AB72658

Anti-Factor B antibody

Name: Anti-Factor B antibody (ab72658) | Abcam
Brand: Abcam Limited
SKU: AB72658
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal CFAB antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human CFB aa 250-300.

View Alternative Names

BF, BFD, CFB, Complement factor B, C3/C5 convertase, Glycine-rich beta glycoprotein, PBF2, Properdin factor B, GBG

1 Images

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Western blot - Anti-Factor B antibody (AB72658)

All lanes:

Western blot - Anti-Factor B antibody (ab72658) at 1/200 dilution

Lane 1:

Extracts from K562 cells, treated with etoposide (25uM, 1hour) at 5 µg

Lane 2:

Extracts from K562 cells, treated with etoposide (25uM, 1hour) with Cl-peptide at 5 µg at 5 µg

Predicted band size: 85 kDa

Observed band size: 40 kDa,54 kDa,75 kDa,86 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human CFB aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P00751

Specificity

ab72658 detects a fragment from activated Factor B; the Bb fragment, resulting from cleavage adjacent to Lys260.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Factor B also known as B-factor is a protein that plays an important role in the immune system specifically in the complement pathway. It is sometimes referred to as factor B complement and its gene identifier is often referenced as KY21 NNL or factor B 1001. Factor B has a molecular mass of approximately 93 kDa. It is mainly expressed in the liver but can also be found in blood plasma in its inactive form as part of the innate immune response.

Biological function summary

Factor B is involved in the alternative complement pathway which serves as a part of the body's defense mechanism. It participates in the formation of the C3 convertase together with factor D and properdin initiating a cascade that can lead to the opsonization and elimination of pathogens. Factor B undergoes cleavage by factor D which generates the Ba and Bb fragments with Bb functioning as a serine protease within the C3 convertase complex.

Pathways

Factor B contributes to the complement system interacting notably in the alternative and lectin pathways. In these pathways Factor B associates with proteins like complement component C3. The alternative pathway is activated on microbial surfaces independent of antibodies. The involvement of Factor B in these pathways illustrates its role in pathogen recognition and further highlights its cooperation with complement proteins such as C3 and properdin.

Factor B has been associated with atypical hemolytic uremic syndrome (aHUS) a rare and serious disease affecting kidney function. Factor B involvement in aHUS links it to abnormal complement activation leading to a pathological condition. Additionally Factor B connects to age-related macular degeneration (AMD) a disorder where complement pathway genetic variations including those involving Factor B have shown associations. Understanding interactions with other complement components such as factor H helps to elucidate its role in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments : Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

See full target information CFB

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 11:154 PubMed32117292

2020

A Systematic Investigation on Complement Pathway Activation in Diabetic Retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Shahna Shahulhameed,Sushma Vishwakarma,Jay Chhablani,Mudit Tyagi,Rajeev R Pappuru,Saumya Jakati,Subhabrata Chakrabarti,Inderjeet Kaur

Nucleic acids research 44:10974-10985 PubMed27536005

2016

A deafness-associated tRNAAsp mutation alters the m1G37 modification, aminoacylation and stability of tRNAAsp and mitochondrial function.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wang,Yanyan Peng,Jing Zheng,Binjiao Zheng,Xiaofen Jin,Hao Liu,Yong Wang,Xiaowen Tang,Taosheng Huang,Pingping Jiang,Min-Xin Guan

Transplantation 96:791-9 PubMed23958924

2013

Activation of the lectin pathway of complement in pig-to-human xenotransplantation models.

Applications

Unspecified application

Species

Unspecified reactive species

Anjan K Bongoni,David Kiermeir,Hansjörg Jenni,Annegret Wünsch,Andrea Bähr,David Ayares,Jörg D Seebach,Eckhard Wolf,Nikolai Klymiuk,Mihai A Constantinescu,Esther Vögelin,Robert Rieben

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Factor B antibody

Western blot - Anti-Factor B antibody (AB72658)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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