Biological function summary

FACL4 contributes significantly to lipid metabolism and is involved in the synthesis of complex lipids. It facilitates the integration of long-chain fatty acids into phospholipids and triglycerides. FACL4 often functions as part of larger lipid synthesis and modification complexes working closely with other enzymes to maintain membrane integrity and signal transduction. Its activity influences cellular responses to changes in lipid availability and metabolic demands.

Pathways

FACL4 functions within the arachidonic acid metabolism and glycerophospholipid metabolic pathways. In these pathways other proteins such as COX and LOX interact with FACL4 to regulate inflammatory responses and membrane lipid composition. The enzyme’s role in these pathways highlights its contribution to managing the balance between pro-inflammatory and anti-inflammatory metabolites which are essential for cell signaling and homeostasis.

Associated diseases and disorders

FACL4 is implicated in several neuropsychiatric conditions including X-linked adrenoleukodystrophy and breast cancer. In the context of these diseases FACL4 interacts with proteins like ALDP in adrenoleukodystrophy. Disrupted FACL4 activity can lead to abnormal lipid accumulation or impaired lipid signaling underlying the pathophysiology of these conditions. Understanding the role of FACL4 in these disorders highlights its potential as a therapeutic target for correcting lipid metabolism dysfunction.

Product promise

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4 product images

  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525), expandable thumbnail

    Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

    Lane 1: HeLa whole cell lysate at 30 µg

    Lane 2: NT2D1 whole cell lysate at 30 µg

    Predicted band size: 79 kDa

    Observed band size: 74 kDa

  • Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525), expandable thumbnail

    Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525)

    7.5% SDS PAGE

    All lanes: Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/5000 dilution

    Lane 1: NIH 3T3 whole cell lysate at 30 µg

    Lane 2: JC whole cell lysate at 30 µg

    Lane 3: BCL1 whole cell lysate at 30 µg

    Predicted band size: 79 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (ab137525)

    Immunohistochemical analysis of paraffin embedded HBL435 xenograft tissue labelling FACL4 with ab137525 at 1/500.

  • Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (ab137525)

    Immunofluorescence analysis of methanol fixed HeLa cells labelling FACL4 with ab137525 at 1/500 (Green). Alpha tubulin filaments were labelled using a suitable antibody(Red).

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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