Name: Anti-FACL4 antibody [EPR8640]
SKU: ab155282
Rating: 4 (1 reviews)

Anti-FACL4 antibody [EPR8640] is a rabbit monoclonal antibody that is used in FACL4 western blot (WB), IHC, immunocytochemistry/immunofluorescence (ICC/IF), immunoprecipitation (IP), and flow cytometry. Suitable for human, mouse, and rat samples.

- Cited in over 140 publications
- Antibody clone EPR8640 is the most widely used clone for FACL4 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests

Related conjugates and formulations

ab203974
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Alexa Fluor® 647 Anti-FACL4 antibody [EPR8640]
ab240135
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Anti-FACL4 antibody [EPR8640] - BSA and Azide free
ab305645
Conjugated
APC Anti-FACL4 antibody [EPR8640]
ab305646
Conjugated
HRP Anti-FACL4 antibody [EPR8640]
ab308801
Conjugated
AP Anti-FACL4 antibody [EPR8640]
ab312539
Conjugated
Alexa Fluor® 568 Anti-FACL4 antibody [EPR8640]
ab312062
Conjugated
Alexa Fluor® 555 Anti-FACL4 antibody [EPR8640]
ab204380
Conjugated
Alexa Fluor® 488 Anti-FACL4 antibody [EPR8640]
ab305644
Conjugated
PE Anti-FACL4 antibody [EPR8640]

Images

Western blot - Anti-FACL4 antibody [EPR8640] (AB155282), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Expected
Rat	Expected	Tested	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes -
Species Rat	Dilution info 1/10000 - 1/50000	Notes -
Species Human	Dilution info 1/10000 - 1/50000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/10 - 1/100	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/250	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

See full target information ACSL4

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation (PubMed:21242590, PubMed:22633490, PubMed:24269233). Preferentially activates arachidonate and eicosapentaenoate as substrates (PubMed:21242590). Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion (PubMed:21242590).

Alternative names

ACS4, FACL4, LACS4, ACSL4, Long-chain-fatty-acid--CoA ligase 4, Arachidonate--CoA ligase, Long-chain acyl-CoA synthetase 4, LACS 4

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR8640
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Product Specifications

Anti-FACL4 antibody [EPR8640] (ab155282) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in human, mouse, rat samples.
Anti-FACL4 antibody [EPR8640] (ab155282) specifically detects FACL4 (UniProt ID: O60488; Molecular weight: 79kDa) and is sold in 100 µL and 1 mL selling sizes.

Quality and Validation

Abcam's high quality manufacturing and validation processes ensure Anti-FACL4 antibody [EPR8640] (ab155282) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-FACL4 antibody [EPR8640] (ab155282) has been cited over 144 times in peer reviewed journals and is trusted by the scientific community.

Related Products
Conjugation-ready, carrier free format available for antibody clone EPR8640 - ab240135.
Antibody clone EPR8640 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 555, Alexa Fluor® 568 (ab203974, ab204380, ab305644, ab305645, ab305646, ab308801, ab312062, ab312539).

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

FACL4 also known as ACSL4 is a member of the long-chain acyl-CoA synthetase (ACSL) family. This enzyme catalyzes the activation of long-chain fatty acids by forming a thioester bond with coenzyme A. FACL4 plays a critical role in lipid metabolism. The molecular weight of FACL4 is approximately 80 kDa. This protein is expressed in various tissues with a higher presence in the brain and testis reflecting its important function in lipid-related processes in these areas.

Biological function summary

FACL4 contributes significantly to lipid metabolism and is involved in the synthesis of complex lipids. It facilitates the integration of long-chain fatty acids into phospholipids and triglycerides. FACL4 often functions as part of larger lipid synthesis and modification complexes working closely with other enzymes to maintain membrane integrity and signal transduction. Its activity influences cellular responses to changes in lipid availability and metabolic demands.

Pathways

FACL4 functions within the arachidonic acid metabolism and glycerophospholipid metabolic pathways. In these pathways other proteins such as COX and LOX interact with FACL4 to regulate inflammatory responses and membrane lipid composition. The enzyme’s role in these pathways highlights its contribution to managing the balance between pro-inflammatory and anti-inflammatory metabolites which are essential for cell signaling and homeostasis.

Associated diseases and disorders

FACL4 is implicated in several neuropsychiatric conditions including X-linked adrenoleukodystrophy and breast cancer. In the context of these diseases FACL4 interacts with proteins like ALDP in adrenoleukodystrophy. Disrupted FACL4 activity can lead to abnormal lipid accumulation or impaired lipid signaling underlying the pathophysiology of these conditions. Understanding the role of FACL4 in these disorders highlights its potential as a therapeutic target for correcting lipid metabolism dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

11 product images

Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution
Lane 1: Mouse brain tissue lysate at 20 µg
Lane 2: Rat brain tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-FACL4 antibody [EPR8640] (ab155282) at 1/50000 dilution
All lanes: HepG2 whole cell lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution
Lane 1: HeLa whole cell lysate at 20 µg
Lane 2: HEK293 whole cell lysate at 20 µg
Lane 3: Human fetal kidney tissue lysate at 20 µg
Secondary
All lanes: Peroxidase-conjugate goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 79 kDa
Observed band size: 79 kDa
Immunoprecipitation - Anti-FACL4 antibody [EPR8640] (ab155282)
ab155282 (purified) at 1/30 immunoprecipitating FACL4 in HepG2 whole cell lysate.
Lane 1 (input): HepG2 whole cell lysate (10μg)
Lane 2 (+): ab155282 + HepG2 whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab155282 in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
All lanes: Immunoprecipitation - Anti-FACL4 antibody [EPR8640] (ab155282)
Predicted band size: 79 kDa
Observed band size: 79 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody [EPR8640] (ab155282)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling FACL4 with purified ab155282 at 1/200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-FACL4 antibody [EPR8640] (ab155282)
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling FACL4 with purified ab155282 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/200) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry (Intracellular) - Anti-FACL4 antibody [EPR8640] (ab155282)
Intracellular Flow Cytometry analysis of 293T cells labelling FACL4 with purified ab155282 at 1/100 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)
All lanes: Western blot - Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution
Lane 1: HepG2 lysate at 10 µg
Lane 2: HeLa lysate at 10 µg
Lane 3: 293T lysate at 10 µg
Lane 4: Fetal kidney lysate, human at 10 µg
Predicted band size: 79 kDa
Western blot - Anti-FACL4 antibody [EPR8640] (ab155282)
All lanes: Western blot - Anti-FACL4 antibody [EPR8640] (ab155282) at 1/10000 dilution
Lane 1: Rat brain tissue lysate
Lane 2: Rat heart tissue lysate
Lane 3: Rat kidney tissue lysate
Lane 4: Rat spleen tissue lysate
Predicted band size: 79 kDa
Observed band size: 75 kDa
Exposure time: 1min
Flow Cytometry (Intracellular) - Anti-FACL4 antibody [EPR8640] (ab155282)
Intracellular flow cytometric analysis of permeabilized 293T cells labelling FACL4with unpurified ab155282 at a dilution of 1/10 (red) compared to a rabbit IgG (negative) (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FACL4 antibody [EPR8640] (ab155282)
Immunohistochemical analysis of formalin fixed paraffin embedded Human liver carcinoma labelling FACL4 with ab155282 at a concentration of 0.5 µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.
ab155282 anti FACL4 antibody EPR8640 was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)