Anti-FACL4 antibody - Carboxyterminal end

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling FACL4 protein at cytoplasm using ab137525 at a 1/500 dilution (Green). alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). DAPI was used for nuclear staining (Blue).

• IP

Supplier Data

Immunoprecipitation - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Immunoprecipitation of FACL4 protein from HeLa whole cell extracts using 5 µg of ab137525. Western blot analysis was performed using ab137525 diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/500 dilution

Lane 1:

HeLa whole cell extract (Input)

Lane 2:

HeLa whole cell extract with control IgG

Lane 3:

HeLa whole cell extract (+)

Secondary

All lanes:

HRP-conjugated anti rabbit IgG antibody

Predicted band size: 79 kDa

false

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Immunohistochemical analysis of paraffin-embedded rat stomach and kidney tissue stained for FACL4 protein with ab137525 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

Lane 1:

MCF-7 whole cell extract at 30 µg

Lane 2:

MDA-MB-231 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 79 kDa

false

• WB

Supplier Data

Western blot - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

Lane 1:

Neuro2a whole cell extract at 30 µg

Lane 2:

NIH-3T3 whole cell extract at 30 µg

Lane 3:

Raw264.7 whole cell extract at 30 µg

Lane 4:

C2C12 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 79 kDa

false

• WB

Supplier Data

Western blot - Anti-FACL4 antibody - Carboxyterminal end (AB137525)

Samples were separated by 7.5 % SDS.

All lanes:

Western blot - Anti-FACL4 antibody - Carboxyterminal end (ab137525) at 1/1000 dilution

Lane 1:

PC-12 whole cell extract at 30 µg

Lane 2:

Rat2 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 79 kDa

false