Anti-Factor B antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB192577)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Factor B with ab192577 at 1/50 dilution.

• WB

Supplier Data

Western blot - Anti-Factor B antibody (AB192577)

All lanes:

Western blot - Anti-Factor B antibody (ab192577) at 1/500 dilution

Lane 1:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 3:

U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate

Lane 4:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Predicted band size: 85 kDa

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• WB

CiteAb

Western blot - Anti-Factor B antibody (AB192577)

Factor B western blot using anti-Factor B antibody ab192577. Publication image and figure legend from Sun, X., Ni, Y., et al., 2022, Cell Death Dis, PubMed 36494334.

ab192577 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab192577 please see the product overview.

Down regulation of CFB in liver protects against Con-A induced liver injury.a Western blot assay showing CFB expression in mouse livers after shRNA-CFB injection. b Representative co-immunofluorescent staining images for F4/80 with CFB (white arrows). Scale bar = 100 μm. c The strategy for establishing a mouse model of injection of shRNA-CFB and Con-A administration. d The ALT levels in serum of mice exposed to Con-A for 8 h, n = 6. e Representative HE-stained mouse livers. Scale bar = 100 μm. f Liver sections of were immunofluorescent stained with TUNEL. Scale bar = 200 μm. g Left, representative immunofluorescent staining images for ly6b. Scale bar = 100 μm. Right, quantitative determination of ly6b+ cells among groups as indicated, n = 4. h Left, representative immunofluorescent staining images for C3d. Scale bar = 100 μm. Right, quantitative determination of C3d area in a field of vision, n = 4. i Representative immunostaining images for C5b-9. Scale bar = 100 μm. j Left, representative immunofluorescent staining images for F4/80 (white arrows). Scale bar = 100 μm. Right, quantitative determination of F4/80+ cells among groups as indicated, n = 4. *p < 0.05. k Schematic working model on the role of mTORC1 activation in hepatocytes and KCs in the pathogenesis of ALD.

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