AB231072

Anti-Factor B antibody

Name: Anti-Factor B antibody (ab231072) | Abcam
Brand: Abcam Limited
SKU: AB231072

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(4 Publications)

Rabbit Polyclonal CFAB antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Bf, H2-Bf, Cfb, Complement factor B, C3/C5 convertase

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB231072)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB231072)

Formalin-fixed, paraffin-embedded rat liver tissue stained for Factor B using ab231072 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Factor B antibody (AB231072)

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Western blot - Anti-Factor B antibody (ab231072) at 1 µg/mL

All lanes:

Mouse serum

Predicted band size: 85 kDa

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Supplier Data

Western blot - Anti-Factor B antibody (AB231072)

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Western blot - Anti-Factor B antibody (ab231072) at 2 µg/mL

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Rat blood cell lysate

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-Factor B antibody (AB231072)

All lanes:

Western blot - Anti-Factor B antibody (ab231072) at 1 µg/mL

All lanes:

Rat serum

Predicted band size: 85 kDa

false

Supplier Data

Western blot - Anti-Factor B antibody (AB231072)

All lanes:

Western blot - Anti-Factor B antibody (ab231072) at 2 µg/mL

All lanes:

Recombinant rat Factor B protein

Predicted band size: 85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231072 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.

Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase.

See full target information Cfb

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 21:178 PubMed39034417

2024

PEDF-34 attenuates neurological deficit and suppresses astrocyte-dependent neuroinflammation by modulating astrocyte polarization via 67LR/JNK/STAT1 signaling pathway after subarachnoid hemorrhage in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wu,Yanchao Liu,Qiuguang He,Guangnan Ao,Ningbo Xu,Wangqing He,Xiao Liu,Lei Huang,Qian Yu,Hideki Kanamaru,Siyuan Dong,Shiyi Zhu,Ye Yuan,Mingyang Han,Yeping Ling,Lu Liu,Chenyu Wu,You Zhou,Prativa Sherchan,Jerry J Flores,Jiping Tang,Xionghui Chen,Xuying He,John H Zhang

Journal of animal science 101: PubMed37086180

2023

Preferential deposition of dairy derived fatty acids in muscle tissue is partially due to the upregulation of CD36 in a low-birth-weight swine model.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Wang,Yongbo She,Rabban Mangat,Alexander Makarowski,Bimol C Roy,Heather L Bruce,Michael K Dyck,Caroline Richard,Spencer D Proctor

Frontiers in immunology 12:649882 PubMed33868287

2021

Complement Initiation Varies by Sex in Intestinal Ischemia Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Miaomiao Wu,Jennifer M Rowe,Sherry D Fleming

Journal of cellular physiology 236:5012-5021 PubMed33400289

2021

Uromodulin aggravates renal tubulointerstitial injury through activation of the complement pathway in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Yu,Fei Pei,Qiaoling Sun,Fei Shen,Xiangdong Yang,Zhao Hu,Maojing Liu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Factor B antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Factor B antibody (AB231072)

Western blot - Anti-Factor B antibody (AB231072)

Western blot - Anti-Factor B antibody (AB231072)

Western blot - Anti-Factor B antibody (AB231072)

Western blot - Anti-Factor B antibody (AB231072)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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